Post on 31-Dec-2015
A novel method for the in vivo isolation of circulating tumor cells from peripheral
blood of cancer patients using afunctionalized and structured medical wire
Presenter:Wei-Siang Ciou Adviser: Dr.Yi-Chun Du
Date: 2014/11/19
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Saucedo-Zeni, N., Mewes, S., Niestroj, R., Gasiorowski, L., Murawa, D., Nowaczyk, P., ... & Lücke, K. (2012). A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of
cancer patients using a functionalized and structured medical wire. International journal of oncology,41(4), 1241-1250.
Outline
Abstract
Introduction
Materials and methods
Results
DiscussionIBS_LAB
Abstract (1/2)
Circulating tumor cells
To overcome the limitations of small blood
sample volumes of the ex vivo CTC isolation
techniques
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Suitability 、 specificity and sensitivity
Tested in 24 breast cancer or non-small cell lung
cancer patients and in 29 healthy volunteers
Found to be safe with no noteworthy side effects
Abstract (2/2)
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Introduction (1/4)
Personalized cancer treatment
Improve health and quality of life of cancer
patients
Different tumor cells are distinct on the molecular
level
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Introduction (2/4)
Circulating tumor cells(CTCs)CTCs detection flow chart
CellSave採血管:用於收集並儲存週邊靜脈血液
取7.5ml血及6.5ml緩衝液放入離心管離心
Celltracks Autoprep:自動對CTCs進行捕獲和染色,可同時
對8個樣本進行處理
MagNest:將捕獲、經染色的細胞置入MagNest裝置中,在其磁場作用下使靶細胞移到分析表面
Celltracks AnalyzerII:用於對經過免疫磁顆粒選定與螢光染料標記的CTCs進行記數
http://www.gendiscovery.com.tw/pdf/檢測循環腫瘤細胞研究進展 .pdfIBS_LAB
Introduction (3/4)
Alternative medical device
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Introduction (4/4)
Photoacoustic flow cytofluorometry Schematic drawing of an in vitro flow
system for repeated interaction of CTC with the FSMW, and cancer cells captured by the FSMW.
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Materials and methods (1/7)
Functionalized structured medical wire
0.5 mm in diameter and 160 mm in length
The first 20 mm are plated with a 2 µm thick gold
layer deposited on the device by galvanization
A hydrogel layer composed of a linear
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Materials and methods (2/7)
In vitro experiments
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Materials and methods (3/7)
Principle of peristaltic pump
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Materials and methods (4/7)
Principle of peristaltic pump
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Materials and methods (5/7)
Cytotoxicity tests
Acute systemic toxicity
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Materials and methods (6/7)
Hemocompatibility test
Assessment of the patient's risk to develop
thrombosis
Coagulation and Hemolysis after in vivo
exposure to the FSMW
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Materials and methods (7/7)
In vivo application of the FSMW
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Result (1/5)
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Result (2/5)
Cancer cells captured by the FSMW
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Result (3/5)
Biocompatibility of the FSMW deviceA. Teflon wire eluate : No
apparent changes in cell morphology.
B. Copper wire eluate : Severe changes in cell morphology.
C. FSMW eluate prepared from a singl FSMW :No apparent change in cell morphology.
D. FSMW eluate prepared from three FSMW:
No apparent change in cell morphology.
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Result (4/5)
In vivo application of the FSMW in
healthy volunteers and in breast cancer
and NSCLC patients
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Result (5/5)
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Discussion (1/3)
The main cause of death from cancer
Most histopathologic studies investigated
primary tumor tissues only
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Discussion (2/3)
CTC in the peripheral blood of cancer
patients exist in extreme rarity
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Discussion (3/3)
Prospective clinical cancer trials.
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Thanks for Your Attention
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