Clean genome ecoli
Genomic Circuits. Are Genes Hardwired? No, gene expression can be modified Cis-regulatory elements are modular; offering sites for protein binding in.
CLEAN GENOME E. COLI – MULTIPLE DELETION STRAINS Gulpreet Kaur Microbial Biotechnology, Fall 2011.
CLEAN GENOME E. coli – multiple deletion strains
RT-PCR Obtain the internal region of cDNA
RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 65 0 C for 3-15.