Post on 14-Aug-2020
Copyright © 2012 Abcam, All Rights Reserved.Produced in collaboration with Sarah Barnes from the laboratory of Dr Sue Ozanne, Institute of Metabolic Science (IMS), University of Cambridge.
Cell ageing and metabolism
Discover more at abcam.com/metabolism
* Studies have shown increased lifespanwith over expression.
† Original paper demonstrated a 30%increase in lifespan with over expressingSirt1, however out breeding reduced thisto a more modest 10%.
Antioxidants Catalase*, Gpx1, Gsr, Hsp70*,Sod1*, Sod2*, Sod3, Prdx1,Prdx2, Prdx3, Txn1
Senescencep53, p21, p16
DNA damageGadd45g, Ku70, Ku80, Sirt6*
Master regulatorsSirt1†, Bach1, Nrf2
Telomere MaintenancePot1, Ku80, Trf1, Trf2
Product Applications/Tests Species Reactivity Product Code Product Applications/Tests Species Reactivity Product Code
410_12_MBS
Cell ageing and metabolism products from Abcam
Discover more at abcam.com/metabolism
Antioxidants | Antibodies
Catalase IHC-Fr, IHC-P, ICC, WB, IP, ELISA, ICC/IF Ms, Rat, Shp, Chicken, Cow, Hu ab1877
Glutathione Peroxidase 1 IHC-P, WB, ICC/IF, IHC-Fr Ms, Rat, Cow, Hu ab22604
Glutathione Reductase [EPR7238] WB, Flow Cyt Ms, Rat, Hu ab124995
Hsp70 antibody [C92F3A-5] Electron Microscopy, WB, IP, ELISA, ICC/IF, Ms, Rat, Sheep, Rab, Chicken, Guinea pig,
Flow Cyt, IHC-Fr, IHC-P Hamster, Cow, Dog, Hu, Pig, C.elegans, Carp,
Fruit fly (Drosophila melanogaster), Monkey ab47455
Superoxide Dismutase 1 ICC/IF, IHC-Fr, WB, IP, ELISA, IHC-P Ms, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Hu
Pig, X.laevis, Fruit fly (Drosophila melanogaster),
Monkey, African Green Monkey ab13498
SOD2 [9E2BD2] ICC/IF, IHC-P, IP, In-Cell ELISA, Flow Cyt Ms, Rat, Cow, Hu ab110300
Superoxide Dismutase 3 IHC-Fr, IHC-P, WB Hu ab21974
Peroxiredoxin 1 ICC/IF, IHC-P, WB, ICC Ms, Rat, Hu, African Green Monkey ab15571
Peroxiredoxin 2 antibody [EPR5154] WB, IHC-P, ICC/IF Ms, Rat, Hu ab109367
Peroxiredoxin 3 antibody [EPR8115] WB, IP, IHC-P, ICC, Flow Cyt Hu ab128953
Thioredoxin WB, ICC/IF, IHC-P Ms, Hu ab26320
Antioxidants | Kits
Catalase Human ELISA Kit 1 x 96 Hu, Rat, ms ab123456
Catalase Specific Activity Assay Kit 1 x 96 Ms, Rat, Hu ab118184
Glutathione Peroxidase Assay Kit 1 x 100 Cell culture supernatant, Urine, Serum, Plasma,
Platelets, Other biological fluids, Tissue Extracts ab102530
Glutathione Reductase (GR) Assay Kit 1 x 200 Cell culture supernatant, Urine, Serum, Plasma,
Other biological fluids, Tissue Extracts, Cell Lysate ab83461
Superoxide Dismutase Activity Assay Kit (Colorimetric) 1 x 100 Urine, Serum, Plasma, Other biological fluids,
Tissue Extracts, Cell Lysate, Cell culture media ab65354
Thioredoxin (TrxR) Reductase Assay Kit 1 x 100 Cell culture supernatant, Urine, Serum, Plasma,
Other biological fluids, Tissue Extracts ab83463
Total Antioxidant Capacity Assay Kit 1x100 Lysates, plasma, serum, urine ab65329
NAD/NADH Assay Kit 1x100 Lysates, plasma, serum, urine ab65348
NADP/NADPH Assay Kit 1x100 Lysates, plasma, serum, urine ab65349
Senescence | Antibodies
p53 [PAb 240] Flow Cyt, IHC-Fr, IP, ICC/IF, WB, IHC-P Ms, Rat, Dog, Hu ab26
p21 IHC-Fr, IP, WB, ICC/IF, ICC, Flow Cyt Ms, Rat, Dog, Hu ab7960
p16 ARC ICC/IF, Flow Cyt, IP, WB, IHC-P Ms, Rat, Dog, Hu ab51243
Senescence | Kits
p53 Human ELISA Kit 1 x 96 Hu ab117995
p53 Phospho S46 Human ELISA Kit 1 x 96 Hu ab124532
Senescence | Kits (continued)
p53 Phospho S392 Human ELISA Kit 1 x 96 Hu ab124533
p53 Total + pSer46 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab128570
p53 Total + pSer392 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab128571
Senescence Detection Kit 250 Cell culture, tissue ab65351
DNA damage | Antibodies
GADD45G WB, Flow Cyt Hu
Ku70 [N3H10] IF, IHC-P, IP, WB, ICC/IF Ms, Hu, Monkey ab3114
Ku80 [5C5] WB, ELISA, IHC-P, Flow Cyt, ICC/IF Ms, Hu ab119935
Ku70 + Ku80 [162] ICC/IF, Flow Cyt, IHC-P, IP Hu, X.laevis, Monkey ab3108
SIRT6 WB, IHC-P Hu ab118487
Anti-GAPDH + H2A.X(S139) +cleaved-PARP
antibody [3E8, 9F3 and 4B5] WB Hu ab131385
DNA damage | Kits
DNA Damage - AP sites - Assay Kit (Colorimetric) 25 Purified damaged DNA ab65353
In Situ Direct DNA Fragmentation Assay Kit
(TUNEL assay) 50 Adherent and suspension cells ab66108
In Situ BrdU-Red Fragmentation Assay Kit
(TUNEL assay) 60 Tissue, adherent cells ab66110
Master regulators | Antibodies
SIRT1 [E104] IHC-Fr, WB, ICC, IP, IHC-P Hu ab32441
BACH1/BRIP1 WB Hu ab7288
Nrf2 IHC-P, ICC/IF, IHC-Fr, WB Ms, Rat, Hu ab31163
Master regulators | Kits
SIRT1 Human ELISA Kit 1 x 96 Tissue Extracts, Cell Lysate ab123457
SIRT1 pSer47 Human ELISA Kit 1 x 96 Hu ab135392
SIRT1 Total and pSer47 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab135394
Telomere Maintenance | Antibodies
POT1 WB, ICC/IF Ms, Hu ab21382
Trf1 ChIP Grade ChIP, WB, ICC/IF, IP Ms, Hu ab1423
Trf2 IHC-P, WB, ICC, IP, ICC/IF, Flow Cyt Hu ab13579
Telomere Maintenance | Kits
EpiSeeker ChIP Kit - One Step 48 / 96 Mammalian DNA ab11713