Electrospinning and Additive Manufacturing: Converging

Technologies

Paul Dalton

Institute of Health and Biomedical Innovation Queensland University of Technology, AUSTRALIA

Tissue Engineering Research

Tissue Engineered Products

Valley of Death Funding: Venture Capital?, SBIR?, Corporate Partnerships? Basic Research Funding:

NIH, NSF, DOD, etc. Commercialization Funding: Large Companies, Private Equity

Insurance Reimbursed Clinical Products

Regulatory Approval

CLINICAL TRIALS:

Pre-Clinical Studies: ISO/FDA Guided Bench Tests: Large Targeted Animal Studies:

Design/Manufacturing: Design, History, Input GMP Manufacturing:

Basic In Vivo Research: Small Animal Model; Rudimentary Biologic/ Scaffold Constructs

Basic In Vitro Research: Cell Isolation; Material Synthesis

The Valley of Death

Surgeon Acceptance

Additive Manufacturing

Filament Resolution New Materials Required

Chaotic Deposition Solvent Process Cell Invasiveness

Electrospinning Writing Accurate Placement of Small Diameter Fibers

Solvent Free Existing Polymers (including clinically used)

Scaffold Fabrication for TE

Additive Manufacturing

Woodfield et al. (2005). Tissue Eng, 11, 297.

Custom Scaffolds

Probst FA et al. (2010) Handchir Mikrochir Plast Chir, 42, 369.

Electrospinning

http://www.nano.org.uk/news/nov2008/1688.gif

Solution Electrospinning

Xu et al (2004) Tissue Eng, 10, 1160

Melt Electrospinning

• Less than 1% of electrospinning literature (40-45 Publications in total)

• Melt Electrospinning for TE

– Saunders/Ratner (2 publications) – Park/Yang(1 publication) – Dalton (7 publications)

Dalton et al. (2007) Polymer, 48, 6823.

Electrospinning Writing & Layering

Brown et al. (2011) Advanced Materials, 23, 5651-57.

100µm

Melt Electrospinning Writing

100µm

Scaffolds for Skin TE

Farrugia et al. Biofabrication (submitted).

Cell/Scaffold Interactions

Farrugia et al. Biofabrication (submitted).

Writing onto Cylinders

100 μm

30° 60°45° 2 mm2 mm 2 mm

Brown et al. (2012) Biointerphases, 7, 13.

Tubular Scaffolds

Brown et al. (2012) Biointerphases, 7, 13.

Summary

Summary of Melt Electrospinning Writing • Distinct Process

• Inexpensive

• Good Resolution

• High Scaffold Porosity

• Solvent Free Process

• Clinical Potential

Ongoing Work – Surface Modification

Grafahrend et al, Nature Materials, 10, 67-73.

Collaborative Network

www.daltonlab.com

Acknowledgements Queensland University of

Technology

• Dietmar W. Hutmacher • Toby Brown • Tim Dargaville • Nicola Detta • Brooke Farrugia • Anna Slotosch • Anna Taubenberger • Laure Thibaudeau

University of Würzburg

• Jürgen Groll • Tomasz Jungst

Funding

• CSIRO • EU Project “Hydrozones”

www.daltonlab.com

Literature

• Dalton et al. (2013), Biomaterials Science, 1, 171. • Hutmacher & Dalton (2011), Chemistry: An Asian Journal,

6, 44-56. • Brown et al. (2012) Biointerphases, 7, 13. • Brown et al. (2011) Advanced Materials, 23, 5651-57. • Detta et al. (2010) Polymer International, 59, 1558-62. • Dalton et al. (2008) Biomedical Materials, 3, 034139. • Dalton et al. (2007) Polymer, 48, 6823. • Dalton et al. (2006) Biomacromolecules, 7, 686.

www.daltonlab.com