13ListedCarcinogensChemicalClassStandardOperatingProcedure

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13ListedCarcinogens

Note:DuetotheuniqueCal/OSHAregulationsinTitle8§5209 thatapplytothese13listedcarcinogens

(signage,PPE,handling,disposal),anynewusermustbetrainedbyanEH&Srepresentativeoraqualifieddesigneebeforestartinganywork.

ThisSOPisnotasubstituteforhands-ontraining.

PrintacopyandinsertintoyourlaboratorySOPbinder.

Department: Chemistry

DateSOPwaswritten: Thursday,March23,2017

DateSOPwasapprovedbyPI/labsupervisor: Friday,March24,2017

PrincipalInvestigator:Name:F.Fischer

Signature:______________________________

InternalLabSafetyCoordinatororLabManager:

Name:StephenvonKugelgen

LabPhone:510.301.1058

OfficePhone:510.642.8252

EmergencyContact:Name:StephenvonKugelgen

PhoneNumber:510.301.1058

Location(s)coveredbythisSOP: TanHall674,675,676,679,680,683,684

1. Purpose

ThisSOPcoverstheprecautionsandsafehandlingproceduresfortheuseof13ListedCarcinogens.

Foralistof13ListedCarcinogenscoveredbythisSOPandtheiruse(s),seethe“ListofChemicals”.ProceduresdescribedinSection13applytoallmaterialscoveredinthisSOP.

Ifyouhavequestionsconcerningtheapplicabilityofanyrecommendationorrequirementlistedinthisprocedure,contactthePrincipalInvestigator/LaboratorySupervisororthecampusChemicalHygieneOfficeratucbcho@berkeley.edu.

2. DefinitionofChemicalGroup

Theterm“listedcarcinogen”referstoaspecificlistof13chemicalsregulatedbyCal/OSHA.Thesechemicalshavespecificuseandhandlingrequirements.Allhandlingoruseofthe13“Listed”

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carcinogensbelowrequiresevaluationbyEH&SandreportingtoCal/OSHA,evenifworkiscontainedwithinalaboratoryfumehood.

1. 2-Acetylaminofluorene

2. 4-Aminodiphenyl

3. Benzidine(anditssalts)

4. 3,3'-Dichlorobenzidine(anditssalts)

5. 4-Dimethylaminoazobenzene

6. alpha-Naphthylamine

7. beta-Naphthylamine

8. 4-Nitrobiphenyl

9. N-Nitrosodimethylamine

10. beta-Propiolactone11. bis-Chloromethylether

12. Methylchloromethylether

13. Ethyleneimine

3. PotentialHazards/Toxicity

Thetermcarcinogendenotesasubstanceoramixturewhichinducescancerorincreasesitsincidence.The13ListedCarcinogensarechemicalsthatcausecancerortumordevelopment,typicallyafterrepeatedorchronicexposure.Theireffectsmayonlybecomeevidentafteralonglatencyperiodandmaycausenoimmediateharmfuleffects.

TheGloballyHarmonizedSystemofClassificationandLabelingofChemicals(GHS)designatescarcinogenicsubstancesbyoneormoreofthefollowingHcodes:

H350Maycausecancer

H351Suspectedofcausingcancer

Carcinogensmayalsohaveotherhazardouspropertiesinadditioncarcinogenicity.Safeuserequiresassessingallpotentialhazards.ItisthePrincipal Investigator’s responsibility toensureactivity-specific laboratoryproceduresand/orprocessesaretakenintoaccountwhenusingthisChemicalClassSOP.Please,reviewtheSDSofanychemicalbeforeuse(seeSection12–SDSLocation).

4. PriortoBeginningWork

• Allhandlingoruseofthe13ListedCarcinogensrequiresevaluationbyEH&SandreportingtoCal/OSHA.ContactEH&Sat642-3073orehs@berkeley.edutoscheduleanevaluation.

• Researchersmusthavehands-ontrainingbyaknowledgeabledesignee.Worktechniquemustbeobservedandindependenthandlingofcarcinogenslimitedtoresearchersthathavedemonstratedcompetency.

• Researchersmustbeawareofthesafehandlingofthechemical,itsphysicalproperties(includingsolubility),andhealtheffectsseeninexperimentalstudiesandotherapplicablesources.

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• Addmaterialtothechemicalinventory,andmaintainanaccurateinventoryofthematerialatalltimes.

5. EngineeringControls

Usetheengineeringcontrolslistedbelowunlessotherlab-specificinformationisincludedinSection13-Protocol/Procedure.

• Workwiththe13ListedCarcinogensmustbeconductedinafumehoodunlessothercontrolsaredesignatedinthelab-specificProtocol/Proceduresection.Sashheightmustbekeptlowtoavoidescapingfumesandprovideaphysicalbarrier.Theremustbeadesignatedareaforworkingwiththese13listedcarcinogens.Thisareamustbeappropriatelylabeled.

• Laboratoriesandroomswhereany13listedcarcinogensareusedmusthavegeneralroomventilationthatisnegativepressurewithrespecttothecorridorsandexternalenvironment.Thelaboratory/roomdoormustbekeptclosedatalltimes.

6. PersonalProtectiveEquipment

Ataminimum,thefollowingPPEmustbewornatalltimes.

EyeandFaceProtection

A. ANSIZ87.1-compliantsafetyglasseswithsideshields,orchemicalsplashgoggles.

• Ordinary prescription glasses will NOT provide adequate protection unless they alsomeetANSIstandardandhavecompliantsideshields.

B. Ifthepotentialforexplosion/splashingexists,andadequatecoverageisnotprovidedbythehoodsash,afaceshieldmustbeworn.

SkinandBodyProtection

A. Glovesarerequiredwhenhandlinghazardouschemicals.

• RefertospecificchemicalSDSforinformationongloveselection.

• Foradditionalinformationongloveselection,goto:

http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-and-usage.html

B. Labcoatsarerequiredwhenhandlinghazardouschemicalsinthelab.Selectthetypeoflabcoataccordingtothehazardsatthespecificworkplace.

C. Longpants,closed-toe/closed-heelshoes,coveredlegs,andankles.

RespiratoryProtection

RespiratoryprotectionisnormallynotrequiredforUCBerkeleylaboratoryactivities.Anylabpersonnelconsideringtheuseofarespirator(e.g.N-95respirator,dustmask)mustcontactEH&Sforaworkplaceassessment.

7. FirstAidProceduresandMedicalEmergencies

Intheeventofaninjury,notifyyoursupervisorimmediatelyandEH&Swithin8hours.

GototheOccupationalHealthFacility(TangHealthCenter,oncampus); ifafterhours,gotothenearestemergencyroom(AltaBates,2450AshbyAveinBerkeley);or

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Call911(fromacellphone:510-642-3333)if:

• itisalifethreateningemergency;or

• younotareconfidentinyourabilitytofullyassesstheconditionsoftheenvironmentand/orthe condition of the contaminated/injured person, or you cannot be assured of your ownsafety;or

• thecontaminated/injuredpersonisnotbreathingorisunconscious.

Please remember to provide a copy of the appropriate manufacturer SDS (if available) to theemergency responders or physician. At a minimum, be ready to provide the identity/name of anyhazardousmaterialsinvolved.IncaseofskincontactIfskincontactoccurs,and/orskinorclothingareonfire,immediatelydrenchinthesafetyshowerwithcopiousamountsofwaterfornolessthan15minutestoremoveanyremainingcontaminants.Ifpossibletodosowithoutfurtherinjury,removeanyremainingjewelryorclothing.

IncaseofeyecontactRinsethoroughlywithplentyofwaterusinganeyewashstationforatleast15minutes,occasionallyliftingtheupperandlowereyelids.Removecontactlensesifpossible.IfswallowedDoNOTinducevomitingunlessdirectedotherwisebytheSDS.Nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater.IfinhaledMoveintofreshair.Needlestick/punctureexposureWashtheaffectedareawithantisepticsoapandwarmwaterfor15minutes.

8. SpecialHandling,StorageandDisposalRequirements

Lab-specificinformationonhandlingandstoragemaybeincludedintheProtocol/Proceduresection.Ifhandlingoneofthe13ListedCarcinogens,thefollowingcontrolsapplyper8CCR5209(c)(6).

RegulatedWorkAreas

Aregulatedareacanbetheentirelaboratoryoralaboratoryhood,butmustindicateaworkareaoflimitedaccesswherespecialprocedures,knowledge,andworkskillsarerequired.Regulatedareasmustbeclearlymarkedwithsignsthatidentifythechemicalhazardandincludeanappropriatewarning:

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PrecautionsforSafeHandling

• Eliminateorsubstituteforalesshazardousmaterialwhenpossible.• Designyourexperimenttousetheleastamountofmaterialpossibletoachievethedesired

result.• DonotexceedthescaleofproceduresspecifiedinProtocol/Proceduresectionwithoutapproval

ofthePI.• Chemicalsmustbeclearlylabeledwiththechemical’snameandhazards.• Knowthelocationofthenearesteyewash,safetyshowerandfireextinguisherbeforebeginning

work.• Laboratoryworksurfacesonwhichacarcinogenishandledmustbeprotectedfrom

contamination.Lineworksurfacewithabsorbent,removablematerial.Providesecondarycontainmentforchemicals.

• Aswithanylaboratorychemical,donotmouthpipettesolutions.• Usecontainmentdevices(suchaslabfumehoodsorgloveboxes)whenopenhandlingis

required.Ensureyourlaboratoryfumehoodisfunctioningproperly.• Forliquidtransfer,thereagentcanbedispensedusingasyringe.• Useplasticsyringesonlyonce;therubbergasketofaplasticsyringemayswellupleadingtoa

jammedsyringe.• Useventilatedcontainmenttoweighoutsolidchemicals.Alternatively,thetaremethodcanbe

usedtopreventinhalationofthechemical.Whileworkinginalaboratoryfumehood,thechemicalisaddedtoapre-weighedcontainer.Thecontaineristhensealedandcanbere-weighedoutsideofthefumehood.Ifchemicalneedstobeaddedorremoved,thismanipulationiscarriedoutinthefumehood.Inthismanner,allopenchemicalhandlingisconductedinthelaboratoryfumehood.

• Fumehoodmustbecheckedeverysix(6)months(callEH&Sat642-3073ifyourfumehoodindicatedmorethansix(6)monthshavepassedsincethelastcheck).

• Whenhandlingoneofthe13ListedCarcinogens,dailychangeofpersonalprotectiveequipmentisrequired:researchersarerequiredtoremoveandleaveprotectiveclothingandequipmentatthepointofexitfromthedesignatedworkareaand,atthelastexitoftheday,toplaceusedclothingandequipmentinimperviouscontainersfordecontaminationordisposal.Labcoatstobelaunderedmustbeplacedinaclearlylabelled,sealedplasticbag(seeSection11.HazardousWasteDisposalandLabCoatlaundering).Contaminatedequipment(gloves,wipes,disposablesyringes,etc)mustbethoroughlyrinsed,placedinsealedplasticbaganddisposedofintheregularsolidwastebin.

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• Usehighefficiencyparticulateair(HEPA)filters,carbonfilters,orscrubbersystemswithcontainmentdevicestoprotecteffluentandvacuumlines,pumps,andtheenvironment.

• Researchersarerequiredtowashhands,forearms,faceandneckuponeachexitfromthedesignatedcarcinogenworkarea.Theuseofwetwipes(likebabywipes)isacceptableforfaceandneckwash.

ConditionsforSafeStorage

• Storecarcinogensinclosed,labeled,andchemicallycompatiblecontainersandawayfromheat,flame,andotherreactivechemicalsthatmaydispersethecarcinogenifmixedduringanaccidentalrelease.

• Alwaysplacelarge-volumecontainersonalow,protectedshelforinanotherlocationwheretheywillnotbeaccidentallyspilledorknockedover.

• 13ListedCarcinogensmustbestoredinadesignatedarea.Thelabel“carcinogen”mustbeclearlyindicatedonthecontainerorshelf.

• Storenonflammablecarcinogenswithinsecondarycontainment.• Chemicalsthatrequirerefrigerationmustbestoredappropriately.• Storeflammablecarcinogenswithinflammablestoragecabinetandsecondarycontainer.

Disposal

● Wastematerialsgeneratedmustbetreatedasahazardouswaste.● TheemptycontainermustberinsedthreetimeswithaCOMPATIBLEsolvent;leaveitopen

inthebackofthehoodovernight.Solventrinsesandwaterrinsemustbedisposedofashazardouswaste.

● Asanalternative,unrinsedemptycontainerscanbedisposedofthroughEH&Sashazardouswaste.Theunrinsedemptycontainersmustbecapped.

● Donotmixwithincompatiblewastestreams.● Decontaminationoftheemptycontainerinordertouseitforotherpurposesisnot

permitted.

9. ChemicalSpill

Spill–Assesstheextentofdanger;ifnecessaryrequesthelpbycalling911(fromacellphone:510-642-3333)foremergencyassistanceor510-642-3073fornon-lifethreateningsituations.Ifyoucannotassesstheconditionsoftheenvironmentwellenoughtobesureofyourownsafety,donotenterthearea.Ifpossiblehelpcontaminatedorinjuredpersons.Evacuatethespillarea.Avoidbreathingvaporsfromspill.Ifpossible,confinethespilltoasmallareausingaspillkitorabsorbentmaterial.Keepothersfromenteringcontaminatedarea(e.g.,usecautiontape,barriers,etc.).

• MinorSpill–Intheeventofaminorspill,ifthereisnopotentialforhazardouschemicalexposure,reportthespillto510-642-3073andifyouaretrained,proceedtocleanit.Useappropriatepersonalprotectiveequipmentandclean-upmaterialforchemicalspilled.Doublebagspillwasteinclearplasticbags,labelandrequestpick-up.

• MajorSpill–Anyhazardouschemicalspillthatinvolveschemicalexposure,anychemicalspillthatduetosizeand/orhazardrequirescapabilitiesbeyondyourtraining,oranychemicalspillthatgivestheperception(becauseofodor,forexample)thattherehasbeenahazardousrelease.Call911or510-642-3073forassistance.

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10. CleaningandDecontamination

Lab-specificinformationondecontaminationmaybeincludedintheProtocol/Proceduresection.

• WearingproperPPE,laboratoryworksurfacesmustbecleanedattheconclusionofeachprocedureandattheendofeachworkday.

• Uponleavingthedesignatedworkarea,removeanypersonalprotectiveequipmentwornandwashhands,forearms,faceandneck.

• Attheendofeachproject,thoroughlydecontaminatethedesignatedareabeforeresumingnormallaboratoryworkintheareaandremovingcarcinogenwarningsigns.

11. HazardousWasteDisposalandLabCoatlaundering

CarcinogenWasteDisposal

• Disposableglovesslightlycontaminatedwithchemicalsmustbethoroughlyrinsedbeforebeingdiscardedintheregulartrash.

• Forheavilycontaminatedequipment(gloves,wipes,disposablesyringes,etc),placeinachemically-compatibleimperviouscontainer(i.e.sealableplasticbag,containerwithtwistonlid)andlabelclearly.Decontaminateoutsideofcontainerpriortoremovalfromtheworkarea;storewithinafumehooduntilpickupbyEH&S.

• Whenfeasible,carcinogensmustbeinactivatedpriortodisposal.• Disposeofregularlygeneratedchemicalwastewithin6months.• ContactEH&Sat642-3073ifyouneedassistance.

LabelWaste

• Label all waste containers. See the EH&S Fact Sheet, “Hazardous Waste Management” forgeneralinstructionsonproceduresfordisposingofhazardouswaste.

LabCoatLaundering

• Whenhandlingoneofthe13ListedCarcinogens,launderinglabcoatatthelastexitofthedayisrequired.Belowisanexampleoflabelthatcanbeused.

12. SafetyDataSheet(SDS)LocationSDScanbeaccessedonlineathttp://ucsds.com

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13. Protocol/Procedure–ListedCarcinogens

Procedure/Use Scale EngineeringControls/Equipment

PPE(eye,face,gloves,clothing) ProcedureStepsandPrecautions

1.UsingListedCarcinogenicchemicalsasreagents.

Upto1gofsolidor20mLofliquidorsolutionassuppliedinthereagentbottle.RemembertoobtainPIapprovalifhigherscaleisnecessary.Documentthisapprovalinyournotebook

Allreactionsusingthesematerialsmustbeperformedinaproperlyoperatingfumehoodwiththesashaslowaspossibleorinaninertatmosphereglovebox.

Eyeprotection:WearANSIapprovedtight-fittingsafetygogglesorsafetyglasseswithsideshields.FaceProtection:Theneedforfaceprotectionisbasedonthesolventsusedorifthereactionwillbeexternallyheatedorifneeded.HandProtection:Confirmcompatibilityofglovematerialwithchemicalbeingused.Generalguidance(unlessotherwisespecifiedinthespecificSDS):Nitrileglovesmustbeusedtopreventincidentalcontact.Forspillhandlingorforpotentialcontactwithlargerquantities,usedoublenitrileorheaviergaugenitrileorneoprenegloves.Glovesmustbeinspectedpriortouse.Washanddryhandsafteruse.Clothing:Wearlabcoat;fulllengthpantsorequivalent;andclose-toed,close-heeledshoes.

Putupanappropriatehazardsignonyourfumehoodtoalertotherlabusers.Avoidtheformationofdustswithsolids.Carefullyweighmaterialsasnormalinasealedcontainer.Transfersolidsintoatared,containerinafumehood,usingsecondarycontainmenttomovethesealedcontainertoandfromthefumehoodduringweighing.Inaproperlyfunctioningfumehood,addreagenttothereactionvessel.Ifthereagentisaliquid,dispenseandtransfertothereactionvesselvolumetricallyusingeitherasyringeorpipettor.CleanupworkareaanddisposeofcontaminatedmaterialsasoutlinedinSections10&11.

Notes AnydeviationfromthisSOPrequiresapprovalfromPI.

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14. DocumentationofTraining(signatureofallusersisrequired)

• Priortoconductinganyworkwiththe13ListedCarcinogens,designatedpersonnelmustprovidetrainingtohis/herlaboratorypersonnelspecifictothehazardsinvolvedinworkingwiththissubstance,workareadecontamination,andemergencyprocedures.

• ThePrincipalInvestigatormustprovidehis/herlaboratorypersonnelwithacopyofthisSOPandacopyoftherelevantSDSsprovidedbythemanufacturer.

IhavereadandunderstandthecontentofthisSOP:

Name Signature Identifier Date

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ListofChemicals

Chemical(s) Chemical(s) Chemical(s)1-Naphthylamine 2-Naphthylamine 3,3'-Dichlorobenzidine 4-Aminobiphenyl 4-Nitrobiphenyl Benzidine beta-Propiolactone bis-Chloromethyl Ether Butter Yellow Chloromethyl methyl ether Ethyleneimine N-(2-Fluorenyl)acetamide N-Nitrosodimethylamine