[ 3100 MASS DETECTOR ]

get mass.without massive work.

[ 3100 MASS DETECTOR ]

Stringent regulations, increasing demands for higher throughput,

and the necessity to generate the best possible data

all add to the burden of today’s analytical laboratories.

The Waters® 3100 Mass Detector provides your laboratory with

a fast, simple, and flexible analytical platform that enables

you to rapidly screen and confirm molecular weights, effortlessly

track chromatographic peaks, purify with maximum

recovery, and quantitate with superior sensitivity and selectivity.

Designed to enhance your HPLC capabilities, its rapid scan

speeds and ultra-fast polarity switching allows you to generate

higher quality information about your sample in less time,

whatever your application. The 3100 Mass Detector simply puts

advanced knowledge of your compound at your fingertips

and delivers new levels of productivity to your laboratory.

The 3100 Mass Detector provides you with all the advantages of MS detection. This versatile and easy-to-use single quadrupole MS detector allows for chemists with all levels of experience to generate superior quality results.

[ 3100 MASS DETECTOR ]

EaSily uSE MS DETEcTion – wiThouT worrying abouT MS SETup

The 3100 Mass Detector brings straightforward mass spectrometry to your laboratory benchtop, in an instrument that’s simple to operate.

This detector enables you to increase productivity through high quality results in less time and obtain advanced knowledge of your compound

with an easy-to-use platform.

an MS inST ruMEnT T haT ’S rEaDy whEn you arE

The 3100 Mass Detector uses IntelliStart Technology to automate

start-up tasks, compound optimization, MS method development,

and system performance checks. Support services available through

Connections INSIGHT ISDP are proactive, minimizing the time

needed to resolve any problems that may occur.

intelliStart Technology

IntelliStart Technology comprises both hardware and software

advancements that enable even your most inexperienced chemist to

operate the instrument quickly and produce high-quality data with

complete confidence.

With the functionality of IntelliStart, you can seamlessly set up

and operate the MS detector:

n System monitoring – Continuously monitors instrument and

system parameters in the background, ensuring your system is

ready to run.

n Resolution and calibration setup – Automatically performs

mass calibration and sets MS resolution – so you don’t have to.

n Sample tuning and MS method development – Achieves

the very best detection limits by automatically optimizing API

source conditions for specific analytical conditions and generat-

ing compound-specific Single Ion Recording (SIR) methods.

n LC/MS system check – Checks the performance of your LC/MS

system to provide analytical assurance.

connections inSighT iSDp

Use of the proactive Connections INSIGHT® Intelligent Service

Delivery Platform (ISDP) dramatically enhances your service and

support experience and provides your laboratory with a system that

enjoys maximum system uptime, increased laboratory productivity,

and lower support operating costs.

rEliabil iT y anD v ErSaT il iT y

For excellent sensitivity and selectivity, the 3100 Mass Detector

features the patented, dual-orthogonal ZSpray Atmospheric

Pressure Ionization (API) Source interface, which provides the

industry standard for salt tolerance and robustness to withstand all

types of complex matrices.

Tried and tested over several generations of Waters mass spectrom-

eters, its dual-orthogonal ion pathway ensures that fewer non-ionic

species enter the instrument, delivering excellent spectral quality

while keeping the ion optics clean. This allows you to spend more

time analyzing samples, and less time cleaning your system.

With the latest-generation ZSpray Ion Source supplied on the 3100

Mass Detector, removing and cleaning the sample cone when neces-

sary is an easy job. With the vacuum isolation valve and tool free

cone removal and replacement features, you can be acquiring data

after cleaning in almost no time at all.

Able to support ESI, IonSABRE™ APCI, ESCi multi-mode , APPI,

and dual APPI/APCI ionization modes, and combined with the latest

generation ZSpray Ion Source, the 3100 Mass Detector is one of

the most robust and flexible single quadrupole mass detectors

on the market.

[ 3100 MASS DETECTOR ]

FlExibil iT y To MEET all your analyT ical challEngES

impurity profiling

HPLC/MS with ESCi for polarity and ion-mode switching enables

your laboratory to achieve accurate and precise detection and

quantification of sample impurities in just one analytical run.

The 3100 Mass Detector’s high scan rates produce data that allows

you to confirm the identity of your compounds without the need

to outsource your analytical work to another facility – saving time

and money in high-volume laboratory environments.

peak tracking

With any method development activity, your chromatographic goal

is to successfully separate key compounds as quickly as possible

while maintaining data quality. With the 3100 Mass Detector, you

gain an easy way to monitor peak retention while HPLC/MS condi-

tions are being optimized. Peak tracking using mass removes the

need to individually inject your standard compounds. This technique

significantly increases the throughput of your method development

workflow and overall productivity.

The 3100 Mass Detector brings unparalleled sensitivity, selectivity,

and throughput in a compliant-ready platform for a diverse range

of applications.

cleaning validation

Streamline your workflow for cleaning validation. Now you can use

a single HPLC/MS method to detect multiple active pharmaceutical

ingredients while maintaining good sensitivity and resolution. Using

the 3100 Mass Detector with optional OpenLynx™ Open Access

Application Manager software enables you to create a limited num-

ber of easily-selectable methods to cover all of your formulation

analyses. This user-friendly system configuration increases labora-

tory productivity by allowing even novice chemists to simply walk

up to the instrument, choose a method that matches their sample

analysis needs, and retrieve results with minimal training.

Figure 1. Chromatogram A is a 250nm UV chromatogram of a six component mixture, run at pH3 on a Waters Sunfire C18 column, chromatogram B is composed of overlaid extracted mass chromatograms of the individual component masses from the same run. Chromatogram C is a 250nm UV chromatogram of the same six component mixture run at pH10 on a Waters XBridge C18 column, chromatogram D is composed of overlaid extracted mass chromatograms of the individual component masses from the same run.

using the waters 3100 Mass Detector allows you to effortlessly track your peaks while optimizing method conditions, which enables you to avoid running injections of individual standard compounds. This approach can significantly shorten the time it takes to develop robust and reliable methods, streamlining your workflows and enhancing your laboratory efficiency.

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[ 3100 MASS DETECTOR ]

EaSy coluMn SwiTching

The system enables you to switch up to five columns (three

analytical and two preparative) with a single command. The

system’s fluidics layout, enhanced software features, and hardware

capabilities, combined with Waters’ proprietary Optimum Bed Density

(OBD™) Preparative Column Technology,** deliver simplified

operation.

opEn acc ESS puriF icaT ion

OpenLynx Open Access Software enables novice and experienced

analysts to utilize the full potential of the purification system with

minimal training. Chemists can literally walk up to the instrument,

submit samples through an easy-to-follow wizard or a single-page

login, walk away, and retrieve results online at their convenience.

Results can be automatically emailed or printed in a customized

format, or saved as a PDF, and data can be transferred to remote

computers. System management is just as simple. Online, an

administrator remotely defines system users and their privileges

for operating instruments across the network.

SupErior puriF icaT ion

By using mass detection to trigger fraction collection, you need

only collect your compound of interest. This makes the tasks of

fraction analysis, pooling, dry-down, and reconstitution simpler

and faster. You benefit from increased productivity in purification

activities while collecting maximum yields of your compound(s).

aDvanc ED FEaTurES

When combined with other advanced system features such as

At-Column Dilution, automated solvent management, solvent

monitoring, and remote status monitoring, the AutoPurification

System provides a fully-featured and flexible platform to cover

all your purification needs, from metabolite and impurity isolation

to biomolecule and standard purification.

Figure 2. Chromatogram A shows the Total Absorbance Chromatogram (TAC) of a seven component mixture by PDA detection. Triggering by UV detection resulted in seven collected fractions. Chromatogram B shows the MS Total Ion Chromatogram (TIC) of the same seven component mixture. Because fraction collection was automatically triggered only on the mass of interest, just one fraction was collected that contained the compound of interest.

* The Waters AutoPurification System is only available on MassLynx Software with the FractionLynx™ Application Manager. **UK patent #GB2408469

a: Tac

and represent collected fractions

b: Tic

Streamlining your purification workflow is simple… Many compo-nents of a mixture respond similarly in a uv detector and provide several fractions. The 3100 Mass Detector reduces the number of fractions collected to improve the overall efficiency of your purification process. when used together, mass spectrometry and pDa detection ensure maximum overall system efficiency.

ST rEaMlining your puriF icaT ion workFlow

The Waters AutoPurification System,* incorporating the 3100 Mass Detector, is designed for everyday mass-directed purification applications.

[ 3100 MASS DETECTOR ]

It’s accessible. With IntelliStart™ Technology, you can be certain that your system is ready

to run. Combined with an integrated fluidics system, IntelliStart Technology automates and optimizes

all instrument setup tasks, eliminating the need for manual re-plumbing, and allowing even novice

users to generate exceptional data all of the time.

It’s robust. No matter how complex (or dirty!) your sample matrix might be, safeguards built into

the 3100 Mass Detector’s ZSpray™ dual-orthogonal ionization source make it robust and reliable for daily

use. Tool-free access to the source and tool-free removal of the sample cone make it easy to clean –

within minutes – when necessary.

It’s versatile. The 3100 Mass Detector features Waters’ most popular MS technology: ESCi®

multi-mode ionization, offering both ESI and APCI as well as positive and negative switching all in one

source. The detector provides a comprehensive set of ionization modes suitable for ionization of a wide

range of compound classes with an optional APPI source and multi-mode APPI/APCI source.

It’s highly compatible. Whether your laboratory operates on MassLynx™ or Empower™

2 software, the 3100 Mass Detector easily integrates with your existing laboratory infrastructure. The

detector is also compatible with systems containing other detection technologies, including Tuneable UV

(TUV), Photodiode Array (PDA), Evaporative Light Scattering (ELS), and Fluorescence (FLR).

It’s flexible. The 3100 Mass Detector brings speed and flexibility to your applications, no matter how

diverse. Whether you need to streamline method development, get information about synthetic reactions

to chemists quicker and easier, or purify milligram to gram amounts of compound, this detector will

upgrade your laboratory capabilities to where you need them to be.

It’s smart. With a remote intelligent support system making sure your instrument is

performing at its best, laboratory downtime is minimized and problem resolution is faster with the

Connections INSIGHT ISDP.

It’s compact. And it fits on your bench. At only 34.5 cm (13.6 in.) wide, the 3100

Mass Detector is ideal for space-conscious laboratories.

Waters, ESCi, and Connections INSIGHT are registered trademarks of Waters Corporation. The Science of What’s Possible, IntelliStart, ZSpray, MassLynx, Empower, IonSABRE, OpenLynx, AutoPurification, OBD, and FractionLynx are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2008 Waters Corporation. Printed in the U.S.A. December 2008 720002815EN TK-CP

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