Operation Debug

NCTU_Formosa

Harmful Insect Elimination Force

Headquarters: National Chiao Tung University, Hsinchu, Taiwan

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Insect Damage

Current Solution: Pesticide

2

Motivation|

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Target |

Oriental Leafworm Moth

(Spodoptera litura)

Agricultural damage to

over 40 families of plants

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Tactical

Plan A : Biologically Synthesized Pheromone Traps

Plan |

5

Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths ( Russell Jurenka, and Ada Rafaeli, Oct 10 2011 )

Tactical

Plan B :

Plan |

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PBAN(Pheromone Biosynthesis Activating Neuropeptide)

Infiltration

Tactical Plan |

Gland

Receptor

PBAN

Pheromone

Biosynthesis Activated

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PBAN

Specialties

(1) Peptide

(2) Small

(3) Stable

(4) Specific

|

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Operation Detail |

Pheromone

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Biobrick

Biobrick |

RBS PBAN Ter

Pcons

PBANPBAN-SL

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Results – Making PBAN|

Oriental Leafworm Moth

(Spodoptera litura)

PCR

750 bp500 bp

SDS-PAGEControl

25 kDa

11 kDa

3.7 kDa

BBa_K1415105

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RBS PBAN-SL

Pcons

Results-Making PBAN|

BBa_K1415205

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PBAN-SL Pcons

BFPRBS RBS Ter

Control PBAN-SL

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Ca2+

Adenylate Cyclase

PBAN receptorCalmodulin

PBAN

G protein

Mechanism |

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CentrifugeAdd

Buffer

Pour out

LB

Vortex

MashDilute

Autoclave

Obtain Bio-safe PBAN solution

E.coli contain PBAN

Sonicator

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PBAN

Cotton

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Control

( Without PBAN )With PBAN

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• |

Parts of Device |

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Operational Process|

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Operational Process |

22

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Modeling|

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Device for light preference

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Result - PCR |

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SL

BM MB AI

LD HAH

AS SI AA

750 bp

MB AI LD SL HAH AS SI AA

500 bp

250 bp

BM

Result - SDS PAGE |

27

Control BM AA LD AS

25 kDa

11 kDa

AI MB HAH SIControl

2-4 kDa

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Control

SLBM

MBAI LD

HAHAS

SI

AA

PBAN-SL Pcons

BFPRBS RBS Ter

Human Practice

Cooperation |

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Industry Collaboration

Team ExchangeiGEM Promoting

Organic Farm |

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iGEM Conference |

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Bio-camp|

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Green Market |

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Achievements|

New basic and composite BioBrick parts

We converted 9 PBANs into standard parts and constructed 27 new BioBricks.

Successfully resolving insect damage

We developed a brand new control system and a device for its application.

Real application in industry

Organic farms are currently using our new system and loving it.

Team Meetups

We held an international conference with 22 iGEM teams from China and Taiwan.

Help any registered iGEM team from another school

We assisted TCU Taiwan iGEM Team with their modeling.

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Acknowledgement

Instructors:

Prof. Yan-Hwa Wu

Prof. Yuh-Jyh Jong

Prof. Hsien-Da Huang

Prof. Shin-Ying Ho

Prof. Hsiao-Ching Lee

Prof. Wen-Liang Chen

Mission

Accomplished