Supplemental Fig. 1Live and dead cells estimated by trypan blue cell viability assay in 6-well plates.

0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment

A

0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment

B

* * * *

*p<0.05 in comparison with control

*

* *

d1

d1d2

d2

d3

d3

d4

d4

d8

d8

Liv

e c

ell

s n

um

be

r (x

10

00

0)

Experiment 2

0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5

% DMSO treatment

Experiment 1

d1d4

d6 d7

d8

Liv

e c

ell

s n

um

be

r (x

10

00

0)

0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment

***

*

*

*

*

B’

A’D

ea

d c

ell

s (

% o

f to

tal

ce

lls

)

De

ad

ce

lls

(%

of

tota

l c

ell

s)

*p<0.05 in comparison with control

d1d4

d6 d7

d8