Selection of XID target site Ani-wt: T G A G G A G G T T T C T C T G T A A m-XID: A G T G C C T G T T T C T C T T G A C -10 -9 -8 –7 –6 –5 -4 –3 –2 –1.
Yupeng. Yeast surface Aga1p s s s s Aga2p I-AniI Myc 3’-ACTCCTCCAAAGAGACATT 5’-TGAGGAGGTTTCTCTGTAA Biotin Ani-wt: T G A G G A G G T T T C T C T G T A.
Thermal stability of I-AniI variants
Selection of XID(Btk) site specific I-AniI variant for gene repair in hematopoietic stem cells
