A novel method for the in vivo isolation of circulating tumor cells from peripheral

blood of cancer patients using afunctionalized and structured medical wire

Presenter：Wei-Siang Ciou Adviser： Dr.Yi-Chun Du

Date： 2014/11/19

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Saucedo-Zeni, N., Mewes, S., Niestroj, R., Gasiorowski, L., Murawa, D., Nowaczyk, P., ... & Lücke, K. (2012). A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of

cancer patients using a functionalized and structured medical wire. International journal of oncology,41(4), 1241-1250.

Outline

Abstract

Introduction

Materials and methods

Results

DiscussionIBS_LAB

Abstract (1/2)

Circulating tumor cells

To overcome the limitations of small blood

sample volumes of the ex vivo CTC isolation

techniques

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Suitability 、 specificity and sensitivity

Tested in 24 breast cancer or non-small cell lung

cancer patients and in 29 healthy volunteers

Found to be safe with no noteworthy side effects

Abstract (2/2)

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Introduction (1/4)

Personalized cancer treatment

Improve health and quality of life of cancer

patients

Different tumor cells are distinct on the molecular

level

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Introduction (2/4)

Circulating tumor cells(CTCs)CTCs detection flow chart

CellSave採血管:用於收集並儲存週邊靜脈血液

取7.5ml血及6.5ml緩衝液放入離心管離心

Celltracks Autoprep:自動對CTCs進行捕獲和染色，可同時

對8個樣本進行處理

MagNest:將捕獲、經染色的細胞置入MagNest裝置中，在其磁場作用下使靶細胞移到分析表面

Celltracks AnalyzerII:用於對經過免疫磁顆粒選定與螢光染料標記的CTCs進行記數

http://www.gendiscovery.com.tw/pdf/檢測循環腫瘤細胞研究進展 .pdfIBS_LAB

Introduction (3/4)

Alternative medical device

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Introduction (4/4)

Photoacoustic flow cytofluorometry Schematic drawing of an in vitro flow

system for repeated interaction of CTC with the FSMW, and cancer cells captured by the FSMW.

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Materials and methods (1/7)

Functionalized structured medical wire

0.5 mm in diameter and 160 mm in length

The first 20 mm are plated with a 2 µm thick gold

layer deposited on the device by galvanization

A hydrogel layer composed of a linear

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Materials and methods (2/7)

In vitro experiments

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Materials and methods (3/7)

Principle of peristaltic pump

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Materials and methods (4/7)

Principle of peristaltic pump

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Materials and methods (5/7)

Cytotoxicity tests

Acute systemic toxicity

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Materials and methods (6/7)

Hemocompatibility test

Assessment of the patient's risk to develop

thrombosis

Coagulation and Hemolysis after in vivo

exposure to the FSMW

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Materials and methods (7/7)

In vivo application of the FSMW

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Result (1/5)

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Result (2/5)

Cancer cells captured by the FSMW

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Result (3/5)

Biocompatibility of the FSMW deviceA. Teflon wire eluate ： No

apparent changes in cell morphology.

B. Copper wire eluate ： Severe changes in cell morphology.

C. FSMW eluate prepared from a singl FSMW ：No apparent change in cell morphology.

D. FSMW eluate prepared from three FSMW:

No apparent change in cell morphology.

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Result (4/5)

In vivo application of the FSMW in

healthy volunteers and in breast cancer

and NSCLC patients

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Result (5/5)

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Discussion (1/3)

The main cause of death from cancer

Most histopathologic studies investigated

primary tumor tissues only

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Discussion (2/3)

CTC in the peripheral blood of cancer

patients exist in extreme rarity

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Discussion (3/3)

Prospective clinical cancer trials.

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Thanks for Your Attention

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