Post on 27-Mar-2015
Cellular response to DNA damage
Reponse Mechanisms
Tolerance of DNA damage Replicative bypass of template damage
Translesion DNA synthesis
Reversal of DNA damage Enzymatic photoreactivation
Repair of O6-alkylguanine, O4-
alkyltimidine
Ligation of DNA strand breaks
Excision of DNA damage Base Excision Repair
Nucleotide Excision Repair
Mismatch Repair
Double strand breaks ligation Single strand annealing
Recombinational Repair
Non-Homologous End Joining
La radiazione Solare può essere distinta in 3 componenti principali a seconda della lunghezza d’onda:ULTRAVIOLETTO (100-400 nanometri) 10%VISIBILE ( 400-750nm.) 45%INFRAROSSO (750-3000nm.) 45%
UV-induced damage
UV-responsive photolyases
Direct reversal (de-alkylating proteins)
Base Excision Repair
Nucleotide Excision Repair
(E.coli)
Nucleotide Excision Repair (Global Genome Repair -Humans)
Nucleotide Excision Repair(Transcription Coupled -Humans)
Common features of GGR & TCR