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Protein structure predictionProtein structure prediction((HBsAgHBsAg)) 25

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((HBsAgHBsAg)) 2

ByMansour PoorebrahimMansour Poorebrahim

PhD, Medical Biotechnology Tehran University of Medical Sciences 2Tehran University of Medical Sciences 2

Hierarchicalnatureofproteinstructure

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33

HBsAg

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44

Methodsforproteinstructureprediction

Comparative modeling

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Comparative modeling

Threading

2

Ab initio prediction

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HHpred serverHHpred server

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66

Threading(structuralfoldrecognition)g g

Protein threading predicts the 3D structure for a new

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protein by aligning its primary sequence to proteins in thePDB to see if a similar structure can be found

2

A method of protein modeling to model proteins which donot have homologous proteins and have the same fold asproteins of known structuresproteins of known structures.

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Threading(structuralfoldrecognition)g g

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PHYRE2serverhttp://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index

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99

Modelvisualization

UCSF chimera

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1010

ProteinvalidationServer

RAMPAGE

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1111

PredictedmodelsofHBsAgbyPHYRE2g y

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1212

HBsAg

T123I (9/31)

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( / )

2

1313

PredictedmodelsofHBsAgbyPHYRE2g y

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1414

3/05

/201

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