Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain...
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Transcript of Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain...
![Page 1: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/1.jpg)
Glycopeptide Analysis (LC-MS)Glycopeptide Analysis (LC-MS)
Marker Screening by T-Test, MFC, and ROCMarker Screening by T-Test, MFC, and ROC
Protein and Sugar Chain IdentificationProtein and Sugar Chain Identification
Glyco Feature AnalysisGlyco Feature Analysis
Serum Glycoproteins Trypsin DigestionGlycopeptide Concentration LC-MS Profiling
![Page 2: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/2.jpg)
A)
B)
C)
Number of glycopeptide peaks
Number of glycopeptide peaks
Number of glycopeptide peaks
AU
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![Page 3: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/3.jpg)
AUC=0.89
HCC vs HBV
HCC HBV HCV Healthy
20
HCC vs HCV
AUC=0.86
HCC vs Healthy
AUC=0.95
![Page 4: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/4.jpg)
MS spectrum
MS/MS spectrum
Outer fucose
Outer fucose
SVQEIQATFFYFTPNKTEDTIFLR
![Page 5: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/5.jpg)
![Page 6: Glycopeptide Analysis (LC-MS) Marker Screening by T-Test, MFC, and ROC Protein and Sugar Chain Identification Glyco Feature Analysis Serum Glycoproteins.](https://reader036.fdocuments.in/reader036/viewer/2022083005/56649f2a5503460f94c43edf/html5/thumbnails/6.jpg)
AUC=0.93
HCC vs HBV
HCC HBV HCV Healthy
HCC vs HCV
AUC=0.86
HCC vs Healthy
AUC=0.95
6.5