Glycopeptide Analysis (LC-MS)Glycopeptide Analysis (LC-MS)

Marker Screening by T-Test, MFC, and ROCMarker Screening by T-Test, MFC, and ROC

Protein and Sugar Chain IdentificationProtein and Sugar Chain Identification

Glyco Feature AnalysisGlyco Feature Analysis

Serum Glycoproteins Trypsin DigestionGlycopeptide Concentration LC-MS Profiling

A)

B)

C)

Number of glycopeptide peaks

Number of glycopeptide peaks

Number of glycopeptide peaks

AU

C o

f R

OC

p-va

lue

of

t-te

st

mea

n fo

ld c

han

ge

AUC=0.89

HCC vs HBV

HCC HBV HCV Healthy

20

HCC vs HCV

AUC=0.86

HCC vs Healthy

AUC=0.95

MS spectrum

MS/MS spectrum

Outer fucose

Outer fucose

SVQEIQATFFYFTPNKTEDTIFLR

AUC=0.93

HCC vs HBV

HCC HBV HCV Healthy

HCC vs HCV

AUC=0.86

HCC vs Healthy

AUC=0.95

6.5