Modeling Focused Beam

Propagation in scattering media

Janaka Ranasinghesagara

Teaching Objectives

► The need for computational models of focused beam propagation in

scattering medium

► Introduction to the principles and mathematical models underlying

focus beam propagation.

► Learn how fundamental concepts are applied to develop an efficient

focused beam propagation model.

Focused Beam Propagation in free space

Amp.

Phase

Focused Beam Propagation in free space

Analytical solution Richards and Wolf. Proc. Royal Soc. Lond. A 253(1274) 1959

Amp.

Phase

Focused Beam Propagation in scattering medium

• Scattering distorts the excitation volume

• Scattering is the main limiting factor for penetration depth in

microscopy

Focused Beam Propagation in scattering medium

• Scattering distorts the excitation volume

• Scattering is the main limiting factor for penetration depth in

microscopy

Spatial scales of Imaging and Models

Ntziachristos, Nat. Methods, 7 (2010)

Leigh, Chen and Liu, Biomed Opt. Exp., 5 (6) (2014)

Existing Focused Beam Propagation Models

Finite Difference Time Domain (FDTD)

Solution for Maxwell’s equations

• Solve Maxwell’s equations rigorously in a

voxelized space

• Size of the voxel has to be small

• Need enormous computational resources

(15m×15m ×50m 12GB, 500hours*)

• “Stair case” errors

*Starosta and Dunn, Opt. Express 17(15), (2009)

Capoglu et al. Opt. Express 21(1), (2013)

Elmaklizi et al. JBO 19(7) (2014) http://www.angorafdtd.org/

Existing Focused Beam Propagation Models

Finite Difference Time Domain (FDTD)

Solution for Maxwell’s equations

• Solve Maxwell’s equations rigorously in a

voxelized space

• Size of the voxel has to be small

• Need enormous computational resources

(15m×15m ×50m 12GB, 500hours*)

• “Stair case” errors

*Starosta and Dunn, Opt. Express 17(15), (2009)

Capoglu et al. Opt. Express 21(1), (2013)

Elmaklizi et al. JBO 19(7) (2014) http://www.angorafdtd.org/

Monte Carlo Simulation

• Propagates photons towards the focal

point

• Ignores the wave nature of light

• Considers far-field phase function

• Provides mean behavior

• Requires large number of photons

Song et al, Appl. Opt.. 38(13) (1999)

Blanca et. Al Appl. Opt. 37(34) (1998)

Dunn et al, Appl. Opt.. 39(7) (2000)

Hayakawa et al, Biomed. Opt. Exp. 2(2) (2011)

Cai et al, Prog. in Electromag. Res. 142 (2013)

Cai et al, JBO, 19(1) (2014)

►Electromagnetic wave

►Maxwell’s equations

►Plane wave solution to Maxwell’s

equations

►Properties of plane wave

Key Concepts, Equations and Properties

Light is an Electromagnetic Wave

Maxwell’s Equations

► Provide exact model for EM wave propagation

► Provide theoretical foundation of optics

► Model wave interference, diffraction and polarization

0t

HE

0t

EH J

0

E

0 H

(Faraday’s Law)

(Gauss’ Law)

(Gauss’ Law

for Magnetism)

(Ampere’s Law)

𝐄: Electric field

𝐇: Magnetic field

𝐉: Current density

𝜌: Charge density

𝜇0: Permeability

𝜀0: Permittivity

Origin of Wave Equation from Maxwell’s Equations

► Provide exact model for EM wave propagation

► Provide theoretical foundation of optics

► Model wave interference, diffraction and polarization

0t

HE

0t

EH J

0

E

0 H

(Faraday’s Law)

(Gauss’ Law)

(Gauss’ Law

for Magnetism)

(Ampere’s Law) 0t

EH

0 E

No free

charges

No flow

of current

22

0 0 2t

EE

Wave equation

in free space

22

2 2

1

c t

EE

where

0 0

1c

Plane Wave

22

2 2

1

c t

EE

Plane wave solution

( ) cos E 0z E k z ct

( ) sin E 0z E k z ct

OR

Wave equation

Plane Wave

( ) cos E 0z E kz t

2

0 0

1 1Re

2 2Intensity cE E H

k: wave number = 2/

𝜔: angular frequency

𝜀0: Permittivity

c: Speed of light in vacuum

Amplitude Phase

Amplitude

Phase

Plane wavefront

22

2 2

1

c t

EE

Plane wave solution

( ) cos E 0z E k z ct

( ) sin E 0z E k z ct

OR

Wave equation

Complex Representation of Waves

( ) cos sin E 0 0z E kz t iE kz t

Amplitude =

Im ( )

Re ( )

zArcTan kz t

z

E

EPhase () =

( ) exp0z E iE

{Euler’s Formula}

2 2

0Re ( ) Im ( ) E Ez z E

real imaginary

► Complex representation of wave enable us to combine the

amplitude and the phase into a single function.

{Original function}

Polarization

► Polarization is described by specifying orientation of the “electric field”.

Considering x-z plane

exp E Ex 0E ikz

0 E E y

Considering y-z plane

0 E E y

exp E Ex 0E ikz

exp0

E ikzx

E

0y E

=

z

y

x

►Mie Solution to Maxwell’s Equations

(commonly known as Mie Theory)

►Mie Simulator GUI

Plane Wave Incident on a Spherical Scatterer

Plane Wave Incident on a Spherical Scatterer

Diameter < λ Diameter > λ

Uniform electric field

throughout sphere

Non-uniform electric

field throughout

sphere

Incident field

Rayleigh Limit of Mie Scattering

Hertzian Dipole

Diameter < λ

Rayleigh Limit of Mie Scattering

Parallel (p) Perpendicular (s)

Polarization

Hertzian Dipole

0

180

0

180

x-z plane y-z plane

Diameter < λ

Mie Simulator GUI Mie Simulator GUI

Mie Solution to Maxwell’s Equations (Mie Theory)

Van de Hulst, H. C., Light scattering by small particles, Dover publications (1981)

Bohren and Huffman, Absorption and Scattering of Light by Small Particles (1983)

http://www.scattport.org/index.php/light-scattering-software

2 2

21

2(2 1)

sca n n

n

Q n a bx

Scattering efficiency (Qsca)

Scattering cross section (𝜎𝑠) 2 s scaQ a

Far-field amplitude scattering matrix components

1

1

1

1

cos2 1cos

1 sin

n

n n n

n

Pn dS a b P

n n d

1

1

2

1

cos2 1cos

1 sin

n

n n n

n

Pn dS b a P

n n d

Phase function 2 2

1 2

2( )

ave

sca

S Sp

Q x

2

1

( ) 0( )

0 ( )

SS

S

Mie solution is an analytic solution to Maxwell’s equation for an incident plane wave

Mie Simulator GUI

s=Nss

g

’

Ns

s = s(1-g)

Poly disperse: Gelebart et al. Pure Appl. Opt., 5 (1996) http://virtualphotonics.codeplex.com/wikipage?title=Getting%20Started%20Mie%20Simulator%20GUI

Plane Wave Incident on a Spherical Scatterer

Detector A

• Mie solution provides 3-D scattering field

What information is necessary to

find the total electric field at point

“A”?

- Incident electric field

- Scattering field (from Mie Theory)

- Distance to point “A”

Plane Wave Incident on a Spherical Scatterer

A

HC Van de Hulst, “Light Scattering by small particles” Dover, (1981)

cos sin

sin cos 0

E E

E

i inc

i

Incident field on scattered plane

Plane Wave Incident on a Spherical Scatterer

Scattered electric field at A is given by

2

1, , exp( ) , E Es ir ikr S r

ikr

A

HC Van de Hulst, “Light Scattering by small particles” Dover, (1981)

cos sin

sin cos 0

E E

E

i inc

i

Incident field on scattered plane

1

1, , exp( ) , E Es ir ikr S r

ikr

Phase Scattering

amplitude 1/r

{Sph. Wave}

Plane Wave Incident on a Spherical Scatterer

Scattered electric field at A is given by

2

1, , exp( ) , E Es ir ikr S r

ikr

A

HC Van de Hulst, “Light Scattering by small particles” Dover, (1981)

cos sin

sin cos 0

E E

E

i inc

i

Incident field on scattered plane

1

1, , exp( ) , E Es ir ikr S r

ikr

Phase Scattering

amplitude

2

1

( , ) ( , ) 01exp( )

( , ) 0 ( , )

E E

E E

s i

s i

S rikr

S rikr

1/r

{Sph. Wave}

For non-spherical scatterers 2 3

4 1

( , , ) ( , , )

( , , ) ( , , )

S r S r

S r S r

Focused beam as a summation of plane waves

Richards and Wolf. Proc. Royal Soc. Lond. A 253(1274) (1959)

Capoglu et al. Opt. Express 16(23), (2008)

Elmaklizi et al. JBO 19(7) (2014)

Huygens-Fresnel (HF) principle: Each point of an advancing wavefront

act as a source of outgoing secondary spherical waves

HF Wavelet: A small section of

a secondary spherical wave

Airy Pattern Formation

Airy disk radius (r) (the distance between the central maximum and the

first minimum) 0.61 /r NA

Constructive interference Destructive interference

http://zeiss-campus.magnet.fsu.edu/tutorials/basics/airydiskformation/index.html

Focused Beam Propagation in Free Space

max 2

0 0

1( , , ) exp( ) ( , ) exp cos sin cos( ) sin

2

E E kf

z ikf i kz k d di

Phase at 𝜌, 𝜑, 𝑧

w.r.t. focal point

Electric field at

lens surface

Phase at the focal

point w.r.t. lens Richards and Wolf. Proc. Royal Soc. Lond. A 253(1274) 1959

Geometrical representation

Analytical solution:

max

Focused Beam Propagation in Free Space

Focused Beam Simulator GUI: Analytical solution

HF Wavelets to Model Focused Beam Propagation

Implementation in a non scattering medium

Generate uniformly distributed points (HF radiating source

locations) on the spherical cap

Project wavelets from each radiating source to a detector point

Phase advances with traveling distance

( , ) cos sin

( , ) sin cos 0

E E

E

inc ( , ) ( , ) exp( )A

jikd E E

( , ) ( , ) exp( )A

jikd E E

A

( , )

( , )

E

E

A

x y z

A

x y z

E i E j E k

E i E j E k

║and ┴ polarization calculation HF wavelet propagation Wave summation (interference)

Verifying results in a non scattering medium with the analytical solution

(A)

Analytical Solution

(B)

HF-WEFS*

Simulation parameters

: 800nm, nm:1.33, f:500m, NA:0.667

(A) – (B)

HF Wavelet based Electric Field Superposition (HF-WEFS)

*Ranasinghesagara et al, JOSA A 31(7) 2014

HF Wavelet based Electric Field Superposition (HF-WEFS)

Verifying results in a non scattering medium with the analytical solution

Detection of Scattering Fields

Primary and secondary scattering detection

HF Wavelets to Model Focus Beam Propagation

Implementation in a medium with spherical scatterers

Generate uniformly distributed points (HF radiating source

locations) in the spherical cap

Project wavelets from each radiating source to a scatterer

Phase advances with traveling distance

Find scattering angle and distance from scatterer to the detector

point

Calculate scattered field contribution at the detector from Mie

solution

( , ) cos sin

( , ) sin cos 0

E E

E

inc( , ) ( , ) exp( )

P

iikd E E

( , ) ( , ) exp( )P

iikd E E

2

1

( , ) ( , ) 01exp( )

( , ) 0 ( , )

Ps i

Ps i

S rikr

S rikr

E E

E E

( , )

( , )

A

x y z

A

x y z

E i E j E k

E i E j E k

E

E

Comparing HF-WEFS results with FDTD

Simulation parameters: : 800nm, nm:1.33, f:500m, NA:0.667

Ranasinghesagara et al, JOSA A 31(7) (2014)

A B C D

HF-WEFS

FDTD

10µm

Amplitude correlation as a function of scatterer location

Ranasinghesagara et al, JOSA A 31(7) (2014)

Axial displacement of the largest amplitude point

Ranasinghesagara et al, JOSA A 31(7) (2014)

HF-WEFS in a medium with spherical scatterers