PBS Buffer

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LABORATORY FOR EXPERI ABORATORY FOR EXPERI ABORATORY FOR EXPERI ABORATORY FOR EXPERIMENTAL BOTANY MENTAL BOTANY MENTAL BOTANY MENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA DEPARTMENT OF BIOLOGY http://botanika.biologija.org/exp/ contact email: [email protected] [email protected] [email protected] [email protected] lj.si lj.si lj.si Last change: 2007-02-01 1/1 PHOSPHATE BUFFERED SALINE (PBS) 10x PBS (sodium-potassium combo), 1 L: • 2 g KCl • 2.4 g KH 2 PO 4 • 80 g NaCl • 11.45 g Na 2 HPO 4 note: if Na 2 HPO 4 (7H 2 O), use 21.6g, if Na 2 HPO 4 (12H 2 O), use 28.8g. Dissolve everything in approximately 800 ml of dH 2 O, then fill up to 1000 ml. Autoclave and store at room temperature. 10x PBS, pH 7˙4 (sodium), 100 mL: • 7,6 g NaCl (Mw 58.4425 g/mol, M = 1,3 mol/L) • 0,99 g Na 2 HPO 4 (Mw 141.959 g/mol, M = 0,07 mol/L) • 0,41 g NaH 2 PO 4 x H 2 O (Mw 137.992 g/mol, M = 0,03 mol/L) Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room temperature. Calculation of Mw: CHEMIX (http://home.c2i.net/astandne/help_htm/chemix.htm )

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Transcript of PBS Buffer

Page 1: PBS Buffer

LLLLABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIMENTAL BOTANYMENTAL BOTANYMENTAL BOTANYMENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA DEPARTMENT OF BIOLOGY http://botanika.biologija.org/exp/ contact email: [email protected] [email protected] [email protected] [email protected]

Last change: 2007-02-01 1/1

PHOSPHATE BUFFERED SALINE (PBS)

10x PBS (sodium-potassium combo), 1 L:

• 2 g KCl

• 2.4 g KH2PO4

• 80 g NaCl

• 11.45 g Na2HPO4 note: if Na2HPO4 (7H2O), use 21.6g, if Na2HPO4 (12H2O), use 28.8g.

Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml.

Autoclave and store at room temperature.

10x PBS, pH 7˙4 (sodium), 100 mL:

• 7,6 g NaCl (Mw 58.4425 g/mol, M = 1,3 mol/L)

• 0,99 g Na2HPO4 (Mw 141.959 g/mol, M = 0,07 mol/L)

• 0,41 g NaH2PO4 x H2O (Mw 137.992 g/mol, M = 0,03 mol/L)

Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room

temperature.

Calculation of Mw: CHEMIX (http://home.c2i.net/astandne/help_htm/chemix.htm)

Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for ...ibj.pasteur.ac.ir/article-1-2495-en.pdf · buffer solutions (PBS, pH 7.4 and pH 5.5) at 37 °C. Briefly, the NPs were

Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for ...ibj.pasteur.ac.ir/article-1-2495-en.pdf · buffer solutions (PBS, pH 7.4 and pH 5.5) at 37 °C. Briefly, the NPs were

GeneMedi’sProductsfor VaccinesandDrugDiscoveryagainstCOVID …s products... · PBS liquid in PBS buffer lyophilized lyophilized and stock at room temperature for 7 days before reconstitution

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Supporting Information - PNAS...2011/05/16  · 1× PBS, 10 mM Imidazol and two times for 15 min in the same buffer. The protein was eluted with 1× PBS, 150 mM Imidazole, dialysed

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Intro Media Institutions PBS or Murdoch?PBS or Murdoch? PBS – BBC, PBS America etcPBS – BBC, PBS America etc.

Intro Media Institutions PBS or Murdoch?PBS or Murdoch? PBS – BBC, PBS America etcPBS – BBC, PBS America etc.

Micro PBS PBS for Small Group Instruction

Micro PBS PBS for Small Group Instruction

PBS Portable Batch System - qcd.phys.cmu.eduqcd.phys.cmu.edu › QCDcluster › pbs › pbs_ids.pdf · PBS IDS PBS Revision History Revision 1.0 June, 1994 — Alpha Test Release

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PBS Professional 13.1 User’s Guide - pbsworks.co.kr · About PBS Documentation UG-viii PBS Professional 13.1 User’s Guide Document Conventions PBS documentation uses the following

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Cellular/Molecular CNG-Modulin:ANovelCa … · 2012. 2. 25. · anti-CNG-modulin antibody in PBS buffer containing 0.1% Triton X-100. Slides were then washed three times with PBS

Cellular/Molecular CNG-Modulin:ANovelCa … · 2012. 2. 25. · anti-CNG-modulin antibody in PBS buffer containing 0.1% Triton X-100. Slides were then washed three times with PBS

PBSK SumLearn ActivitySheets › sites › › files › education › ...The PBS KIDS logo & PBS KIDS ® PBS. Used with permission.© LATW Productions Inc. The PBS KIDS logo & PBS

PBSK SumLearn ActivitySheets › sites › › files › education › ...The PBS KIDS logo & PBS KIDS ® PBS. Used with permission.© LATW Productions Inc. The PBS KIDS logo & PBS

DNA Techniques in Wildlife Forensics (Animals) · arising out of the ... deoxy Cytosine triphosphate PBS – Phosphate Buffer ... determine the geographical origin of animal products

DNA Techniques in Wildlife Forensics (Animals) · arising out of the ... deoxy Cytosine triphosphate PBS – Phosphate Buffer ... determine the geographical origin of animal products

Abnormalities in Mitochondrial Structure in Cells from ... · PBS, washed in dilution buffer (PBS 2% BSA 1% normal serum 0.1% Triton-X100), and incubated with 0.5% SDS in PBS to facilitate

Abnormalities in Mitochondrial Structure in Cells from ... · PBS, washed in dilution buffer (PBS 2% BSA 1% normal serum 0.1% Triton-X100), and incubated with 0.5% SDS in PBS to facilitate

BUFFERS AND SOLUTIONS - blossombio.comblossombio.com/pdf/products/CAT_CANDOR.pdf · Washing Buffer “PBS” 10 ... questions. If this is the case for you, ... described in the chapter

BUFFERS AND SOLUTIONS - blossombio.comblossombio.com/pdf/products/CAT_CANDOR.pdf · Washing Buffer “PBS” 10 ... questions. If this is the case for you, ... described in the chapter

PBS Kids PBS Kids by Amanda Sullivan .

PBS Kids PBS Kids by Amanda Sullivan .

PBS Professional 13.1 Reference Guide - pbsworks.co.kr · PBS Professional 13.1 Reference Guide RG-vii About PBS Documentation The PBS Professional Documentation The documentation

PBS Professional 13.1 Reference Guide - pbsworks.co.kr · PBS Professional 13.1 Reference Guide RG-vii About PBS Documentation The PBS Professional Documentation The documentation

Biochemical and Biophysical Research Communications · E-mail addresses: yukinari-k@bea.hi-ho.ne.jp (Y. Kato), takagi@protein.osaka-u. ... perBlock T20 (PBS) Blocking Buffer in PBS

Biochemical and Biophysical Research Communications · E-mail addresses: [email protected] (Y. Kato), [email protected]. ... perBlock T20 (PBS) Blocking Buffer in PBS

Helical apolipoproteins of high-density lipoprotein ... · PDF fileman plasma HDL ( 19, 20 ). ... Cells were washed with ice-cold PBS-CM, lysed with RIPA buffer, ... SiRNAs (Stealth

Helical apolipoproteins of high-density lipoprotein ... · PDF fileman plasma HDL ( 19, 20 ). ... Cells were washed with ice-cold PBS-CM, lysed with RIPA buffer, ... SiRNAs (Stealth

PBS Pro Administrator Guide - CMU QCD Clusterqcd.phys.cmu.edu › QCDcluster › pbs › pbs_pro_admin.pdfconfigure, and manage the Portable Batch System (PBS). PBS is a workload management

PBS Pro Administrator Guide - CMU QCD Clusterqcd.phys.cmu.edu › QCDcluster › pbs › pbs_pro_admin.pdfconfigure, and manage the Portable Batch System (PBS). PBS is a workload management

BBVA COLOMBIA · TAM +6 pbs TAM -16 pbs TAM -53 pbs $44 Billones 11.8% Recursos de Clientes TAM +28 pbs Vista Cuota 12.10% TAM +124 pbs CDT’s Cuota 13.92% TAM +64 pbs Ahorro Cuota

BBVA COLOMBIA · TAM +6 pbs TAM -16 pbs TAM -53 pbs $44 Billones 11.8% Recursos de Clientes TAM +28 pbs Vista Cuota 12.10% TAM +124 pbs CDT’s Cuota 13.92% TAM +64 pbs Ahorro Cuota

Preliminary study of binary protein adsorption … › pdf › ABB_2013062415303253.pdf= 3.7 mol·L −1) in the PBS buffer (pH 7). It was as- sumed that protein-protein interactions

Preliminary study of binary protein adsorption … › pdf › ABB_2013062415303253.pdf= 3.7 mol·L −1) in the PBS buffer (pH 7). It was as- sumed that protein-protein interactions

50' UNDISTURBEDVEGETATIVE BUFFER · 15 6 17 3 25' state buffer 50' undisturbedvegetative buffer 50' undisturbed vegetative buffer 50' undisturbedvegetative buffer asphalt asphalt

50' UNDISTURBEDVEGETATIVE BUFFER · 15 6 17 3 25' state buffer 50' undisturbedvegetative buffer 50' undisturbed vegetative buffer 50' undisturbedvegetative buffer asphalt asphalt