Post on 27-May-2018
New technology
vaccines compatibility
Yannick Gardin,Innovation & Strategy Biology
Director, Ceva Group, France
Introduction
EFFICACY
SAFETY
COMPATIBILITY
Introduction
EFFICACY
SAFETY
COMPATIBILITY
What has changed ?
Huge opportunities from Biotechnology
Vaccination is routine tool of Production
Production & Process > Science
What has changed ?
Huge opportunities from Biotechnology
Vaccination is routine tool of Production
Production & Process > Science
Biotechnology ?
Biotechnology ?
LIVE
ATTENUATED
Biotechnology ?
INACTIVATED
ADJUVANTED
LIVE
ATTENUATED
Biotechnology ?
Biotechnology ?
Epitope
(virulence / protection)
Biotechnology ?
Epitope
(virulence / protection)
Genome
(gene / deletion / insertion)
Biotechnology ?
Carrier
(expression / virulence)
Epitope
(virulence / protection)
Genome
(gene / deletion / insertion)
Biotechnology ?
Antibody
(immune-complex)
Epitope
(virulence / protection)
Genome
(gene / deletion / insertion)
Carrier
(expression / virulence)
Biotechnology ?
Subunit VectorReverse genetics
Immunecomplex
Etc.
Antibody
(immune-complex)
Epitope
(virulence / protection)
Genome
(gene / deletion / insertion)
Carrier
(expression / virulence)
What has changed ?
Huge opportunities from Biotechnology
Vaccination is routine tool of Production
Production & Process > Science
What has changed ?
Huge opportunities from Biotechnology
Vaccination is routine tool of Production
Production & Process > Science
Production & Process > Science
Production & Process > Science
Production & Process > Science
HATCH
Production & Process > Science
HATCH
Production & Process > Science
HATCH TRANSFER
Production & Process > Science
IBD IB
SHS MDND
COCCI
CAMPY AI(H9)
AI(H5)
ILT
SALMO
IBvar.
etc.
AI(H7)
ALL IN ONE
Production & Process > Science
ALL IN ONE
means
Production & Process > Science
EFFICACY
SAFETY
COMPATIBILITY
ALL IN ONE
means
Production & Process > Science
EFFICACY
SAFETY
COMPATIBILITY
Production & Process > Science
EFFICACY
SAFETY
Production & Process > Science
COMPATIBILITY
COMPATIBILITY
Compatibility ?
IBDND
Compatibility ?
IBDND
WHICH CRITERIA ?
Compatibility ?
IBDND
Compatibility ?
Be specific, not general !
Compatibility ?
Level of Immunity
Onset of Immunity
Duration of Immunity
Be specific, not general !
Compatibility ?
Interference
Addition
Synergism
Level of Immunity
Onset of Immunity
Duration of Immunity
Be specific, not general !
Compatibility ?
Interference
Addition
Synergism
Level of Immunity
Onset of Immunity
Duration of Immunity
Be specific, not general !
Significance / Consequences
10
20
30
40
50
60
70
80
90
100
Protection (%)
00 5 10 15 20 25 30 Age (days)35 40 45 50
Maternally Derived Antibodies
Live Respiratory vaccine
rHVT Vector vaccine
Immune Complex vaccine
Compatibility ?
Dynamics of Vaccine Immunity
Compatibility ?
Investigation of Vaccine Immunity variables
Protection against challenge
- various ages, strains, doses, etc.
- clinical protection (mortality,
morbidity, lesions)
- re-excretion of challenge virus
Humoral immunity / Antibody response :
- general / local / assay
Cell-mediated immunity :
- general / local / assay
INVESTIGATION RESULTS
Compatibility ?
Transmune Immune Complex IBD
Vectormune ND rHVT-ND F gene
Vectormune IBD rHVT-IBD VP2 gene
Vectormune AI rHVT-AI HA(H5) gene
Vectormune LT rHVT-LT gB gene
Compatibility ?
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
Broilers with MDA / NDV & IBDV
Detection of vaccine take :- Immune complex : PCR / bursa samples
- rHVT-ND : PCR / spleen samples
NDV challenge : 105 EID50 – Genotype VII str.
- Clinical protection
- HI antibody response
- shedding 4 & 7 dpc at 3 & 6 weeks
vvMDV challenge : RB1B at 8 days
Compatibility ?
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
Compatibility ?
DETECTION OF VACCINE TAKES
rHVT-ND
SPLEEN
10 DPV
W2512 IBDV
BURSA
28 DPV
Immune complex IBD - 90 %
rHVT-ND 100 % -
Immune Complex
+ rHVT-ND100 % 80 %
BROILERS
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
Positivity
limit
n.a. b b a b b a a a
Compatibility ?
NDV ANTIBODY RESPONSE (HI)
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
Compatibility ?
CLINICAL PROTECTION / NDV CHALLENGE
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
b b a b b a a a a a
b b a b b a a a a a
Compatibility ?
SHEDDING OF ND CHALLENGE VIRUS
Challenge at week 3 Challenge at week 6
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
82%
98%
30%
Compatibility ?
CLINICAL PROTECTION / MDV CHALLENGE
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
82%
98%
30%
Compatibility ?
CLINICAL PROTECTION / MDV CHALLENGE
Immune Complex IBD (Transmune)
& rHVT-ND (Vectormune ND)
Compatibility ?
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
Broilers with MDA against NDV, Classical &
Variant E IBDV
Challenges :
- NDV : 105 EID50 – Chimalhuacan str.
- vvIBDV : 105 EID50 – TR isolate
- Variant IBDV : 105 EID50 – Del. E strain
Protection against IBDV assessed 10 dpc
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND
rHVT-IBD
rHVT-ND +
rHVT-IBD
Controls
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND 0 84 100 100
rHVT-IBD
rHVT-ND +
rHVT-IBD
0 37 70 70
Controls
0 0 0 0
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND 0 84 100 100
rHVT-IBDnd 30 nd 80
rHVT-ND +
rHVT-IBD
0 37 70 70
nd 10 nd 40
Controls
0 0 0 0
nd 10 nd 0
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND 0 84 100 100
rHVT-IBDnd 30 nd 80
20 60 90 80
rHVT-ND +
rHVT-IBD
0 37 70 70
nd 10 nd 40
0 10 11 40
Controls
0 0 0 0
nd 10 nd 0
0 0 0 0
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND 0 84 100 100
rHVT-IBDnd 30 nd 80
20 60 90 80
rHVT-ND +
rHVT-IBD
0 37 70 70
nd 10 nd 40
0 10 11 40
Controls
0 0 0 0
nd 10 nd 0
0 0 0 0
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
14 21 28 36
rHVT-ND 0 84 100 100
rHVT-IBDnd 30 nd 80
20 60 90 80
rHVT-ND +
rHVT-IBD
0 37 70 70
nd 10 nd 40
0 10 11 40
Controls
0 0 0 0
nd 10 nd 0
0 0 0 0
% protection / NDV vvIBDV var.E IBDV challenge
BROILERS
rHVT-ND (Vectormune ND)
& rHVT-IBD (Vectormune IBD)
Compatibility ?
rHVT-ND (Vectormune ND)
& rHVT-LT (Vectormune LT)
Compatibility ?
Broilers
Challenges at 3 weeks PV
NDV : NDV B1 strain – Eye drop
- Re-isolation from trachea 5 dpc
ILTV : 103 EID50 – USDA challenge str.
- Clinical protection over 10 dpc
rHVT-ND (Vectormune ND)
& rHVT-LT (Vectormune LT)
Compatibility ?
ND PROTECTION%
rHVT-ND (Vectormune ND)
& rHVT-LT (Vectormune LT)
Compatibility ?
LT PROTECTION
0102030405060708090
rHVT LT rHVT LT + VTM HVT NDV
rHVT LT + VTM HVT NDV
CHALLENGE CONTROL
%
rHVT-ND (Vectormune ND)
& rHVT-LT (Vectormune LT)
Compatibility ?
LT PROTECTION
0102030405060708090
rHVT LT rHVT LT + VTM HVT NDV
rHVT LT + VTM HVT NDV
CHALLENGE CONTROL
%
rHVT-ND (Vectormune ND)
& rHVT-LT (Vectormune LT)
Compatibility ?
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
SPF chickens
Challenges :
- NDV : 105 EID50 – Genotype VII Malaysian str.
- HPAIV : 106 EID50 – Egypt 2008 H5N1 strain
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
28 56
rHVT-ND
rHVT-AI
rHVT-ND +
rHVT-AI
Controls
% protection / NDV HPAIV challenge
SPF
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
28 56
rHVT-ND 100 100
rHVT-AI
rHVT-ND +
rHVT-AI
100 100
Controls0 0
% protection / NDV HPAIV challenge
SPF
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
28 56
rHVT-ND 100 100
rHVT-AI 100 100
rHVT-ND +
rHVT-AI
100 100
100 90
Controls0 0
0 0
% protection / NDV HPAIV challenge
SPF
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
Layer pullets with MDA / NDV & H5N1 AIV
Challenges :
- NDV : 105 EID50 – Genotype VII Malaysian str.
- HPAIV : 106 EID50 – Egypt 2008 H5N1 strain
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
% protection / NDV HPAIV challenge
21 28 49 70
rHVT-ND
rHVT-AI
rHVT-ND +
rHVT-AI
Controls
LAYERS
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
% protection / NDV HPAIV challenge
21 28 49 70
rHVT-ND 90 100 100 100
rHVT-AI
rHVT-ND +
rHVT-AI
85 85 95 100
Controls15 0 0 0
LAYERS
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
21 28 49 70
rHVT-ND 90 100 100 100
rHVT-AI nd 0 100 80
rHVT-ND +
rHVT-AI
85 85 95 100
nd 20 80 90
Controls15 0 0 0
nd 0 0 0
% protection / NDV HPAIV challenge
LAYERS
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
Compatibility ?
21 28 49 70
rHVT-ND 90 100 100 100
rHVT-AI nd 0 100 80
rHVT-ND +
rHVT-AI
85 85 95 100
nd 20 80 90
Controls15 0 0 0
nd 0 0 0
% protection / NDV HPAIV challenge
LAYERS
rHVT-ND (Vectormune ND)
& rHVT-AI (Vectormune AI)
CONCLUSIONS
Biotechnology has given us the possibility to
have chickens vaccinated against many
diseases in only one (broilers) or two (layers,
breeders) vaccination sessions : at hatch and
at transfer.
This will perfectly answer Production and
Process constraints of the poultry industry.
This described situation is stressing the
importance of Compatibility studies between
specific vaccines. General assumptions are
not advised.
Conclusions (1)
Detailed studies are needed to Investigate
various aspects of protection included in the
Compatibility concept.
Results are seldom black or white :
- they need to be discussed,
- consequences weighted,
- so that reliable, sound and clear
recommendations can be given to users
Final decision is often an acceptable
compromise and need to be adapted to
different field situations.
Conclusions (2)
According to experiments conducted to date,
we concluded to :
Compatibility between :
- Transmune & Vectormune ND
- Transmune & Vectormune FP-LT
- Vectormune ND & Vectormune AI (&
Transmune)
- Vectormune ND & Vectormune FP-LT
No compatibility between :
- Vectormune ND & Vectormune IBD
- Vectormune ND & Vectormune LT
Conclusions (3)
Regarding combination with Live & Inactivated
classical vaccines, we concluded to :
Compatibility between :
- Cevac Vitabron L (live ND & IB - given by
spray & Transmune & Vectormune range
- Cevac MD SB-1 or Cevac MD Rispens
vaccines & Vectormune (HVT) range
- Transmune & Cevac Broiler ND K
(Inactivated ND vaccine)
Conclusions (4)
MANY THANKS TO
CEVA PARTNERS
& CEVA COLLEAGUES
Vectormune ND / Vectormune AI
THANK YOU
FOR YOUR ATTENTION
Vectormune ND / Vectormune AI