Enzyme Engineering
1 Site Directed Mutagenesis and Protein Engineering BC35C Biotechnology I (Lecture notes 2004) Prepared and presented by Dr. Marcia E. Roye Office: Biotechnology.
Enzyme catalysis Basic concepts in chemical catalysis The Michaelis-Menten model of enzyme kinetics Structure-function relationship: the serine protease.
Protein engineering
Bernard R. Glick Molecular Biotechnology Text: Molecular Biotechnology B.R. Glick, J.J. Pasternak and C.L. Patten ASM Press, Fourth Edition.
1 Directed Mutagenesis and Protein Engineering. 2 Mutagenesis Mutagenesis -> change in DNA sequence -> Point mutations or large modifications Point mutations.
Chapter 8 Mutagenesis of cloned DNA. To creating numerous mutations in a small DNA sequence, mutagenesis with degenerate oligonucleotides a palindromic.
