Protein Purification Lab C2 Pages 115 to 168 Lab C.2 Four Periods Protocol Page 135-160 Benchtop Protocols begin on page 398 Be sure to read theory starting.
WATER By KHAIRUL FARIHAN KASIM. Chapter 3: Outline Molecular Nature of Water Noncovalent Bonding –Ionic interactions –Hydrogen Bonds –van der Waals.
1.Solvation Models and 2. Combined QM / MM Methods See review article on Solvation by Cramer and Truhlar: Chem. Rev. 99, 2161-2200 (1999)
Protein purification always begin with intact tissue Disrupt Blender, homogenizer Remove debris Centrifugation Precipitate/concentrate Ammonium.
Solvation Models and 2. Combined QM / MM Methods
