Post on 24-May-2018
- 1 -
- BOOKLET for NEWCOMERS- GENERAL INFORMATIONS and SECURITY INSTRUCTIONS-
UMR 5557 La Doua Site
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 2 -
Welcome to the Microbial Ecology Unit (UMR 5557).
Whether you are based in one of four buildings on the Doua site (the fourth and fifth floors of the Mendel building, the first floor of the Lwoff building , the second floor of the Dubois building and the fourth floor of the Forel building), you will find in this booklet a number of relevant information concerning the people who work here, the floor plans, laboratory contact information, rules....In addition, this booklet contains essential points concerning safety in the laboratory.
These instructions concern all newcomers, this form should be read, signed and respected.
PLAN
GENERAL ORGANISATION OF THE LABORATORY p3UPON ARRIVAL TO THE LABORATORY ..... p9GENERAL RULES p10SECURITY IN THE LABORATORY p12RESPONSIBILITIES p13GOOD LABORATORY PRACTICES p15WASTES MANAGEMENT p18RESPECT RULES AND ENSURE RULES ARE RESPECTED p21WHAT TO DO UPON INCIDENT, ACCIDENT P25
SIGNALING NOTE p27SIGNATURE OF NEWCOMERS p28
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 3 -
GENERAL ORGANISATION OF THE LABORATORY
The UMR CNRS 5557 unit includes over a hundred members, researchers, lecturers, technicians, engineers, and administrative staff, including PhD students, Master 1 & 2 students, and others. These people are spread over several sites: The Doua site, the ENVL and Rockefeller sites, with extremely diverse constraints such as schedules. The UMR 5557 currently includes eight teams (see organigramme below), which are distributed over the sites mentioned above.This booklet of health and safety rules specifically concerns all users of the various buildings on the La Doua site, Université Lyon 1: Mendel, Lwoff, Dubois and Forel buildings.
UMR members who plan to work over the two other sites will need to learn about the rules of operation from the Security responsible agent (APs) of each site.
In all cases, your continued participation to the laboratory life is essential to maintain safety, comfort, harmony as well as good working conditions within the UMR.Be careful to take into account the operating constraints that apply to everyone.Do not hesitate to question the responsible persons for any situation that seems abnormal, whether this concerns you directly or not.
There is only one email address for all your enquiries concerning health and safety aspects and to report any problem (accidents, dangerous situation ...):
acmo-umr5557@listes.univ-lyon1.fr
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 4 -Distribution of the different buildings housing teams of the Ecologie Microbienne Laboratory on the La Doua site
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
Meeting
- Library
413
4°C room
37°C room
418
418ICommon Room:
Equipment +BET area
420 422
Washing room
421
Team 5PGE+Team 3
PCR room Laminar flow hood
419417415
MENDEL 4 th floor - SOUTH
Chromatography
- 5 -
Plans of the Mendel building floors
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
Radioactivity Room
22
Small meeting room
406
405 407 413
Team7
402 P2
403
406 410 412 414
Team 4
416
Team 1
MENDEL 4 th floor - NORTH
Team 5
Printers - Fax -Photocopying machines
Team 1iBio
- 6 -Building plan Mendel (5th floor – North)
(Incubating room, Microscopy room, Printers, Freezers room, Laminar flow hoods, Common machines room)
Building plan Mendel (5th floor – South)
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 7 -Lwoff and Forel buildings plans
Lwoff 1st Floor - 10 rue Raphael Dubois.
Lwoff Ground floor - 10 rue Raphael Dubois.
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 8 -
FOREL 4th Floor- 6 rue Raphael Dubois.
DUBOIS 2nd Floor- 6 rue Raphael Dubois. Administration
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 9 -
YOU HAVE JUST ARRIVED IN THE LABORATORY ..... Your tutor is directly responsible for your installation, your safety and your good integration in the laboratory, and with him you must:
• Make sure you have completed all formalities with the University and / or the CNRS and you have obtained the necessary safeguards for your activity in the laboratory, especially in case of accident (liability insurance, training agreement, etc ...),
• Get to know your colleagues, and give your name to the laboratory secretariat (Christine Delorme Tel 04.72.43.13.77 Forel Bldg)
• See Danis Abrouk to obtain an access badge to the laboratory,
• Contact the person responsible for health and safety, called ACMO in your building:Mendel Building Audrey DUBOST, AP Référent audrey.dubost@univ-lyon1.frLwoff Building Jeanne DORE, AP jeanne.dore@univ-lyon1.fr
Florence-Hélène TRAN, AP florence.tran@univ-lyon1.frForel Building Guillaume MEIFFREN, AP guillaume.meiffren@univ-lyon1.fr
Laurence LOISEAU, AP Laurence.loiseau@univ-lyon1.fr
Rockefeller Site Delphine MOUNIEE, AP delphine.mouniee@univ-lyon1.frSerge MICHALET, AP serge.michalet@univ-lyon1.fr
Vetagro sup Site Marion BOUVIER CROZIER, correspondant H&S
marion.bouviercrozier@vetagro-sup.fr
HEALTH AND SAFETY AT WORK is it available at the entrance to each floor
• Make contact, through the person who tutors you, with the person competent in radiation protection, Bruno TILLY bruno.tilly@vetagro-sup.fr if you need to handle radioisotopes.The list of documents you should receive comprises:
• Badge of the laborator• Information sheet• Internal rules• Material Safety Data Sheet• Safety Booklet .
INTERNAL RULES
In any event, all staff must work 7h42min daily for 38.5 hours weekly. Working time is "effective" working time. It does not comprise the mandatory lunch break that cannot be less than 45 minutes nor more than two hours.
HOWEVER ALL MEMBERS OF THE LABORATORY MUST BE PRESENT AND IN ACTIVITY OVER AN INCOMPRESSIBLE TIME PERIOD RANGING FROM 9:30 TO 11:30 AND FROM 14H TO 16H.
Attendance to the weekly laboratory meeting is compulsory. The meeting is held on tuesdays at 12h45, lasts for about an hour and permit transmission of information to all.
Working "alone" and working outside 'normal' hours
• It is important to know that working «alone " has no clear legal framework to date. It should remain exceptional and punctual. Should any mishap occur, the criminal responsibility of the Unit Director Unit and that of the concerned personnel is engaged.
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 10 -• For reasons of safety and liability, the laboratory director must be informed of
persons likely to come to work outside normal hours including during weekends, and he must have agreed to it.
• It is important to realize that working "alone" even if it does not cause additional risk by itself, greatly increases the time of implementation of the alert in case of emergency incident / accident.
• To date authorized personnel are:o researchers and teacher-researchers and PhD students for research purposes,o Technical personnel (ITA / IATOS) for clearly identified service-related reasons, but
never alone. All other agents who do not have a service reason should not come to the lab after hours.
• In particular, trainees (Master 1 or 2, ATER, BTS, etc.) should not be alone in the laboratory in the absence of their tutor or of an authorized person. It is the responsibility of individual tutors to ensure compliance with this rule.
Missions
All staff traveling in France or abroad in the framework of his functions for Meetings, Collaborations, Courses or Training must be in possession of a signed mission order which is thus a legally compulsory requirement. This document is mandatory in terms of administrative and legal requirements, it provides insurance coverage of the agent in case of Accidents occuring during the Service. In the absence of a signed mission order, there is no insurance coverage.
• In addition, any agent required to go directly from home to a temporary place of work without going first to his normal administrative place of work, is covered in case of accident, subject to fulfilling one of the following two conditions:- Be in possession of a signed mission order- Have a written statement by the laboratory director.
Contact:dominique.boulanger@univ-lyon1.frcompta-umr5557@listes.univ-lyon1.fr
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 11 -
SECURITY IN THE LABORATORYYou are in a research laboratory that is also designed to accommodate students receiving teachings given at the University Lyon 1. However, if the rules above apply specifically to research, some issues relate directly to teaching activities, especially in terms of overall safety in the laboratory. The internal regulations of the UMR unit are additions to the specific regulations each agent must respect in relation to his status and his parent organisation (CNRS, University, INRA, ..)
• Respect entrance and exit rules
• Do not leave your belongings unattended: bags, wallets or other.
• Ensure that laptops are secured to prevent theft in offices.
• Do not hesitate to inquire as to the whereabouts of any person you do not know.
• Unless an unusual situation arises, do not lend your laboratory badge to an outsider, please refer to your direct tutor.
• The presence of any visitor (professional or otherwise) that you take the lab is your direct responsibility. Make sure that his presence and activities within the laboratory are consistent with the general running, and do not pose a security problem (authorization to the visitor for work; presence of children in the laboratory, etc..,. ...).
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 12 -
RESPONSIBILITIESMendel building
Forel BuildingLwoff Building
Labo 123 MEIFFREN Guillaume
Evaporation room
MEIFFREN Guillaume
Open space MEIFFREN Guillaume
GPC-MS Room MEIFFREN Guillaume
LC-MS Room MEIFFREN Guillaume
Chemical wastes
MEIFFREN Guillaume
Biological wastes LOISEAU Laurence
PARMIC Room LOISEAU Laurence
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
Room 401 (Radioactivity) TILLY Bruno (PCR)
Room 402 CHAPULLIOT David / VIAL Ludovic
Room 403 MULLER Daniel / VACHERON Jordan
Room 404 (P2) BERTOLLA Franck
Room 405 (Microscopes) PUJIC Petar
Room 407 LERONDELLE Catherine
Room 409 bis ABROUK Danis
Room 413 FOURNIER Pascale
Room 416 FOURNIER Pascale
Room 419 PUJIC Petar
Room 420 SANNAIRE Corinne
Room 421 GUILLAUMAUD Nadine
Room 502 WISNIEWSKI Florence
Room 525 BROTHIER Elisabeth
Room 504 En attente de responsable
Chemical and biological wastes DUBOST Audrey
Chemical Products HAURAT Jacqueline / BROTHIER Elisabeth
Molecular Biology Kits HAURAT Jacqueline
Consummables and small supplies FOURNIER Pascale
Centrifuges and rotors PUJIC Pierre
Incubators / pH-meter à discuter
Autoclave/glassware washing machine CHAPULLIOT David
Speed Vac and its pump PRIGENT-COMBARET Claire
Proteomics PUJIC Petar
Agitation apparatus WISNIEWSKI-DYE Florence
101/103 Room TRAN Florence-Hélène
Media preparation Room FRAISSINET-TRACHET Laurence
Autoclave Room DORE Jeanne
Room 111 VALLON Laurent
Room 113 FRAISSINET-TRACHET Laurence
Room 117 LUIS Patricia
Room 122 LUIS Patricia
Chemical wastes DORE Jeanne
Biological wastes DORE Jeanne
- 13 -
GOOD LABORATORY PRACTICESCommon Protections
Common protection equipments must be used in priority. The main ones are :• fume hoods, ventilated enclosures with air depression, connected by an extractor to the outside.• portable chemical hoods, which suck in air and eject it back into the laboratory after passage
through an activated carbon filter (usually for specific volatile compounds),• protective barriers, which must be resistant and placed before each manipulation of chemicals
that have a projection or explosion hazard• the laminar flow hoods or Microbiological Safety Cabinets (MSC),• in the case of highly toxic products, weighing shall be done under the powder hood.
Individual Protections
Wearing personal protective equipment (PPE) is MANDATORY. This comprises at least:
• A cotton lab coat with long sleeves (no lab coat in offices & out of the lab),
• safety glasses with side shields,
• gloves resistant to the products handled.
Respiratory protection is, if any, provided by masks or cartridge filters adapted to the pollutants one is working with. The masks can be used in autonomous ad hoc interventions. Dust masks do not protect from chemical vapors.
TYPES OF RISKChemical risks
TraductionAll chemicals bottles have label on which are written the risks associated with this product. They generate risks by chemical toxicity, flammability, corrosivity, ...• Learn about the steps to take and the risks associated with product use,• Store used products in their original location.
Biological risks BE UP TO DATE IN YOUR VACCINATIONS !
The laboratory generates biohazards, or more precisely the risks of contamination and infection. The risk is mainly microbial.
Air is the main path of entry for biological agents, it is also the most insidious. They get in by inhaled aerosols or by accidental ingestion. Aerosols generated by centrifuges not closed or opened prior to a complete stop, or unlocked vortex tubes, are poorly quantified and their virulence is difficult to establish. Failure to comply with hygiene rules are unfortunately often sufficient to explain a number of infections : eating in the laboratory (a dining room is available), smoking (all public building are NON-SMOKING), touching your mouth with your hands without previous washing as many mistakes to avoid.
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 14 -
The mucocutaneous path has three modalities:• broken skin (cut or accidental puncture, projections on broken skin),• projection on mucous membranes,• projection on healthy skin.
Entry through the digestive tract is always due to a breakdown in personal hygiene measures (hand washing default) or to technical errors (mouth pipetting).
Radioactivity Radioactivity should be handled in room 401, that has been set aside for this purpose. When using
radioactivity, contact in advance the manager: Bruno TILLY (04 78 87 25 55 or 06 63 91 27 22 /Mendel Bldg 5th floor and VetAgroSup / bruno.tilly@vetagro-sup.fr) .
L2 (type 2 confinement laboratory) Agent in charge: Franck BERTOLLA Any student planning to work in the L2 will: be trained in advance to abide by safety rules (displayed at the entrance) always be under the responsibility of people allowed into the L2 (list at the entrance of L2).
I2 (type 2 confinement insectarium) Agent in charge: Claire VALIENTE MORO and Florence-Hélène TRAN
Any student planning to work in the I2 will: be trained in advance to abide by safety rules (displayed at the entrance) always be under the responsibility of people allowed into the I2.
Equipments under pressure
Pressure equipments are commonly used in our institution. They are used either for research or are linked to buildings safety. Lack of maintenance or improper handling can lead to an explosion, implosion or breakage of fragile items (seals, windows, regulators) and may project shards at high velocity, cause shock waves, leakage of liquid or gas. Apart from the risks related to pressure, some devices have risks that depend on the nature of the product rejected: intoxication, inflammation, explosions.
These are==> Pressure vessels• steam (autoclave for sterilization, boiler, ...)• Gas (autoclave reaction, gas cylinder, compressor and enclosure ...)• liquid==> Devices under vacuum (evaporator or lyophilizer chamber experience, ...)For obvious security reasons, only authorized persons can use autoclaves, these are :
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 15 -
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 16 -
WASTES MANAGEMENTChemical wastes
Liquid wastes
5L or 10L clear containers (only the label change)
Solid wastes
Syringes,pipettes …
Solid wastes
5, 20 or 30l Pails tips, chemically soiled Eppendorf tubes
Neofuts 70l or 35l disposable barrelsSoiled solid wastes
Fûts à ouverture totale 30 or 60l (Completely open barrels ) Glassware broken or chemically soiled
Identify containers : producing laboratory, product or type of product. Do not mix products : compatibility. Stock containers in a well ventilated room, keep away from heat, sparks or open fire.
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 17 -
Biological wastes
Barrels 30/60l Autorised = All types of biological wastes. Treatment = Incineration at very high temperature.
Cartons 23/50l Autorised = Culture media, Petri dishes, gloves, sharp consumables, conditionned in normalised packing. Treatment = Incineration or standard.
Identify the service of origin on the container. Respect the filling and weight limits of containers. All sharp objects must be conditionned in normalised packing if disposal is done in cartons. Do not store chemical wastes. Store containers in dry room with constant temperature : in the biological waste hold. Store only biological wastes in the biological waste hold.
Objects should be sorted on the lab bench by the experimenter prior to disposal.
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 18 -
Models of labels
Chemical wastes
Biological wastes
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 19 -
RESPECT RULES AND ENSURE RULES ARE RESPECTED. • Ensure that your installation and bench working conditions are known to the people likely to interact with you and that they do not disrupt existing activities.
• Organize, store and clean your bench with ethanol (70 °) after handling.
• Ensure the smooth running of the laboratory in particular at the coffee room and library.
• Allow a smooth working of the air-conditioned rooms, by closing the doors.
• Observe the prohibition of eating, drinking, smoking in the laboratory, a room is available for this.
• Wash your hands after handling.
• Learn to identify different risks from the labels : radioactive, chemical, biological, electrical, etc. risks......
• Learn how to sort and dispose of your wastes. Identify hazardous wastes.
• Learn to use the ventilation devices (fume hoods, chemical hoods)
• Make sure before you bring a visitor or non-professional in the lab that you have approval from your director. Do not let anyone outside the laboratory experiment without the agreement of your tutor. Visitors must be accompanied in experimental rooms.
• When planning a new experiment, consider prevention- Evaluate the risks- Provide the protection means- Plan for waste disposal
• Think about your colleagues and staff to service, maintain your own bench and the equipment you use (balance, centrifuge, ...).
• Because of the danger of explosion, it is forbidden to store flammable solvents in refrigerators (ether, chloroform, etc..).
• As soon as fire doors close or in case of a serious incident,Call « 30 » immediately from any telephone
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 20 -
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 21 -
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 22 -
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68
Update : May 2015
- 23 -
WHAT TO DO UPON AN INCIDENT, AN ACCIDENTAny person who witnesses an accident should call for help and indicate:• The nature of the accident,• The number of injured persons and their condition,• The location of the accident,• Additional risks,• The name and telephone number where they can be reached.If the person is a certified rescuer, provide first aid.The person has to go to the entrance of the facility to host and conduct rescuers until the accident.The Laboratory Director, the Head of Service and the ACMO will be notified.Any incident should be entered in the health and safety register.Injuries, even minor, must be registered with the medical service.All accidents must be reported within 48 hours.
TO DECLARE A WORK ACCIDENT In case of a serious incident or accident requiring a statement, you must inform the secretariat of the laboratory- Yvan MOENNE- LOCCOZ or Audrey DUBOST as soon as possible, and file in duplicate documents to the secretariat.Instructions for making an accident report are clearly detailed in the links below, for the different types of staff (University, Students, CNRS):
For the UCB Lyon 1 Universityhttp://www.univ-lyon1.fr/05124120/0/fiche___pagelibre/&RH=INT_MT-URG For the CNRShttp://www.dr7.cnrs.fr/rubrique.php3?id_rubrique=256
Some useful numbers
® Head of the Hygiene and Security Service UCB Lyon 1 Murielle PIERRE 04 72 43 18 79 murielle.pierre@univ-lyon1.fr
® Prevention and Security CNRS agentPatricia LANDAIS 04 72 44 56 07 Patricia.Landais@dr7.cnrs.fr
® Work Medecine Personnel Service UCB Lyon 1 Dr GONNAZ 04 72 44 82 11 ou 04 72 43 12 01 (Paul Dirac
bldg)CNRS Dr MICHEL or Dr DUFOURT 04 72 44 56 81
® InfirmerieUCB Lyon 1 Patricia DUCERF 04 72 43 12 02
Isabelle SABOT
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015
- 24 -Marie VANCON-MENEZ 04 72 44 82 55Great amphithaeater hall (La Doua) 04 72 43 16 22
[from monday to thursday (8–16 h), friday (8–11h45)]
CNRS Claire-Françoise VERRIES 04 72 44 56 03
® Inter University Service of Preventive Medecine 04 72 44 40 80 [from monday to thursday (8-12 h and 13-17 h), friday (8-11 h)] - Fax 04.72.44.40.90 - mail : mpu@univ-lyon1.fr
UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex
Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015