The Effect of Allium Sativum, Camellia

Sinensis and Penicillin on the

Growth of Escherichia Coli Bacteria

Stephanie Close – Sterling Heights High School

Claire St. Louis – Warren Mott High School

Why

?• Antibiotic resistance• Household remedies

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Bac

kgro

und

• Escherichia Coli– What is E.Coli bacteria?– Reproduction process

http://fineartamerica.com/art/photographs/petri+dish+culture+e.coli/all

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und

• Penicillin– What is penicillin?– How it kills bacteria

http://en.wikipedia.org/wiki/Penicillin

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und

• Allium Sativum– What is Allium Sativum?– Antibacterial Agents

http://carolcassara.com/2013/11/how-to-roast-garlic.html

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Bac

kgro

und

• Camellia Sinensis– What is Camellia Sinensis?– Antibacterial Agents

http://www.wisegeek.com/what-is-camellia.htm#

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und

• Zone of Inhibition

http://www.antimicrobialtestlaboratories.com/Zone_of_Inhibition_Test_for_Antimicrobial_Activity.htm

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Prob

lem

• To determine the effect of garlic powder, green tea, and penicillin on the growth of E.coli bacteria

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Hyp

othe

sis

• Penicillin (g) will create the largest zone of inhibition (mm) while green tea (g) will create the smallest zone of inhibition (mm)

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Mat

eria

ls E.Coli

Garlic PowderGreen Tea

Petri Dishes Coffee FiltersPenicillin

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Proc

edur

es

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Dat

a

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Treatment Overall Average Standard Deviation Penicillin 1.1 0.3

Garlic Powder 1.1 0.2Green Tea 1.6 0.2

Obs

erva

tions

• Large bacteria colonies• Green tea stain• Undissolved garlic powder

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Anal

ysis

Zone of Inhibition (mm)

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2 S

ampl

e t-T

est

• Assumptions- 2 SRS from two distinct populations- Populations are independent - Normal Distribution- Population means and population

standard deviations are not known

2

22

1

21

21

ns

ns

xx

t =

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2

22

1

21

21

ns

ns

xx

t =

Con

clus

ion

• Ho: μ1 = μ2

• Ha: μ1 ≠ μ2

• Garlic Powder vs. Penicillin- Failed to reject Ho

• Green Tea vs. Penicillin- Reject Ho

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Err

ors

• Treatment Solutions• Placement of treatment

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Con

clus

ion

• Hypothesis: Rejected• Green tea created the largest zone of

inhibition

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Furt

her

Res

earc

h• Various factors• DOE test

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Rec

ap• Problem• Hypothesis• Conclusion

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Res

ourc

es• http://www.elmhurst.edu/~chm/vchembook/652penicillin.html• http://allicincenter.com/pdf/Allicin.pdf• http://www.webmd.com/vitamins-and-supplements/black-tea-uses-

and-risks.com• http://www.westminster.edu/acad/sim/pdf/

santibioticinhibitionofbacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com• http://www.nlm.nih.gov/medlineplus/druginfo/meds/a682098.html• http://www.microbelibrary.org/component/resource/laboratory-test/

3085-preparing-spread-plates-protocols.com• http://www.studymode.com/essays/The-Effects-Of-Black-Tea-On-

1164976.html• http://www.all-sciencefairprojects.com/science_fair_pr ojects.html• http://www.ncbi.nlm.nih.gov/pubmed/23847105.com• http://www.ncbi.nlm.nih.gov/pubmed/15260343.com• http://www.drlwilson.com/articles/antibiotics.htm• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://community.middlebury.edu/~sontum/chemistry/students/

atteridge/penicillin/penicillin.html• http://www.nutrimart.com/bulk/description/greentea.htm• http://examine.com/supplements/Green+Tea+Catechins/• http://www.ncbi.nlm.nih.gov/pubmed/21827739• http://healthyeating.sfgate.com/egcg-green-tea-8453.html• http://www.drhoffman.com/page.cfm/118• http://www.amazon.com/Foods-Green-Extract-400mg-Vcaps/dp/

B001DNV5CA• http://royalmajestea.com/basics.htm• http://community.middlebury.edu/~sontum/chemistry/students/

atteridge/penicillin/penicillin.html• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://www.teavivre.com/info/green-tea-history/• http://www.westminster.edu/acad/sim/pdf/santibioticinhibition/

bacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com

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Ackn

owle

dgm

ents

• Mr. Estapa • Mrs. Gravel• Bradley Rabquer

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