Post on 16-Dec-2015
Need
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Need
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Alzheimer’s Disease
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http://www.alzheimer.ca/english/disease/whatisit-intro.htm
Age-Dependent Neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila
Greeve, and Kretzschmar,
y-Secretase/presenilin inhibitors for Alzheimer’s disease phenocopy Notch mutations in Drosophila1By: MICCHELLI, and ESLER
Dissecting the pathological effects of human A40 and A42 in Drosophila: A potential model for Alzheimer’s disease
By: Iijima and Liu
Neuroserpin Binds A and Is a Neuroprotective Component of Amyloid Plaques in Alzheimer Disease
By: Kinghorn and Crowther
Purpose
Preservatives will cause an increase in the development of Alzheimer's
symptoms
Hypothesis
The purpose of this experiment was to find if preservatives have any effect on
the development of Alzheimer's disease.
Methodology
By increasing the levels of preservatives (Calcium propionate) given to drosophila models and also exposing those to certain levels of stress will begin to worsen the symptoms of Alzheimer’s and/or begin to develop the symptoms of Alzheimer’s
Increased levels of Calcium propionate and also environmental stress will lead to the development of the plates in drosophila and eventually lead to death due to
Alzheimer’s development.
Normal Drosophila fed normal food
Normal Drosophila fed food with Calcium propionate added
Data will be collected everyday over a 3 month period, a total of 5 trials will be run
Drosophila fed normal food exposed to stress
Drosophila exposed to stress fed food with Calcium propionate added
The means will be calculated and run through statistical analysis (one way ANOVA followed by Scheffe Posthoc test)
BudgetItem Vender Item
NumberQuantity
Description
Price Total Price
Drosophila VialsCarolina Biology Catalog 173120 4 12 vials per order $7.50 $30.00
Drosophila FoodCarolina Biology Catalog 173200 1
Formula 4-24® Instant Medium, Plain, 1 L $6.95 $6.95
APP Drosophila
PBSFisher Sciences Education BP2438-4 5
A standard phosphate buffer $95.56 $477.80
acetoneCarolina Biology Catalog 841481 5
Clear, colorless, flammable liquid $6.50 $32.50
methanolCarolina Biology Catalog 874950 5 flammable $5.75 $28.75
formalin Carolina Biology Catalog 863463 5 flammable $21.95 $109.75
xyleneCarolina Biology Catalog 898741 5 flammable $10.60 $53.00
ethanolCarolina Biology Catalog 861261 5
denatured with isopropanol and methanol $7.95 $39.75
distilled waterCarolina Biology Catalog 858621 5 Distilled Water $7.25 $36.25
Serum Antibody SetCarolina Biology Catalog 202102 5
Goat antisera containing immunoglobulin $99.00 $495.00
Disposable Lab Pack: Carolina Biology Catalog 632920 1
50 Slides,50 Plastic Coverslips,Lens Paper Booklet $11.75 $11.75
i-Brite Plus Neuromics SF40000 5anti-fade mounting media 10 ml Price not yet known Price not yet known
Calcium propionate honeyvill grains 27-816 5
discount 4 to 9: $89.29, 10 to 50: $84.59 $89.99 $89.29
Phosphate Buffer Solution
Carolina Biology Catalog 746864 5
0.1 M Phosphate Buffer Solution $8.50 $40
Do Ability
•All materials are orderable from school approved companies
•Drosophila can be kept alive with relative ease in school lab
Bibliography• Crag A. Michelli, William P Esler and W. Taylor Kimberley, Y-Secretase/presenilin
inhibitors for Alzheimer’s disease phenocopy Notch mutations in Drosophila, FASEB, 3/09/2003, volume 17, pgs 79-81
• Crowther DC, Kinghorn KJ, Miranda E, Page R, Curry JA, et al. (2005) Intraneuronal Ab, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer’s disease. Neuroscience 132: 123–135.
• Harry EJ, Pogliano K, Losick R (1995) Use of immunofluorescence to visualize cellspecific gene expression during sporulation in Bacillus subtilis. J Bacteriol
• 177, volume 12, pgs 3386-93. • Gritta Tettweiler, Mathieu Miron, Mark Jenkins, Nahum Sonenberg, and Paul F. Lasko,
Starvation and oxidative stress resistance in Drosophila are mediated through the eIF4E-binding protein, d4E-BP, Genes Dev, August 15 2005, volume 19, pgs 1840–1843.
• Isabell Greeve, Doris Kretzschmar, Jakob-Andreas Tscha¨pe, Anika Beyn, Claire Brellinger, Michaela Schweizer, Roger M. Nitsch, and Rita Reifegerste1, Age-Dependent neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila, The Journal of Neuroscience, April 21, 2004, volume 16, pgs 3899 –3906
• Koichi Iijima, Hsin-Ping Liu, Ann-Shyn Chiang, Stephen A. Hearn, Mary Konsolaki, Yi ZhongDissecting the pathological effects of human Aβ40 and Aβ42 in Drosophila: A potential model for Alzheimer's disease, PNAS, October 31, 2003, volume 101, pgs 6623–6628
• MUHAMMAD ARSHAD, MARIE PAULSSON, and PETR DEJMEK, Rheology of Buildup, of Acid Casein Gels Breakdown, and Rebodying, March 3 1993, J Dairy Sci volume 76, pgs 331-3316
• P. Mpountoukas, A. Vantarakis, E. Sivridis and T. Lialiaria, Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives, January 30 2008, Food and Chemical Toxicology, volume 46, pgs 2390-2393
• Pogliano K, Harry E, Losick R (1995) Visualization of the subcellular location of• sporulation proteins in Bacillus subtilis using immunofluorescence microscopy. Mol• Microbiol, volume 18, pgs 459-70• Yihong Ye and Mark E. Fortini Apoptotic Activities of Wild-type and Alzheimer’s Disease-
related Mutant Presenilins in Drosophila melanogaster, The Journal of Cell Biology, September 20 1999, Volume 146, pgs 1351–1364