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 Aseptic Manipulations

Maulita Cut Nuria, M.Sc., Apt

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Background

Laminar Air Flow Hoods

 

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Definitions• Enteral

– Of or relating to the intestines(e.g. by mouth, orally, per tube)

• Parenteral

– Introduced other than by way of the intestinese.g. n ravenous, n ramuscuar, su cu aneous

• Aseptic Technique

 sterile products and parenteral preparations thatprevents contamination

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Definitions (cont.)

• Contamination – any effect or action that has a negative' 

– Chemical composition

–

– Sterility (e.g. microorganism contamination)

– Pyrogenicit

– Biological or therapeutic potency

– Physical appearance

– Particulate matter (e.g. dust, glass or precipitation)

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Back round

Sources of roduct contamination

• People (most common)– Touch contamination

– Generation of particulates from shedding cells or hair

• Supply air– Heating Ventilation and Air Conditioning HVAC

• Infiltration

–• Internal generation

–

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Laminar Air Flow Hoods

• The underlying principle of a laminar air flow

hood is that a constant flow of HEPA filtered airat a rate of approximately 90 linear feet per

prevents the entry of contaminated air

• The hood workspace is used to prevent thecontamination of compounded sterile products.

• HEPA filter - High Efficiency Particulate Air filter

. .larger

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Laminar Air Flow Hoods (cont.)

HEPA Filter in Frame

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Laminar Air Flow Hoods (cont.)

• Horizontal Flow Laminar Flow Hood

– Air blows towards worker

• Vertical Flow (Biological Safety Cabinet or

Chemotherapy Hood)–

protect the worker

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Laminar Air Flow Hoods (cont.)

• The HEPA filter is located in the fragile mesh between

HEPA filter screen• Nothing should be permitted to come in contact with the

HEPA filter

– NO cleaning solution– aspra e rom syrnges

– NO glass from ampules

– ,– DO NOT touch HEPA filter

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Laminar Air Flow Hoods (cont.)

• Only products essential to product preparationshould be placed in the laminar flow hood to

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Laminar Air Flow Hoods (cont.)

• Eatin drinkin and smokin is alwa s

prohibited• Talking or coughing should be directed away

rom e oo o mnmze a r ow ur uence

• A mask covering mouth and nose must be wornwhile workin in the hood 

• The use of a laminar flow hood alone without theobservance of aseptic technique, cannot insure

pro uc s er y

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 Autoclaves Autoclaves

Safe and Effective UseSafe and Effective Use

Princi les of Steam Sterilization

Sterilization: the com lete destruction of allforms of microbial life, including bacterial spores

Steam under pressure reaches hightemperatures

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Principles of Steam Steril ization (cont.)

“ ” 

heat of vaporization– 80 calories: 1 L water to boilin

– 540 calories: 1 L boiling water to steam

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 Action of Steam

,

condenses, causing a huge decrease involume and settin u a ne ativepressure that draws more steam

Condensation occurs as lon as there isa temperature differential

Action of steam ensures: Surface

heating, penetration, and proteincoagulation

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Standard Temperature and Pressure

° ° 

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How Long to Autoclave

Dr oods 121 °C  Time min

Glassware, empty, inverted 15

Instruments, wrapped 30

Utensils, wrapped 30

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Special Situations

 

– differs among media 

– beaker full of microfuge tubes with aluminum

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