Post on 15-Jan-2016
advancing the state of the art in microscopy
Imaging Protein Crystals and Managing the
Associated Data
What Type of Microscope to Use?
• What Type of Microscope to Use?
– Sample• Liquid Drop w/ Solid and/or Semi Solid
• What Type of Microscope to Use?
– Sample• Liquid Drop w/ Solid and/or Semi Solid
– Sample Location• MicroBatch, Sitting Drop or Hanging Drop
Inverted Configuration
Light Source
Sitting DropOr
Microbatch Drop
Microscope
Upright Configuration
Microscope
Hanging Drop
Light Source
Upright Inverted
Sitting Drop Imaging Comparison
• What Type of Microscope to Use?
– Sample• Liquid Drop w/ Solid and/or Semi Solid
– Sample Location• MicroBatch, Sitting Drop or Hanging Drop
– Illumination• Koehler
Properties of a Drop
- Lens Effect
Properties of a Drop
- Lens Effect
- Side Wall/Refraction Ring
Less than 90º
Contact Angle of a Drop
Contact Angle of a Drop
Hemispherical
Contact Angle of a Drop
Greater than 90º
High Contrast = More Detail
Crystals at the Drop Edge - use medium contrast
Very dark side-wall effect - use low contrast
Focusing on condensation
Focusing on condensation Focusing on Crystals
Focusing on condensation Focusing on Crystals
Lo
w C
on
tras
tH
igh
Co
ntr
ast
L.I.M.S.
Laboratory Information Management System
Data Management
Barcodes
Crystallization Data
Experiment Details
Crystallization DataExperiment Details
Image Management
Scoring Images
Experiment Details
Image Management
Scoring Images
Reviewing Results and Trends
Crystallization Data
Experiment Details
Image Management
Scoring Images
Reviewing Results and Trends
Remote Viewing
Crystallization Data
Crystallization DataExperiment Details
Image Management
X-Ray Data
Experiment Details
Image Management
X-Ray Data
Printing Results
Crystallization Data