Post on 20-Aug-2020
DNA Electrophoresis
What is Gel Electrophoresis? • A technique that allows us to
compare DNA.
What is Gel Electrophoresis? • A technique that allows us to compare
DNA.
• Common uses you may be familiar with:– Identify a suspect in a
crime.– Paternity testing.
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
• DNA samples are cut by enzymes (chemical scissors that cut DNA at specific sequences
• Cut samples are ‘run’ on a gel and compared.
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
• DNA samples are cut by enzymes (chemical scissors that cut DNA at specific sequences
• Cut samples are ‘run’ on a gel and compared.
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The Standard.• Known DNA fragments added to compare to unknown
lengths • These are measured in kilobases (kb)
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Using the Standard• Known fragments
of various sizes are run together with tests
• Allows size estimation of unknown fragments.
• Small sizes run faster
Comparing DNA• Suspect test:
Comparing DNA
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Comparing DNA• Paternity test:
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Using Mapping Plasmids