Image-Pro PremierBasic Training Course

Part 4 - Calibration and Measurements

Measuring Objects

Groups of pixels together make objects

From Outlines we can get measurements:

Perimeter

Area

Bounding box

Centre coordinates

Radii

Much, much more...

Calibrations

Calibrations make sense of measurement

Transforms distances from Pixels to real world units

All images must be calibrated for spatial measurements

40µm

Creating Calibrations

Use known values to estimate

•Cal = Sampling Rate

Instrument Mag

Or, take image of reference object

Creating Calibration

1.Take image of known sample

2.Go to Capture Ribbon

3.Select Quick Calibration tool

4.Drag line over known distance

5.Select units and qualify line

Calibrating Images

From Capture Ribbon

Select Calibration from Characteristics box

New automatically assume current charactristics calibrations

Use green tick mark next to Spatial drop-down for image with no or incorrect calibration

Scale Bars

Calibrations enable Scale Bars and Measurements

Enable Scale Bars from Calibration group

Use Auto Marker to apply markers to every image

Use Calibration drop-down to access Marker properties

1mm1mm1mm1mm

Manual Measurements

Use Measure ribbon for manual measurements

Use Types button to select what values to collect

Use drop-downs for additional tools (e.g. straight line Vs poly-line)

Display Option

Use Options button to change:

Outline styles

Label option

Display Option

Show statistics in Measurement table

Use groupings to arrange object data by class

Change grouping options to display class statistics

Exporting Data

Export Images using:

Quick Save in Capture ribbon

Share ribbon

Export any data table to Excel, Text files or Clipboard

Measurements

Manual Measurements are a powerful tool for getting information from an image but...

...Manual measurements are also:

slow & tedious

open to operator bias