Expression vectors
Dna cloning
F tools of genetic engineering
Principles of Biochemistry Fourth Edition Chapter 23 Recombinant DNA Technology Copyright © 2006 Pearson Prentice Hall, Inc. Horton Moran Scrimgeour Perry.
Doug Brutlag 2011 Sequencing the Human Genome Doug Brutlag Professor Emeritus of Biochemistry.
Chp 1 Basic principles of Gene Cloning. What is cloning? Molecular cloning Cell cloning Organism cloning What is gene cloning?
Research interests of Dr Konstantinos Papadimitriou Research associate Laboratory of Dairy Research Department of Food Science and Human Nutrition Agricultural.
GENETIC ENGINEERING. MANIPULATING GENES… Can we make our food taste better? Can we make humans live longer? Can we make X-men like mutants?!? Let’s start.
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings p.174.
Reproductive cloning Biotechnology/Cloning. Reproductive cloning.
Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms.
DNA Synthesis Eukaryotes DNA unwinds at a replication origin Two replication forks form Bidirectional synthesis of DNA Much more DNA; complexed with proteins.