PCR

PCRPCR & ITS & ITS APPLICATIONSAPPLICATIONS

3Polymerase Chain Reaction

PCR means Polymerase Chain Reaction

It is a technique used to amplify the DNA

It was developed in 1984 by Kary Mullis

In 1993 Mullis won the Nobel Prize

Thermal cycler is an apparatus used to carryout a PCR reaction

Minute amount of starting material

Quick and simple

Extremely sensitive

Components required for a Components required for a PCR reactionPCR reaction

Taq DNA polymerases

Target sequences Deoxyribonucleoside

triphosphates

Oligomer primers

Amplification buffer5Polymerase Chain Reaction

Procedure - PCR cycleProcedure - PCR cycle

Procedure - Polymerase Chain reaction

DenaturationAnnealingDna synthesis

DNA cloning DNA cloning for sequencingfor sequencing DNA-based DNA-based Phylogeny Phylogeny Functional analysis of Functional analysis of GenesGenesThe diagnosis of The diagnosis of Hereditary Hereditary diseasesdiseasesThe identification of The identification of Genetic Genetic Fingerprints Fingerprints ( (used in forensic sciences and used in forensic sciences and paternity testingpaternity testing))The detection and diagnosis of The detection and diagnosis of Infectious diseasesInfectious diseases

Polymerase Chain Reaction 11

What is PCR?What is PCR?

What is the use of PCR?What is the use of PCR? Protocol Protocol

Applications Applications

Polymerase chain reactionPolymerase chain reaction

PCR

Education

Transcript of PCR

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PCR, RT-PCR, FISH

The polymerase chain reaction (PCR). Experiment Goals Understand how PCR technique works. Perform PCR experiment. Analyze PCR products.

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RealTime PCR VS Traditional PCR

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What is PCR? PCR