GROWTH OF MAINTAINING DIFFRENT KINDS OF CELLS IN AN

ARTIFICIAL ENVIRONMENT UNDER LABORATORCONDITIONS

- DIFFRENT CELLULAR ACTIVITIES SUCH AS

ENDOCYTOSIS,CELL MOVEMENT,CELL DIVISION ,MEMBRANE

TRAFFICKING AND MACROMOLECULAR SYNTHESIS CAN BE

STUDIED

COMMON METHOD GLASS OR PLASTIC SURFACE IMMERSED

IN NUTRIENT FLUID ALLOWS CELLS TO PROLIFERATE

A 3D cell culture grown with magnetic levitation (Image: G. Souza/N3D Biosciences)

NATURAL MEDIA :FLUIDS OBTAINED FROM

LIVING SYSTEMS FOR EG LYMPH ,BLOOD ,

SERUM AND EMBRYO HOMOGENATES

NUTRIENT MEDIA :IT IS THE MOST COMMON

MEDIA WHICH CONSIST OF CARBON, SALT

WATER AS WELL AS AMINOACID NITROGEN

MINIMAL MEDIA : MINIMUM NUTRIENTS

NEEDED FOR GROWTH WITHOUT THE

PRESCENCE OF AMINO ACIDS

SELECTIVE MEDIA :IT IS UDED TO CULTURE

SELECTIVE ORGANISM COMMONLY USED BY

MICROBIOLOGIST

MASS CULTURE

PRIMARY CULTURE

CLONAL CULTURE

MASS CULTURE : Large numbers of cells are added to a

culture dish where they attach to the bottom. Those

cells that survive will proliferate and after a number

of generations form a monolayer of cells that cover

the bottom of dish

CLONAL CULTURE : Cell is attached to a dish at some

distance from its neighbors. Surviving cells

proliferate and form a colony (a clone) whose

members are derived from same cell

SECONDARY CULTURE : Previously culture cells are

removed and transferred to a media is secondary

culture