Cell Growth & Differentiation Vol. 10, 545-554, 1999

Inhibition of the Jun Kinase Pathway Blocks DNA Repair, Enhances p53-mediated Apoptosis and Promotes Gene Amplification

Ruth A. Gjerset, Svetlana Lebedeva, Ali Haghighi, Sally T. Turla and Dan Mercola

(1) MEFs: Mouse Embryonic Fibroblast

Two independent experiment system

(2) A lung tumor cell line derived from mice expressing an oncogenic K-Ras mutation (K-RasG12D)

p53 + or -

DNA damage (Doxorubicin; a drug used in cancer chemotherapy)

p53 + or -

cre-lox recombination system

Cre-Lox recombination involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase.