LAF dan Autoclave.pdf
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Transcript of LAF dan Autoclave.pdf
7/15/2019 LAF dan Autoclave.pdf
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Aseptic Manipulations
Maulita Cut Nuria, M.Sc., Apt
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Background
Laminar Air Flow Hoods
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Definitions• Enteral
– Of or relating to the intestines(e.g. by mouth, orally, per tube)
• Parenteral
– Introduced other than by way of the intestinese.g. n ravenous, n ramuscuar, su cu aneous
• Aseptic Technique
sterile products and parenteral preparations thatprevents contamination
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Definitions (cont.)
• Contamination – any effect or action that has a negative'
– Chemical composition
–
– Sterility (e.g. microorganism contamination)
– Pyrogenicit
– Biological or therapeutic potency
– Physical appearance
– Particulate matter (e.g. dust, glass or precipitation)
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Back round
Sources of roduct contamination
• People (most common)– Touch contamination
– Generation of particulates from shedding cells or hair
• Supply air– Heating Ventilation and Air Conditioning HVAC
• Infiltration
–• Internal generation
–
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Laminar Air Flow Hoods
• The underlying principle of a laminar air flow
hood is that a constant flow of HEPA filtered airat a rate of approximately 90 linear feet per
prevents the entry of contaminated air
• The hood workspace is used to prevent thecontamination of compounded sterile products.
• HEPA filter - High Efficiency Particulate Air filter
. .larger
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Laminar Air Flow Hoods (cont.)
HEPA Filter in Frame
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Laminar Air Flow Hoods (cont.)
• Horizontal Flow Laminar Flow Hood
– Air blows towards worker
• Vertical Flow (Biological Safety Cabinet or
Chemotherapy Hood)–
protect the worker
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Laminar Air Flow Hoods (cont.)
• The HEPA filter is located in the fragile mesh between
HEPA filter screen• Nothing should be permitted to come in contact with the
HEPA filter
– NO cleaning solution– aspra e rom syrnges
– NO glass from ampules
– ,– DO NOT touch HEPA filter
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Laminar Air Flow Hoods (cont.)
• Only products essential to product preparationshould be placed in the laminar flow hood to
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Laminar Air Flow Hoods (cont.)
• Eatin drinkin and smokin is alwa s
prohibited• Talking or coughing should be directed away
rom e oo o mnmze a r ow ur uence
• A mask covering mouth and nose must be wornwhile workin in the hood
• The use of a laminar flow hood alone without theobservance of aseptic technique, cannot insure
pro uc s er y
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Autoclaves Autoclaves
Safe and Effective UseSafe and Effective Use
Princi les of Steam Sterilization
Sterilization: the com lete destruction of allforms of microbial life, including bacterial spores
Steam under pressure reaches hightemperatures
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Principles of Steam Steril ization (cont.)
“ ”
heat of vaporization– 80 calories: 1 L water to boilin
– 540 calories: 1 L boiling water to steam
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Action of Steam
,
condenses, causing a huge decrease involume and settin u a ne ativepressure that draws more steam
Condensation occurs as lon as there isa temperature differential
Action of steam ensures: Surface
heating, penetration, and proteincoagulation
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Standard Temperature and Pressure
° °
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How Long to Autoclave
Dr oods 121 °C Time min
Glassware, empty, inverted 15
Instruments, wrapped 30
Utensils, wrapped 30
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Special Situations
– differs among media
– beaker full of microfuge tubes with aluminum
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