Cell-Culture real time Monitoring system

Analog Design Contest 2013

Team: José A. Maldonado, Juan N. Olmo, Gloria Huertas, Alberto Yúfera

Cecumo

“The important thing is not to stop questioning”. Albert Einstein

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AGENDA

Motivation Theoretical background

Simulations System description

Implementation Features Results

Final conclusions Questions and comments

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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MOTIVATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Cecumo

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THEORETICAL BACKGROUND

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Z(w)/A Rs

Z(w)/(A-Ac) RsCELL

Z(w)/Ac Rgap

 

Low-Pass Behaviour function of cell-culture occupation area (Ac)

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HBP(s)

Zcell-electrode(s)

RinVoutVout2

THEORETICAL BACKGROUND: SIMULATIONS

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Oscillation-Based Test approach

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SYSTEM DESCRIPTION: IMPLEMENTATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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SYSTEM DESCRIPTION: RESULTS

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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SYSTEM DESCRIPTION: RESULTS

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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SYSTEM DESCRIPTION: FEATURES

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Real time monitoring “Cell friendly” (Max. 30mV/30μA)

Immediate result Up to 8 cultures Wireless connectivity Data logging Long battery lifetime (100mA/10mA@±5V)

Compact and light-weight design (160x80x25mm)

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CONCLUSIONS

Low cost & reusable “biological” oscillator for Cell Culture growing measurements.

Amplitude and frequency to characterize occupation. AGC for better noise inmunity Frequency and AGC control voltage (Vg) to extract Fill

Factor (ff) parameter. Potential fields of application: medicine and biology

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

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QUESTIONS AND COMMENTS

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Thank you for your attention!

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SYSTEM DESCRIPTION: IMPLEMENTATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

Band Pass Filter

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SYSTEM DESCRIPTION: IMPLEMENTATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

AGC

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SYSTEM DESCRIPTION: IMPLEMENTATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

BioZ Interface

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SYSTEM DESCRIPTION: IMPLEMENTATION

Analog Design Contest 2013 José A. Maldonado, Juan N. Olmo

High Pass Filter & Comparator