UNIVERSITY OF THE PUNJABSeventh Semester - 2019

Examination: B.S. 4 Years Program

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', .PAPER: General MicrobiologyCourse Code: ZOOL-415 Part-I (Compulsory)

Q.l. Encircle the right answer, cutting and overwriting is not allowed. (lxlO=lO)

- .. - -~ - ---- - ~-. -------- ---:-- __ ~_ Bacteria require low levelof ~~-yg~nfor- groWthand cannot tolerate atmospheric oxygen level .A) AerobicB) AnaerobicC) FacultativeD) MicroaerophilicE) None ofthese

ii .: Numerical Taxonomy depends on~) % similarity index of strains to be comparedB) Intuitive method of classifying bacteriaC) Genetic relatednessD) Predictability of a classification schemeE) None of theseThe descendants of a single isolation in pure culture are called asA) SpeciesB) Type speciesC) strainD) None of theseEndospores are heat resistant because it contains:A) Calcium-dipicolinic acidB) Poly-f3-hydroxybutyrateC) PolyisoprenoidD) HeteropolysaccharidesE) None of theseWhich microorganism cannot be grown on artificial media?A) Protozoa 'B) YeastC) AlgaeD) VirusE) None of theseViable bacterial cells can be counted by:A) Turbidimetric methodB) Direct microscopic countC) Membrane filter countD) Electronic enumeration of cellsE) None of these

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VII. Application of ionIzing . d' t'- .-. - " . . . ..__ _ ~__ ~~~_.~a.IOns to stenlIze bIOlogical materials is

A) Incineration . -- --~. -= ~= ~- . ~~- --

B) Cold sterilizationC) Fractional sterilizationD) DesiccationE) None of thesePellicle is present in ------A) BacteriophageB) ProtozoaC) AlgaeD) FungiE) None of these-- was the first person to recognize significance ofbacterial and other microbes in disease:A) Robert HookeB) Roger BaconC) LeeuwenhoekD) Anton Von PlencizE) None of theseDuring this phase, the microbial cells divide steadily at a constantrate and log of the number of cells plotted against time results in astraight lineA) Lag phaseB) Exponential phaseC) Stationary PhaseD) None ofthese

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UNIVERSITY OF THE PUNJABSeventh Semester - 2019

Examination: B.S. 4 Years Program

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PAPER: General MicrobiologyCourse Code: ZOOL-415 Part - II

MAX. TIME: 2 Hrs. 45 Min.MAX. MARKS: 50

Q.2 . Give short answer of the following1. Sulphur dependent archeobacteria

11. Distinctive characters of algaeiii. Type strainiv. Genetic relatedness and taxonomyv. Distinguish between lithotrophs and organotrophs

Vl. Oxygen toxicity for microbesVll. Nosocomial infections

Vlll. Membrane filter countIX. Chemical composition of bacterial cell wall.x. Animalcules

2x10=20

Q. 3 Write brief note on the following 4 x 5 = 20a. Principal gases that affect bacterial growth.b. Preservation by overlaying cultures with mineral oilc. Arrangements of bacterial flagella.d. Procedure for isolating pure culture.

Q.4 Discuss comprehensively about colony characteristics with the help of 10

labeled diagrams.

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