Thit Aarøe Mørck, LUSH prize 2014
Exposure to environmental chemicals and early markers of adverse effects
- A study using human samples
Thit Aarøe Mørck, PhD student
University of CopenhagenFaculty of Health Science
Department of Public HealthSection of Environmental Health
Outline of today
• Human biomonitoring
• My project
• How I plan to use the LUSH prize money
• Acknowlegdements
Thit Aarøe Mørck, LUSH prize 2014
Human exposure to environmental chemicals
Thit Aarøe Mørck, LUSH prize 2014
Air
Soil
FoodLotions/cosmetics
Toys
Electronics/furnituresWater
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Human exposure with human measurements
Thit Aarøe Mørck, LUSH prize 2014
Chemical exposure
Effects of exposure:Traditional toxicological measurements:- Animal studies- Cell toxicology assays
Biomarkers of exposure-Internal dose
Biomarkers of effect- Early response
Human biomonitoring:
Measurement of total exposure
Thit Aarøe Mørck, LUSH prize 2014
Human biomonitoring
• EU project DEMOCOPHES (DEMOnstration of a study to COordinate and Perform Human biomonitoring on a European Scale)
• 145 mother-child pairs in Denmark
• Two areas (urban and rural)
• Sample collection (urine, hair and blood)
• Questionnaire (mothers)
My project
For at ændre ”Enhedens navn” og ”Sted og dato”:
Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”.Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod
64 biomarkers of exposure and effects
Thit Aarøe Mørck, LUSH prize 2014
Phthalates
Parabens PCBs
PFASs
PBDEs
Paracetamol
Phenols
Mercury
Cotinine
Cadmium
Dioxin-like activity
Micronuclei frequency
URINE BLOOD
HAIR
Micronucleus (MN)
• Biomarker of cytogenetic effects
• Association between high exposure to traffic and MN frequency
• Increased risk of cancer development with increased MN frequency
Thit Aarøe Mørck, LUSH prize 2014
Dioxin-like activity
Thit Aarøe Mørck, LUSH prize 2014
Cell assay measuring the hormone receptor (AhR) mediated activity in serum samples
AIM – use of LUSH prize money
• Associations with exposure measurements and early effects
• Patterns of exposure
Thit Aarøe Mørck, LUSH prize 2014
Phthalates
Parabens
PCBs
PFASs PBDEsParacetamol
Phenols
Mercury
CotinineCadmium Dioxin-like activity
Micronuclei frequency
URINE BLOOD
HAIR
?
Acknowledgements:
Danish Health and Medicines Authority Danish Environmental Protection Agency Danish Veterinary and Food administration
EU projects COPHES and DEMOCOPHES
University of CopenhagenLisbeth E. KnudsenJeanette NielsenJanne Fangel JensenPernille WintonVolkert D. SiersmaLine Mathiesen
Thit Aarøe Mørck, LUSH prize 2014
University of AarhusEva Bonefeld-JørgensenSimon Erdmann
University of Southern DenmarkPhillippe GrandjeanFlemming Nielsen
Free University of BrusselsKim Vande LoockMicheline Kirsch-Volders
LUSH PRIZE ORGANIZATION
For at ændre ”Enhedens navn” og ”Sted og dato”:
Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”.Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod
Thit Aarøe Mørck, LUSH prize 2014
Thank you for your attention
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