LLLLABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIMENTAL BOTANYMENTAL BOTANYMENTAL BOTANYMENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA DEPARTMENT OF BIOLOGY http://botanika.biologija.org/exp/ contact email: ales.kladnik@bf.uni ales.kladnik@bf.uni ales.kladnik@bf.uni ales.kladnik@bf.uni----lj.silj.silj.silj.si

Last change: 2007-02-01 1/1

PHOSPHATE BUFFERED SALINE (PBS)

10x PBS (sodium-potassium combo), 1 L:

• 2 g KCl

• 2.4 g KH2PO4

• 80 g NaCl

• 11.45 g Na2HPO4 note: if Na2HPO4 (7H2O), use 21.6g, if Na2HPO4 (12H2O), use 28.8g.

Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml.

Autoclave and store at room temperature.

10x PBS, pH 7˙4 (sodium), 100 mL:

• 7,6 g NaCl (Mw 58.4425 g/mol, M = 1,3 mol/L)

• 0,99 g Na2HPO4 (Mw 141.959 g/mol, M = 0,07 mol/L)

• 0,41 g NaH2PO4 x H2O (Mw 137.992 g/mol, M = 0,03 mol/L)

Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room

temperature.

Calculation of Mw: CHEMIX (http://home.c2i.net/astandne/help_htm/chemix.htm)