PCR

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1 PCR PCR & ITS & ITS APPLICATIONS APPLICATIONS

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REGARDING PCR

Transcript of PCR

Page 1: PCR

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PCRPCR   & ITS & ITS APPLICATIONSAPPLICATIONS

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3Polymerase Chain Reaction

PCR means Polymerase Chain Reaction

It is a technique used to amplify the DNA

It was developed in 1984 by Kary Mullis

In 1993 Mullis won the Nobel Prize

Thermal cycler is an apparatus used to carryout a PCR reaction

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4Polymerase Chain Reaction

Minute amount of starting material

Quick and simple

Extremely sensitive

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Components required for a Components required for a PCR reactionPCR reaction

Taq DNA polymerases

Target sequences Deoxyribonucleoside

triphosphates

Oligomer primers

Amplification buffer5Polymerase Chain Reaction

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Procedure - PCR cycleProcedure - PCR cycle

6Polymerase Chain Reaction

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Procedure - Polymerase Chain reaction

7Polymerase Chain Reaction

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Procedure - Polymerase Chain reaction

8Polymerase Chain Reaction

DenaturationAnnealingDna synthesis

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Procedure - Polymerase Chain reaction

9Polymerase Chain Reaction

DenaturationAnnealingDna synthesis

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DNA cloning DNA cloning for sequencingfor sequencing DNA-based DNA-based Phylogeny Phylogeny Functional analysis of Functional analysis of GenesGenesThe diagnosis of The diagnosis of Hereditary Hereditary diseasesdiseasesThe identification of The identification of Genetic Genetic Fingerprints Fingerprints ( (used in forensic sciences and used in forensic sciences and paternity testingpaternity testing))The detection and diagnosis of The detection and diagnosis of Infectious diseasesInfectious diseases

10Polymerase Chain Reaction

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Polymerase Chain Reaction 11

What is PCR?What is PCR?

What is the use of PCR?What is the use of PCR? Protocol Protocol

Applications Applications

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Polymerase chain reactionPolymerase chain reaction

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Polymerase Chain Reaction 13

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Polymerase Chain Reaction 15


Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR

Advanced PCR Dave Palmer, Byotix, Inc.. Advanced PCR PCR of Plant Material Multiplex PCR Modifications to Standard PCR PCR Troubleshooting.

Advanced PCR Dave Palmer, Byotix, Inc.. Advanced PCR PCR of Plant Material Multiplex PCR Modifications to Standard PCR PCR Troubleshooting.

Nested PCR,Fusion primers, Integrated PCR

Nested PCR,Fusion primers, Integrated PCR

PCR and quantitative-PCR protocol Description ... · PCR and quantitative-PCR protocol Description: qPCR is a method of quantifying DNA based on PCR (polymerase chain reaction). qPCR

PCR and quantitative-PCR protocol Description ... · PCR and quantitative-PCR protocol Description: qPCR is a method of quantifying DNA based on PCR (polymerase chain reaction). qPCR

PCR, RT-PCR and qPCR

PCR, RT-PCR and qPCR

PCR - A New Flow Metric · PCR and Observation Domains WorkGroup Based PCR Comprehensive Layer 3 - 7 Flow PCR Aggregated Layer 3 PCR System Based PCR Measurement Highly Granular Comprehensive

PCR - A New Flow Metric · PCR and Observation Domains WorkGroup Based PCR Comprehensive Layer 3 - 7 Flow PCR Aggregated Layer 3 PCR System Based PCR Measurement Highly Granular Comprehensive

PCR quantitative en temps réel Lydie Pradel. PCR.

PCR quantitative en temps réel Lydie Pradel. PCR.

INTRODUCTION TO REVERSE TRANSCRIPTION PCR (RT-PCR)hpc.ilri.cgiar.org/beca/bioinfo/docs/RT-PCR Principle-ABCF 2016... · RT-PCR refers to PCR that uses product of an Reverse Transcription

INTRODUCTION TO REVERSE TRANSCRIPTION PCR (RT-PCR)hpc.ilri.cgiar.org/beca/bioinfo/docs/RT-PCR Principle-ABCF 2016... · RT-PCR refers to PCR that uses product of an Reverse Transcription

PCR, real-time PCR, reverse transcription, and cloning

PCR, real-time PCR, reverse transcription, and cloning

Diagnostic strategy in genetically determined diseases ... Karyotype analysis FISH CGH MLPA QF-PCR Methods: PCR MLPA Sequencing PCR-RLFP PCR-Multiplex PCR-ASO Methods: ... The user

Diagnostic strategy in genetically determined diseases ... Karyotype analysis FISH CGH MLPA QF-PCR Methods: PCR MLPA Sequencing PCR-RLFP PCR-Multiplex PCR-ASO Methods: ... The user

Variants of PCR Lecture 4. Colony PCR Multiplex PCR Nested PCR Reverse transcriptase PCR Quantative PCR Hot Start PCR Touch down PCR.

Variants of PCR Lecture 4. Colony PCR Multiplex PCR Nested PCR Reverse transcriptase PCR Quantative PCR Hot Start PCR Touch down PCR.

SEQUENCIAMENTO DO DNA. SEQUENCIAMENTO PCR PCR PCR.

SEQUENCIAMENTO DO DNA. SEQUENCIAMENTO PCR PCR PCR.

Progene PCR QUOTE: Q4-2019 PCR Products

Progene PCR QUOTE: Q4-2019 PCR Products

PCR - Bibliotheca Alexandrina · PCR The different steps of PCR PCR DNA sequencing Microarrays Mass-spec PCR Exponential increase of the number of copies during PCR PCR DNA sequencing

PCR - Bibliotheca Alexandrina · PCR The different steps of PCR PCR DNA sequencing Microarrays Mass-spec PCR Exponential increase of the number of copies during PCR PCR DNA sequencing

Maximizing PCR and RT-PCR successwiki.biol.uw.edu.pl › t › img_auth.php › 7 › 7d › PCR_qiagen.pdf · Maximizing PCR and RT-PCR success Addressing critical factors and new

Maximizing PCR and RT-PCR successwiki.biol.uw.edu.pl › t › img_auth.php › 7 › 7d › PCR_qiagen.pdf · Maximizing PCR and RT-PCR success Addressing critical factors and new

Pcr & Real time pcr

Pcr & Real time pcr

Παρουσίαση του PowerPoint€¦ · Successful PCR: everything is optimized •Template DNA: ... RT-PCR microfluidic PCR microfluidics drawbacks: PCR inhibition and carryover

Παρουσίαση του PowerPoint€¦ · Successful PCR: everything is optimized •Template DNA: ... RT-PCR microfluidic PCR microfluidics drawbacks: PCR inhibition and carryover

CALENDRIER DES FORMATIONS PCR 2018 - C2i industrie · jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs

CALENDRIER DES FORMATIONS PCR 2018 - C2i industrie · jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr r jrs pcr i pcr r jrs pcr i pcr r jrs pcr i pcr r jrs

AMPLIFICATION PCR and RT-PCR Reagents - HPSThpst.cz/sites/default/files/attachments/pcr-and-rt-pcr-reagents.pdf · 0>0 PCR AND RT-PCR REAGENTS We have the best PCR and RT-PCR reagents

AMPLIFICATION PCR and RT-PCR Reagents - HPSThpst.cz/sites/default/files/attachments/pcr-and-rt-pcr-reagents.pdf · 0>0 PCR AND RT-PCR REAGENTS We have the best PCR and RT-PCR reagents

(Provisional Translation) Product Category Rules (PCR ...pcr-library.edf.org.tw/data/japan/PCR PA-AG-01.pdf · (Provisional Translation) Product Category Rules (PCR) Approved PCR

(Provisional Translation) Product Category Rules (PCR ...pcr-library.edf.org.tw/data/japan/PCR PA-AG-01.pdf · (Provisional Translation) Product Category Rules (PCR) Approved PCR