electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that...
Transcript of electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that...
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DNA Electrophoresis
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What is Gel Electrophoresis? • A technique that allows us to
compare DNA.
![Page 3: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/3.jpg)
What is Gel Electrophoresis? • A technique that allows us to compare
DNA.
• Common uses you may be familiar with:– Identify a suspect in a
crime.– Paternity testing.
![Page 4: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/4.jpg)
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
![Page 5: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/5.jpg)
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
• DNA samples are cut by enzymes (chemical scissors that cut DNA at specific sequences
• Cut samples are ‘run’ on a gel and compared.
![Page 6: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/6.jpg)
General Overview• DNA is obtained & amplified to make
enough sample to test (using PCR- see tomorrow).
• DNA samples are cut by enzymes (chemical scissors that cut DNA at specific sequences
• Cut samples are ‘run’ on a gel and compared.
![Page 7: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/7.jpg)
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The Standard.• Known DNA fragments added to compare to unknown
lengths • These are measured in kilobases (kb)
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Using the Standard• Known fragments
of various sizes are run together with tests
• Allows size estimation of unknown fragments.
• Small sizes run faster
![Page 9: electrophoresis overview(not Lab) 2 Student · What is Gel Electrophoresis? • A technique that allows us to compare DNA. •Common uses you may be familiar with: – Identify a](https://reader036.fdocuments.in/reader036/viewer/2022071009/5fc6e73b841a6922131a56d9/html5/thumbnails/9.jpg)
Comparing DNA• Suspect test:
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Comparing DNA
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Comparing DNA• Paternity test:
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Using Mapping Plasmids