Post on 30-Jun-2020
Background
Background
Background
Background
Museum! Museum!
Task
Design
step1：Define the city step2：Build a database for the city
Design
Design
How to build a bio-database
Design
Specific enzyme cutting sites
step4：Find every possible way
step5 : Find best way
step3：Choose the data
Design
1 2 3 4 5 6 7 8
Choosen City library
Choosen Path library
Design
How to find every possible routes?
Electrophoresis
DNA sequencing
Design
Obtain the specific results of pathway
Design
Bio-CPU
Design
Logical principles
BBa_K1341000
Design
Logical principles
BBa_K091101
The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50, 100, and
200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after
induction.
BBa_K1341000
Design
The advantage of LTT promoter
BBa_K1341001
No.5 Without
No.5
Fluorescenc
e output
1 0
Design
Step 1
No.5 N0.5&No.8
Fluorescence
output
1 0
Design
Step 2
No.5&N0.2&N0.8 N0.5&No.8
Fluorescence
output
0 0
Design
Step 3
Conditions are ±100 ng/mL aTc and ±1mM IPTG, All data is obtained 6 h after induction.
Fluorescence
output
No.5&N0.2&N0.8 N0.5&
No.8
N0.5&
No.2
Add IPTG 0(green) 1 0
Add aTc 1(red) 0 1
Design
Why add IPTG first?
Add IPTG No.5&N0.2&N0.8 N0.5&No.8
Fluorescence
output
0 1
Design
Step 4
Add
aTc
No.5&N0.2&N0.8
Fluorescence
output
1
The x and y axes form a grid of 36 pairs
of inducer concentrations. Behavior is
depicted 6 h after induction.
Design
Step 5
Result
Result of DNA sequencing
1
2 4
5
36 8
7
Result
Route:2-4-5-7-8 is the right result!
Human Practice&Help each other
College freshmen
“5089 college freshmen”
Human Practice
Human Practice
Military Training
Established community
Human Practice
Sina Blog APP
Human Practice
CCiC(Central China iGEMers' Consortium)
Human Practice
Help Each Other
Communicate with Central China iGEMers' Consortium:
BNU-China CAU_China HUST-China,
HZAU-China Jilin_China LZU-China,
NJU-QIBEBT NUDT_CHINA OUC-China,
Peking SCAU-China,SCUT WHU-China WHU-Pharm
Repair the debug of iGEM biobrick library:
WHU-China
WHU-Pharm
The questionnaire survey:
ETH Zurich HZAU-China LZU-China
BIT,SCUT BGU_Israel Michigan
Achievements
Submit 2 well document biobricks LTT GFP switch device:BBa_K1341000 Path way test system:BBa_K1341001Work as expected
Characterization of an existingBioBrickTTL promoter :BBa_K091101
Solving a bio-navigational probelm
Safety training
Attribute work done by the students .
Help registered iGEM team from another school.
Human practice
Thank you for listening!
P45: control plasmid copy number
Relative fluorescence: GFP/luciferase
WIKI
Project
Hierarchical Communication System
Future work