The Oil Field Microbiome – How Can M etagenomics H elp the Oil I ndustry?

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John Crane Proprietary The Oil Field Microbiome – How Can Metagenomics Help the Oil Industry? Isaac Newton Institute Open for Business event - 14 th April 2014 Richard Johnson – Senior Technologist - SMITHS CONFIDENTIAL -

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The Oil Field Microbiome – How Can M etagenomics H elp the Oil I ndustry?. Isaac Newton Institute Open for Business event - 14 th April 2014. Richard Johnson – Senior Technologist. What are microbes doing in oil fields? - Souring. H 2 S. - PowerPoint PPT Presentation

Transcript of The Oil Field Microbiome – How Can M etagenomics H elp the Oil I ndustry?

Page 1: The Oil Field  Microbiome  – How Can  M etagenomics  H elp the Oil  I ndustry?

John Crane Proprietary

- SMITHS CONFIDENTIAL -

The Oil Field Microbiome – How Can Metagenomics Help the Oil Industry?Isaac Newton Institute Open for Business event - 14th April 2014

Richard Johnson – Senior Technologist

Page 2: The Oil Field  Microbiome  – How Can  M etagenomics  H elp the Oil  I ndustry?

John Crane Proprietary - SMITHS CONFIDENTIAL -

What are microbes doing in oil fields? - Souring

H2S

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John Crane Proprietary - SMITHS CONFIDENTIAL -

What are microbes doing in oil fields? - Souring

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John Crane Proprietary - SMITHS CONFIDENTIAL -

What are microbes doing in oil fields? - Corrosion

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John Crane Proprietary - SMITHS CONFIDENTIAL -

What are microbes doing in oil fields? - Corrosion

From: Larsen, J., Rasmussen, K., Pedersen, H., Sørensen, K., Lundgaard, T. and Skovhus, T.L. 2010. “Consortia of MIC Bacteria and Archaea Causing Pitting Corrosion in Top Side Oil Production Facilities”, NACE Corrosion, 10252.

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John Crane Proprietary - SMITHS CONFIDENTIAL -

What are microbes doing in oil fields? - Other

Filter plugging Reservoir plugging Reducing separation efficiency Reducing product quality

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John Crane Proprietary - SMITHS CONFIDENTIAL -

An oil field cycle

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John Crane Proprietary - SMITHS CONFIDENTIAL -

Monitoring and mitigation

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John Crane Proprietary - SMITHS CONFIDENTIAL -

Analysis – A paradigm shift – Bug Bottles

SRB Test

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GAB/GAnB Test

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John Crane Proprietary - SMITHS CONFIDENTIAL -

Analysis – A paradigm shift - qPCR

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John Crane Proprietary - SMITHS CONFIDENTIAL -

Analysis – A paradigm shift - NGS

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John Crane Proprietary - SMITHS CONFIDENTIAL -

How does this help?

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John Crane Proprietary - SMITHS CONFIDENTIAL -

How can metagenomics help the oil industry?

Answer the questions...

What microorganisms are in there?How many are there (how big is the problem)?

What are they doing?How can we stop them?

Are there any trends/patterns?How interrelated are oil reservoirs/parts of a system?

Page 14: The Oil Field  Microbiome  – How Can  M etagenomics  H elp the Oil  I ndustry?

John Crane Proprietary - SMITHS CONFIDENTIAL -

Thank you