Genome Sizes are Large human = 3 x 10 9 bp E. coli = 4 x 10 6 bp If 1 bp = 1 mm, then: human genome = 3000 km (1800 miles) E. coli genome = 4 km (2.5 miles)
Transgenic plants new
45059502-Dna-Isolation.ppt
BIOTECHNOLOGY What can we do with DNA?. Biotechnology Manipulation of biological organisms or their components for research and industrial purpose Usually.
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Cell Structure: What we need to Extract: DNA EXTRACTION.
DNA Methodologies Sterilization –Clean the workstation with alcohol and bleach. –Autoclaving and ultraviolet light (UV radiation). Consumables and reagents.
Extraction of Human DNA
Direct DNA transfer Introduce DNA into cells; assay expression immediately or select for permanently transformed cells. Techniques: 1.Chemical induction.
Extraction of Human DNA. Experiment Goals Isolation of genomic DNA from human blood Analysis of isolated DNA using –Agarose gel electrophoresis –Spectrophotometry.
