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04/11/2023 Biochemistry For Medics 1
Biochemistry Quiz- 3
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04/11/2023 Biochemistry For Medics 2
Identify the disease
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Answer
• Palmar Xanthomas
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Breath Hydrogen
• Breath Hydrogen test is done for the diagnosis of-------------------------- ?
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Answer
• Lactose intolerance
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Identify the structure and mention its use
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Answer
• Biotin• Participates in “Carboxylation reactions”
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Identify the technique and mention its use
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Answer
• DNA foot printing• For identification of promoter regions on
DNA
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Identify the instrument
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Answer
• Blood Gas Analyzer
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What is the normal reference range for CK?
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Answer
• 10-50 IU/L
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Name the enzyme for the second reaction
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Answer
• UDP –Glucuronyl Transferase
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What is the underlying defect?
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Answer
• Its Trousseau’s sign, an indication of latent tetany
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What do you expect the plasma concentration of cholesterol in this patient ?
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Answer
• Higher than normal• It’s a case of Hypothyroidism.
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Which type of fatty acid is used for esterification of cholesterol by ACAT ?
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Answer
• Mono unsaturated fatty acid
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Name one precipitating factor for the disease
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Answer
• Alcohol• It is gouty arthritis
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Ribozyme ???
• Give one example of RNA acting as an enzyme.
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Answer
• Peptidyl Transferase in translation
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Use of AMP ?
• Give an example where AMP part of ATP is incorporated in to the substrate.
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Answer
• Conversion of FMN to FAD
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What is the significance of red line in this diagram?
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Answer
• It indicates shine Delgarno sequence• The small subunit/IF3 complex binds to the mRNA.
Specifically, it binds to the sequence AGGAGG, known as the Shine-Delgarno sequence, which is found in all prokaryotic mRNAs.( Its required for the initiation of protein synthesis)
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.
The central dogma states that for a given gene and gene product there is a linear relationship between the coding sequence in the DNA, the m- RNA sequence, and the protein sequence.
Is there any exception to the rule if so then give example and signify the purpose of this change ?
RNA editing ?
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Answer
• Editing of Apo – B m- RNA in the intestine• Apo B 48 is a component of chylomicrons
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IDENTIFY THE INSTRUMENT
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Answer
• HPLC- High Performance Liquid Chromatography for the separation or purification of Biomolecules.
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MEGX
What is the full form ?
What is the significance ?
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Answer
1. MEGX Test (Mono ethyl Glycine xylidine) is a liver function test based on detoxifying functions of liver.
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What will the effect on Renal Glutaminase activity in this patient ?
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Answer
• It is signifying Respiratory Acidosis. Kidneys will tend to conserve bicarbonates and excretion of H+ will increase, thus the glutaminase
activity will increase.
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Which complex in Electron Transport Chain does not have the Fe S center?
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Answer
Complex IV- (Cytochrome a-a3 oxidase complex)
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PROBLEM
• The hypothetical ‘stimulin’ gene contains two exons that encode a protein of 100 amino acids. They are separated by an intron of 100 bp beginning after the codon for amino acid 10. Stimulin messenger RNA has 5’ and 3’ untranslated regions of 70 and 30 nucleotides respectively. A complementary DNA made from mature stimulin RNA would have how many bp?
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Answer
• 400 bp
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Problem
• Which of the following involves Isoprenoids ?
• a) The chromophore of visual pigment• b) Carnitine• c) Thiamine• d) Ketone bodies
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Answer
a) The chromophore of visual pigment
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Problem
• What is the defect in Hereditary Non polyposis colon cancer (HNPCC)?
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Answer
• Faulty mismatch repair
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Problem
• Name one molybdenum requiring enzyme.
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Answer
• Xanthine oxidase
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Problem
• Chitin is a polymer of ?
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Answer
• The repeating unit in Chitin is N-Acetyl Glucosamine.
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What is this structure?
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Answer
• β meander- Super secondary protein structure
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What is the role of this vitamin in Glycogen metabolism ?
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Answer
• It acts as a prosthetic group for the Muscle Phosphorylase enzyme.
• So helps in muscle glycogenolysis.
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What is the Reichert- Meissl Number for Butter ?
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Answer
• Butter has high Reichert- Meissl Number and is the only fat with high number