MOLECULAR BIOLOGY – Molecular biology techniques MOLECULAR BIOLOGY TECHNIQUES I. DNA isolation and fragmentation Recombinant DNA Gel electrophoresis Hybridization.
Introduction to Epigenetics/Epigenomics Xiangqin Cui Department of Biostatistics University of Alabama at Birmingham Workshop on Statistical Genetics and.
DNA, Chromosomes and the Nucleus. DNA Structure What are some of the important structural features of the DNA double helix?
Pglo experiment What is ampicillin? A cell wall inhibiting antibiotic What happens to normal bacteria that are grown on agar plates with ampicillin in.
Biological background: Molecular Laboratory Techniques Class web site: Statistics for Microarrays.
Allele specific PCR or ARMS test Amplification Refractory Mutation System Used to detect point-mutations and small deletions, differentiates between DNA-sequences.
Southern Blot Hybridization Outline of Lecture n Basic steps n Purpose n Blotting methods u Mechanics n Membrane choices u Blotting solutions n Blocking.
Isolation of DNA. DNA Deoxyribonucleic acid Genetic material of all living things Makes up chromosomes of living things –Contained within a nucleus in.
Spring 2007 Bioinformatiatics Ch. 6 - Genomics. Completed genomes Spring 2009 Bioinformatiatics .
Review of important points from the NCBI lectures. –Example slides Review the two types of microarray platforms. –Spotted arrays –Affymetrix Specific examples.