Post on 22-May-2020
[ 3100 MASS DETECTOR ]
get mass.without massive work.
[ 3100 MASS DETECTOR ]
Stringent regulations, increasing demands for higher throughput,
and the necessity to generate the best possible data
all add to the burden of today’s analytical laboratories.
The Waters® 3100 Mass Detector provides your laboratory with
a fast, simple, and flexible analytical platform that enables
you to rapidly screen and confirm molecular weights, effortlessly
track chromatographic peaks, purify with maximum
recovery, and quantitate with superior sensitivity and selectivity.
Designed to enhance your HPLC capabilities, its rapid scan
speeds and ultra-fast polarity switching allows you to generate
higher quality information about your sample in less time,
whatever your application. The 3100 Mass Detector simply puts
advanced knowledge of your compound at your fingertips
and delivers new levels of productivity to your laboratory.
The 3100 Mass Detector provides you with all the advantages of MS detection. This versatile and easy-to-use single quadrupole MS detector allows for chemists with all levels of experience to generate superior quality results.
[ 3100 MASS DETECTOR ]
EaSily uSE MS DETEcTion – wiThouT worrying abouT MS SETup
The 3100 Mass Detector brings straightforward mass spectrometry to your laboratory benchtop, in an instrument that’s simple to operate.
This detector enables you to increase productivity through high quality results in less time and obtain advanced knowledge of your compound
with an easy-to-use platform.
an MS inST ruMEnT T haT ’S rEaDy whEn you arE
The 3100 Mass Detector uses IntelliStart Technology to automate
start-up tasks, compound optimization, MS method development,
and system performance checks. Support services available through
Connections INSIGHT ISDP are proactive, minimizing the time
needed to resolve any problems that may occur.
intelliStart Technology
IntelliStart Technology comprises both hardware and software
advancements that enable even your most inexperienced chemist to
operate the instrument quickly and produce high-quality data with
complete confidence.
With the functionality of IntelliStart, you can seamlessly set up
and operate the MS detector:
n System monitoring – Continuously monitors instrument and
system parameters in the background, ensuring your system is
ready to run.
n Resolution and calibration setup – Automatically performs
mass calibration and sets MS resolution – so you don’t have to.
n Sample tuning and MS method development – Achieves
the very best detection limits by automatically optimizing API
source conditions for specific analytical conditions and generat-
ing compound-specific Single Ion Recording (SIR) methods.
n LC/MS system check – Checks the performance of your LC/MS
system to provide analytical assurance.
connections inSighT iSDp
Use of the proactive Connections INSIGHT® Intelligent Service
Delivery Platform (ISDP) dramatically enhances your service and
support experience and provides your laboratory with a system that
enjoys maximum system uptime, increased laboratory productivity,
and lower support operating costs.
rEliabil iT y anD v ErSaT il iT y
For excellent sensitivity and selectivity, the 3100 Mass Detector
features the patented, dual-orthogonal ZSpray Atmospheric
Pressure Ionization (API) Source interface, which provides the
industry standard for salt tolerance and robustness to withstand all
types of complex matrices.
Tried and tested over several generations of Waters mass spectrom-
eters, its dual-orthogonal ion pathway ensures that fewer non-ionic
species enter the instrument, delivering excellent spectral quality
while keeping the ion optics clean. This allows you to spend more
time analyzing samples, and less time cleaning your system.
With the latest-generation ZSpray Ion Source supplied on the 3100
Mass Detector, removing and cleaning the sample cone when neces-
sary is an easy job. With the vacuum isolation valve and tool free
cone removal and replacement features, you can be acquiring data
after cleaning in almost no time at all.
Able to support ESI, IonSABRE™ APCI, ESCi multi-mode , APPI,
and dual APPI/APCI ionization modes, and combined with the latest
generation ZSpray Ion Source, the 3100 Mass Detector is one of
the most robust and flexible single quadrupole mass detectors
on the market.
[ 3100 MASS DETECTOR ]
FlExibil iT y To MEET all your analyT ical challEngES
impurity profiling
HPLC/MS with ESCi for polarity and ion-mode switching enables
your laboratory to achieve accurate and precise detection and
quantification of sample impurities in just one analytical run.
The 3100 Mass Detector’s high scan rates produce data that allows
you to confirm the identity of your compounds without the need
to outsource your analytical work to another facility – saving time
and money in high-volume laboratory environments.
peak tracking
With any method development activity, your chromatographic goal
is to successfully separate key compounds as quickly as possible
while maintaining data quality. With the 3100 Mass Detector, you
gain an easy way to monitor peak retention while HPLC/MS condi-
tions are being optimized. Peak tracking using mass removes the
need to individually inject your standard compounds. This technique
significantly increases the throughput of your method development
workflow and overall productivity.
The 3100 Mass Detector brings unparalleled sensitivity, selectivity,
and throughput in a compliant-ready platform for a diverse range
of applications.
cleaning validation
Streamline your workflow for cleaning validation. Now you can use
a single HPLC/MS method to detect multiple active pharmaceutical
ingredients while maintaining good sensitivity and resolution. Using
the 3100 Mass Detector with optional OpenLynx™ Open Access
Application Manager software enables you to create a limited num-
ber of easily-selectable methods to cover all of your formulation
analyses. This user-friendly system configuration increases labora-
tory productivity by allowing even novice chemists to simply walk
up to the instrument, choose a method that matches their sample
analysis needs, and retrieve results with minimal training.
Figure 1. Chromatogram A is a 250nm UV chromatogram of a six component mixture, run at pH3 on a Waters Sunfire C18 column, chromatogram B is composed of overlaid extracted mass chromatograms of the individual component masses from the same run. Chromatogram C is a 250nm UV chromatogram of the same six component mixture run at pH10 on a Waters XBridge C18 column, chromatogram D is composed of overlaid extracted mass chromatograms of the individual component masses from the same run.
using the waters 3100 Mass Detector allows you to effortlessly track your peaks while optimizing method conditions, which enables you to avoid running injections of individual standard compounds. This approach can significantly shorten the time it takes to develop robust and reliable methods, streamlining your workflows and enhancing your laboratory efficiency.
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b
D
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2
3
4
5
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3
4
5
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[ 3100 MASS DETECTOR ]
EaSy coluMn SwiTching
The system enables you to switch up to five columns (three
analytical and two preparative) with a single command. The
system’s fluidics layout, enhanced software features, and hardware
capabilities, combined with Waters’ proprietary Optimum Bed Density
(OBD™) Preparative Column Technology,** deliver simplified
operation.
opEn acc ESS puriF icaT ion
OpenLynx Open Access Software enables novice and experienced
analysts to utilize the full potential of the purification system with
minimal training. Chemists can literally walk up to the instrument,
submit samples through an easy-to-follow wizard or a single-page
login, walk away, and retrieve results online at their convenience.
Results can be automatically emailed or printed in a customized
format, or saved as a PDF, and data can be transferred to remote
computers. System management is just as simple. Online, an
administrator remotely defines system users and their privileges
for operating instruments across the network.
SupErior puriF icaT ion
By using mass detection to trigger fraction collection, you need
only collect your compound of interest. This makes the tasks of
fraction analysis, pooling, dry-down, and reconstitution simpler
and faster. You benefit from increased productivity in purification
activities while collecting maximum yields of your compound(s).
aDvanc ED FEaTurES
When combined with other advanced system features such as
At-Column Dilution, automated solvent management, solvent
monitoring, and remote status monitoring, the AutoPurification
System provides a fully-featured and flexible platform to cover
all your purification needs, from metabolite and impurity isolation
to biomolecule and standard purification.
Figure 2. Chromatogram A shows the Total Absorbance Chromatogram (TAC) of a seven component mixture by PDA detection. Triggering by UV detection resulted in seven collected fractions. Chromatogram B shows the MS Total Ion Chromatogram (TIC) of the same seven component mixture. Because fraction collection was automatically triggered only on the mass of interest, just one fraction was collected that contained the compound of interest.
* The Waters AutoPurification System is only available on MassLynx Software with the FractionLynx™ Application Manager. **UK patent #GB2408469
a: Tac
and represent collected fractions
b: Tic
Streamlining your purification workflow is simple… Many compo-nents of a mixture respond similarly in a uv detector and provide several fractions. The 3100 Mass Detector reduces the number of fractions collected to improve the overall efficiency of your purification process. when used together, mass spectrometry and pDa detection ensure maximum overall system efficiency.
ST rEaMlining your puriF icaT ion workFlow
The Waters AutoPurification System,* incorporating the 3100 Mass Detector, is designed for everyday mass-directed purification applications.
[ 3100 MASS DETECTOR ]
It’s accessible. With IntelliStart™ Technology, you can be certain that your system is ready
to run. Combined with an integrated fluidics system, IntelliStart Technology automates and optimizes
all instrument setup tasks, eliminating the need for manual re-plumbing, and allowing even novice
users to generate exceptional data all of the time.
It’s robust. No matter how complex (or dirty!) your sample matrix might be, safeguards built into
the 3100 Mass Detector’s ZSpray™ dual-orthogonal ionization source make it robust and reliable for daily
use. Tool-free access to the source and tool-free removal of the sample cone make it easy to clean –
within minutes – when necessary.
It’s versatile. The 3100 Mass Detector features Waters’ most popular MS technology: ESCi®
multi-mode ionization, offering both ESI and APCI as well as positive and negative switching all in one
source. The detector provides a comprehensive set of ionization modes suitable for ionization of a wide
range of compound classes with an optional APPI source and multi-mode APPI/APCI source.
It’s highly compatible. Whether your laboratory operates on MassLynx™ or Empower™
2 software, the 3100 Mass Detector easily integrates with your existing laboratory infrastructure. The
detector is also compatible with systems containing other detection technologies, including Tuneable UV
(TUV), Photodiode Array (PDA), Evaporative Light Scattering (ELS), and Fluorescence (FLR).
It’s flexible. The 3100 Mass Detector brings speed and flexibility to your applications, no matter how
diverse. Whether you need to streamline method development, get information about synthetic reactions
to chemists quicker and easier, or purify milligram to gram amounts of compound, this detector will
upgrade your laboratory capabilities to where you need them to be.
It’s smart. With a remote intelligent support system making sure your instrument is
performing at its best, laboratory downtime is minimized and problem resolution is faster with the
Connections INSIGHT ISDP.
It’s compact. And it fits on your bench. At only 34.5 cm (13.6 in.) wide, the 3100
Mass Detector is ideal for space-conscious laboratories.
Waters, ESCi, and Connections INSIGHT are registered trademarks of Waters Corporation. The Science of What’s Possible, IntelliStart, ZSpray, MassLynx, Empower, IonSABRE, OpenLynx, AutoPurification, OBD, and FractionLynx are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2008 Waters Corporation. Printed in the U.S.A. December 2008 720002815EN TK-CP
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