Flowcytometry Workshop - Tanta Universityorl.tanta.edu.eg/cecr/Research Team/ICBS Workshop.pdf ·...

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Transcript of Flowcytometry Workshop - Tanta Universityorl.tanta.edu.eg/cecr/Research Team/ICBS Workshop.pdf ·...

باإلشتراك مع

Under the Patronage of

Prof. Ashraf El-ShihyMinister of Higher Education

Prof. Ibrahim SalemVice President of Tanta University

Acting President of Tanta University

Honory Chairman Prof. Tarek M. Fayed, Dean of Faculty of Science

Chairmen Prof. Mohamed H. Mona, President of ICBSProf. Khadiga Gaafar, President of EGSZ

Chair, Scienti�c and Organizing CommitteeProf. Mohamed L. Salem, Prof. of Immunology, Faculty of Science

Tanta University,9-10 November,

2016

املؤمتر الدوىل الثامن للعلوم البيولوجيةاملؤمتر الدوىل 21 للجمعية املصرية األملانية لعلم احليوان

THE 8th International Conference on Biological Sciences

in Collaboration with 21st International Conference of Egyptian German Society of Zoology

T A N T A U N I V E S I T YF A C U L T Y O F S C I E N C E

Faculty of ScienceTANTA UNIVERSITY

E G Y P T

THE INTERNATION

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ناجلمعية املصرية األملانية لعلم احليوا

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Under PatronageProf. Ibrahim SalemPresident, Tanta University

Prof. Amgad Abdel-RaoofDean, Faculty of Medicine

Prof. Tarek FayedDean, Faculty of Science

Prof. Gamal MousaDirector, Tanta University Educational Hospital

LecturersProf. Mohamed Labib Salem, PhDProf. of Immunology, Tanta UniversityExecutive Director, CECR

Prof. Mohamed Attia Saad, PhDAssistant Prof. of Clinical Pathology, Tanta UniversityDirector, Flow Cytometry Unit, CECR

Dr. Abdel Aziz Awad Zidan, PhDLecturer of Immunology, Damanhur UniversityDirector, Genomic & Proteomic Unit, CECR

ModeratorsProf. Said Hammad Abdou, MDProf. of Clinical Pathology, Tanta UniversityLab Director, CECR

Prof. Mohamed Ramadan Elshanshory, MDProf. of Hematology & Oncology, Tanta UniversityClinical Director, CECR

Workshop Topics• Basics of Flow Cytometry technology and how does it work• Fluorescene and �uorochromes• Flow Cytometric applications in cell proliferation apoptosis,

DNA content and cell cycle• Advantages, disadvantages, limitations and opportunities• Intensive hands-on Training

RegistrationRegistration Fees: 500 L.EIncluding Printed Materials, Lunch Break and Certi�catesCECR is a project funded by Science & Technology Development Fund (STDF) Flowcytometry

Workshop

Opening and Registration

T H E O R E T I C A L S E S S I O N

Introduction: Basics of �ow cytometry and how does the machine workProf. Mohamed Labib Salem, Director, CECR

Machine and its instrumentations (Fluidic-Optic-Electronic)Prof. Mohamed Attia Saad, Flow Cytometry Unit Director, CECR

C o f f e e B r e a k

Principles of hand on processing of samplesMs. Mona Zidan, MSc,Research and teaching Assistant, CECR

P R A C T I C A L S E S S I O N

Surface Staining: Processing of peripheral blood sampleMs. Mona F. Zidan (GP A) Ms. Sohaila Galal (GP B)Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR

L u n c h B r e a k

Samples AcquisitionMs. Mona F. Zidan, Research and Teaching Assistant, Flow Cytometry Unit, CECR

09:00 - 09:30 AM

09:45 - 10:30 AM

09:45- 10:30 AM

10:45 - 11:15 AM

11:15 - 11:45 AM

12:15 - 02:00 PM

12:15 - 02:00 PM

02:00 - 02:45 PM

02:45 - 03:45 AM

11:45 - 12:15 AM

W O R K S H O P P R O G R A MD A Y 1 : 9 t h N O V E M B E R , 2 0 1 6

Opening and Registration

T H E O R E T I C A L S E S S I O NIntroduction of applications of Flow cytometry applications in research and DiagnosisProf. Mohamed Attia Saad, Flow Cytometry Unit Director, CECR

Flow Cytometric quantitation of DNA content and cell cycleDr. Abdel-Aziz Awad Zidan, Director of Genomics & Proteomics Unit, CECR

C o f f e e B r e a k

Preparation of PBMCsMr. Sohaila Galal, Assistant Lecturer, Tanta University

P R A C T I C A L S E S S I O NIsolation of PBMCs by FicollMs. Mona F. Zidan (GP A) Ms. Sohaila Galal (GP B) Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR

Samples Staining by (Annexin V) for detection of apoptosisMs. Sohaila Galal (GP A) Ms. Mona F. Zidan (GP B) Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR

L u n c h B r e a k

Sample AcquisitionMs. Mona F. Zidan, Research and Teaching Assistant, Flow Cytometry Unit, CECR

Summary, Evaluation and Certi�cation

09:00 - 09:30 AM

10:00 - 10:45 AM

10:00 - 10:45 AM

11:00 - 11:45AM

11:45 - 12:00 AM

12:00 - 12:15 PM

12:30 - 01:15 PM

12:30 - 01:15 PM

01:15 - 01:45 PM

01:45 - 02:30 PM

02:30 - 03:30 PM

03:30 - 04:30 PM

W O R K S H O P P R O G R A MD A Y 2 : 1 0 t h N O V E M B E R , 2 0 1 6