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Sidho-Kanho-Birsha University Ranchi Road, P.O.- Sainik School
District - Purulia, PIN – 723104, W.B.
Phone : 03252- 202419 Website-www.skbu.ac.in
Ref No: FO/324 /SKBU/17 Date: 01/06/2017
E- Tender Notice
1. SIDHO-KANHO-BIRSHA University, Purulia intends to procure Lab Instruments for
Zoology Department at Purulia. The tentative quantity of the required items along
with technical configuration of each items are mentioned at Annexures separately. SKB
University is looking for interested bidders who have experience in supplying of above
type of instruments.
2. Bidders are advised to study all technical and financial aspects, instructions, forms,
terms and specifications carefully in the tender document. Failure to furnish all
information required in the Tender Document or submission of a bid not substantially
responsive to the Tender document in every respect will be at the Bidder’s risk and may
result in the rejection of the bid.
3. Intending bidder may download the tender documents from the website
https://wbtenders.gov.in OR www.skbu.ac.in. Only Online Submission of Tender
will be accepted. Last date of submission: 20/06/2017 up to 12:00 hrs. Opening
Date 22/06/2017, 1 PM.
4. The categories of items and quantity indicated in the Tender Document are
tentative. SKB University (SKBU), however, reserves the right to increase or decrease
the quantity or delete some or all of the items depending on the needs of the SKBU and
availability of funds without assigning any reasons.
5. The bidder should indicate specifically the Basic Price, Taxes, VAT, Entry tax,
other duties (if any), and levies chargeable quantitatively against each item.
University will assist to have way bill (if necessary) but the pay will be borne
by the bidder. University having valid Central excise duty exemption certificate issued
by DSIR, New delhi..No additional information will be entertained after due date. SKBU
may reject bids if they do not carry such information separately and specifically
quantitatively.
6. The tender should be submitted in two cover system (electronically) as defined in E-
Tender participation system: (a) Technical Bid (b) Financial Bid.
7. The bidder should clearly indicate the delivery period and validity period of tender.
8. The bidder should clearly indicate the availability of service and maintenance facilities
at Purulia for the items quoted.
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9. The bidders are required to quote for each item separately in terms of basic price
and all other charges. Prices can be quoted in Multi Currency.
10. The bidder (Technical Bid) must be submitted along with the copies of OEM license
or authority from the manufacturer.
11. SKB University reserves the right to reject any or all tenders without assigning any
reason whatsoever.
12. No advance payment or payment against Performa invoice will be made. Payment
will be made after receipt, inspection, and installation/testing.
13. All damaged or unapproved goods shall be returned at the bidder’s risk and cost
and the incidental expenditure thereupon shall be recovered from the concerned party.
14. On acceptance of tender, the date of delivery should be strictly adhered to
otherwise, the SKB University reserves the right not to accept the delivery in full or in
part. In case the order is not executed within the stipulated period, the SKB University
will be at liberty to make purchases through other sources.
15. Payment of bill will be made through by crossed account payee Cheque or electronic
payment (NEFT) only after delivery and successful installation of each of the items.
16. Delivery Schedule: The Company shall be able to deliver the required items within
15 days of the receipt of order. Delivery/Installation is to be done at Purulia, West
Bengal.
17.Warranty : All the products must carry minimum one year comprehensive warranty.
18. The products asked for should be of very high standard and of mentioned brand.
19. Liquidated Damages : The Company shall be liable to indemnify the SKB
University in all respects and meet and pay off the litigation expenses and all the
liabilities including damages, sums etc. arising out of and as a consequence of the
negligence, deficiencies, mistakes, lapses, delays etc. in the execution of the various
jobs and the services provided.
20. The company should ensure quick back up response in case of equipment failure
which should be replaced if needed within 48 hours of the distress call.
21. Payment: There is no provision for making advance payment to the Company.
However, the running bills for the jobs completed can be submitted by the company
and will be cleared for payment within reasonable period.
22. Purchase Order: The purchase order for the entire quantity can be placed either
in one lump sum or as per the requirement through repeat order subject to availability
of fund of the required items. The quantity shown is tentative and may increase or
decrease.
Registrar
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Annexure 1
Proforma For Techncial Bid
SL Particulars Information
1 Name of the Firm & Owner : (with Telephone/Mobile Numbers, email)
2 Type of Organisation (Proprietorship/Partnership/Pvt. Ltd./Limited Company) Month/Year of Establishment
3 Website of Bidder, if any
4 Month/Year of Establishment
5 PAN/Sales Tax/VAT Regd. Nos.
6 Annual Turnover 2016-17
7 Whether product(s) offered by the bidders are exactly as per the configuration of SKB University. If not, indicate the changes in each product
8 Clientele (submit copies)
9 Validity of Tender
10 Whether Terms & Conditions issued by SKB University are acceptable to the Firm
11 Whether Warranty as per the desired specification
** Bidder should only quote rate for 1 unit of each item in BOQ. Date Name: Signature of Owner/Authorized Representative Designation:
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Technical Specification Technical Specification For e-tender
SL
NO Item Specification Quantity
1
Bench Top Compact High
Speed cell sorter and Flow
based analyzer
1. Bench top flow cytometer Cell Sorter required with 488 nm
/equivalent blue Laser, 633-642 nm /equivalent Red laser and
405 nm / equivalent Violet Laser.
2. The system should be based on cuvette based fixed aligned cell
sorting to avoid any user level alignment while day to day run
of the instrument.
3. The system should have at least 8 fluorescence/colour (10
parameters) measurement capability simultaneously,
upgradable to more colours.
4. System should have minimum3 beam spots without any
customization with the base instrument.
5. The system must have nozzle of size of 100 microns and the
nozzle tip can be removed during operation, replaced and
stream optimized.
6. No manual alignment of nozzle to be done by the user even after
removal and replacement of the nozzle into the system.
7. The system should be able to acquire & sort at least 25,000 or
more -events/second.
8. The system should have option of automatic cell deposition unit
which allows for slide and plate sorting into 6, 24, 48, 96 and
384 plates.
9. Viability & yield should be more than 90% in routine
applications
10. Date Management system : Latest PC Workstation from source,
with compatible monitors
11. The company should offer the latest and updated model and
should have a dedicated training centre with documented proof
of conducting regular training for research applications.
12. The system should be space saving and compact model
13. System must have upgradability option with Bio-safety cabinet.
.
14. Warranty: 1 year from the date of installation
15. Starter Kit for installation and 3 KVA Online UPS & colour
laser Printer should be supplied with the system.
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16. System should be installed with minimum 3 years of
comprehensive maintenance contract ( CMC) from
manufacturer.
17. Minimum 40 lt of sheath fluid should be provided at the time of
installation.
18. At least 6 Fluorescence labelled antibody of choice must be
included in the package.
2
Mini- 1-D vertical gel
electrophoresis system.
The cell should be durable, versatile, and easy to assemble,
and run up to four precast or handcast polyacrylamide gels.
Runs 1–4 mini polyacrylamide gels in 30 min
Leak-free electrophoresis and gel casting
I moulded polycarbonate construction
Ability to run either precast or hand cast gels
Gel size 8.6x 6.8
Buffer volume for 4 gels-1000ml
3
3
Universal power supply
system for horizontal DNA
gels electrophoresis and
vertical acrylamide gel
electrophoresis of proteins
. Should have simple programming and a compact, stackable
case. timer control, constant voltage or constant current output,
real-time monitoring of both voltage and current, and
pause/resume run capability.
Runs four cells simultaneously
Timer controlled
Fully programmable
CE and EN-61010 safety compliant
Case stackable
Voltage10-500V
Current -0.01-2.5 Amp
Power-1 -500W
3
4
Mini wet transfer system
Transfer two 10 x 7.5 cm gels simultaneously
High field strength for rapid 1 hr transfers
Should be able to run overnight at low voltage
Wire electrodes should be placed at least 4 cm apart for strong
electrical fields and efficient protein transfer
Colour-coded cassettes and electrodes for proper orientation of
the gel during transfer
Should have Ice cooling unit, completely contained within the
Mini Blot cell, for absorbing heat generated during rapid
transfers
Buffer-450ml
2
5
Bench-top high speed mini
centrifuge
Capacity: 24 x 1.5/2 mL
Max speed: 13,000 rpm
Max RCF: 17,000xg
Type: Benchtop centrifuge
Run time: 1 to 99 min
Temperature range: Ambient temperature
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6
Cyto centrifuge system
Should be bench-top
Rotor should be able to accommodate 12 glass microscopic
slides.
Should be able to carefully controlled centrifugation to
separate and deposit a thin layer of cells on slides while
maintaining cellular integrity.
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7
Magnetic Stirrer
Speed: 100 to 1700 rpm (Constantly adjustable)
Maximum stirring capacity: 10L
Temperature control: room temperature to 199°C
(Constantly adjustable)
Highest temperature: 300°C
4
8
Water Bath
Specifications:
Capacity: 6, 10, 20L
Heating temperature range: 5°C to 100°C
Temperature accuracy: ±0.2°C
1
9
Vortex
Specifications:
Variable Speed Control
Maximum Speed: 3000 RPM
Choice of Continuous & Touch Mode
Rubber Feet
4
10
Dancing Shaker
Specifications:
Three dimensional combinations of rocking and orbital
motion,
Non slip platform: adjustable from horizontal to a steep angle
continuously,
Low foaming agitation and uniform mixing capacity at slow
speed,
Speed limit: 0-60 RPM.
4
11
PH meter
Microprocessor based, Ph range 1-14
Digital display, touch based calibration and measurement
button. systems that will measure pH, ion concentration (ISE),
conductivity, dissolved oxygen, temperature, or a combination
of parameters.
4
12
CO2 Incubator Co2 incubator for mammalian cell culture, temp range 37
degree, chip based temperature and CO2 control. 3 rack, outer
body corrosion proof, should have UV for sterilization.
1
13
Micropipette
Fully autoclavable, 0.2 -10 ul, 2-20ul, 20-200ul, 200-1000ul
and 5000ul
3 each
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14
Multichannel pipette
Should have 12 channels, 20-300 ul.
2
15
FLUORESCENCE
SPECTROPHOTOMETER
FEATURES:
System must have a built in high resolution large LCD
display and should have provision for PC communication.
Microprocessor controlled Fluorescence spectrophotometer
must be effective for 3-D measurement and realtime
auto-scale function with a unique high-efficiency diffraction
grating.
A small amount of 1 x 10-12 mol/L fluorescein should be
detected by the low fluorescence intensity of range from
0.001 to 9999.
Should have option for quantum yield measurement.
System should be satisfied Cutting-Edge Fluorescence
analysis.
Should have automatic pre scan and compatible for GFP
measurement and DDE, OLE functions.
The dynamic range Should be at least 6 order magnitude or
more
Should have a JIS K 0120 satisfied performance check
function.
Should have monochromatic light monitoring ratio
calculation function.
SPECIFICATIONS:
Measurement techniques : 3-dimensional Fluorescence measurement, automatic sensitivity measurement, measurement of wavelength accuracy and wavelength setting repeatability. prescan, Data storage capacity(minimum up to 50)
Spectral Range : Should 220-730nm, with 0-order light Extended up to 800nm , with optional PMT detector
Spectral Bandwidth : 2.5, 5, 10, 20nm (Both excitation and emission)
Sensitivity : High sensitivity with S/N 10000 or better (RMS) using Raman band of water (Peak to Peak) Excitation wavelength 350nm, bandwidth 5 nm, response 2s.
Light source : Self-deozonating Xenon flash lamp 150 W
Detector : High Performance Photomultiplier Tube(R928F)
Monochromator : Aberration-corrected stigmatic concave diffraction mechanical grating instead of Holographic grating Brazed wavelength : Excitation side 300nm,(emission side) 400nm (fluorescence side). Half value width calculation with spectrum averaging
Wavelength pre scan speed : Should 1500nm/min.
Wavelength drive speed : Maximum 12000 nm/min
Wavelength Accuracy : ±2 nm
Wavelength Repeatability : ≤ 1nm
Photometric value range : -9999 to 9999
Wavelength scan function : Provision for synchronous spectra/repetitive measurement/CAT, Linear to
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biquadratic differentiation, trace, scale conversion, smoothing etc
Working temperature / humidity : 15 to 35ºC, 45 to 80% (condensation not allowed, 70% or less at 35ºC or higher)
Dimensions / weight : Spectrophotometer: 620 W x 520 D x 300 H mm (excluding protrusions)/ 41 kg
Software should have provision for controlling the instrument & its accessories & 3-dimensional time scan measurement and Data processing features such as quantitative analysis, cumulative data averaging, first to fourth order differentiation, statistic calculation, graph axis change, smoothing, area calculation ,spectrum normalization ,wavelength scan measurement, time based measurement such phosphorescence life time, 3-D measurements, Data export to Microsoft Excel etc.
All the above specifications should be mentioned in printed
literature of manufacturer
Company should have factory trained engineer & Kolkata
based service station.
16
ICE FLAKING MACHINE
WITH BUILT-IN-BIN
Microprocessor controlled fully automatic Ice Flaking
machine.
Air cooled system
CFC Free Refrigeration system.
Ice faker body is made of non corrosive steel and well
electrically grounded for safety.
Auto shut off if water temperature is high
Built in visual alarm
Stainless steel insulated storage bin.
Auto shut off when bin is full.
Auto shut off of low water pressure
Continuous crushed ice production facility.
Electronic controlled system.
Highly hygienic bin for storage of ice.
Low power and water consumption.
Attractive drain hose
Noise free compressor
Production capacity : 70 kg /24hrs
Bin capacity : 25 kg
1
17
ULTRA SONICATOR
SYSTEM
Compact ultrasonic processor
Power: 200 Watts
Frequency : 24 kHz
Capacity: Up to 2000 ml.
Automatic frequency scanning system,
Amplitude adjustable 20-100%, pulse adjustable 0-100%.
Dry running protection.
Solid titanium probe for all sample even for organic solvents
or low surface tension liquids. Replaceable tip or sonotrode
with multiple joint will not be entertained.
Easy positioning of samples under the ultrasonic probes to
control immersion depth, stainless steel.
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18
Inverted Microscope with
Bright Field, DIC
&Fluorescence
Stand ergonomically designed, rigid and shock proof. Controls
for light path selector, transmitted light intensity and ON/OFF
switch.
Bright Field, DIC and Fluorescence
Universal/Long working distance condenser with 6/7 position
and with minimum working distance of 27-28mm
Coded nosepiece for easy operation, Features must be high
grade smooth operation and with positive click stops
Manual XY Stage with universal sample holder for plates,
glass slides, petri plates etc.
The microscope should be provided with a DIC slot, a
polarizer and an analyser. It should have complete DIC
accessories for the objectives specified.
10X/22mm field of view or better
LED illumination with minimum life of 40000hrs
Binocular type observation tube
Plan Objectives 5x, 10X (NA 0.25), 20X (NA 0.40),
Fluorescence grade CORR Objective 40 X (NA 0.5-0.6mm)&
Fluorescence grade 100x (NA 1.3)
Coded filter turret with 6 or more position.
Long Life cold LED Light Source with minimum 20000 Hrs
life span
Filter for DAPI, FITC, Rhodamine
Cooled fluorescence colour camera with USB3.0 interface.
Ideally suited for both- low light fluorescence application and
brightfield imaging with superb colour reproduction. Colour
CCD sensor with desired technology to offer exceptionally
high speed of acquisition combined with high sensitivity even
in the near infrared region. The camera should have minimum
1920 x 1440 pixel, 4.5 µm x 4.5 µìm pixel size and a typical
full well capacity should be minimum of 15.000 electrons.
Partial scan, various binning modes (color and monochrome),
and overlapping mode for high-speed imaging should be
available.
Max. Frames per second should be minimum of 40 fps in full
frame. Suitable C-mount for Side Port should be. The camera
make should be from the same manufacturer as that of the
Microscope.
Image capture and analysis software and should include It
should have multi-channel fluorescence capturing and
merging, point to point measurement, annotation and scale
bar.No third party Software for imaging system will be
accepted.
The Microscope should be accompanied by an Imaging
/Computer system having a configuration of no less than Intel
i5 processor, 4GB RAM, 1TB HDD, minimum display 18
inch.1 KVA UPS should also be provided for the system(s).
Warranty of at least 1 year from the date of installation
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19
GCMS
GC:
1. Oven temp. Upto 4500C
2. Injector port temp. Upto 4500C
3. AFC pr. Range: 0 to 970 Kpa
MS:
1. Type: Direct connection with capillary column 50
2. Temp: to 3500C
3. Ion source temp: 140-2600C
4. Filament: Dual (Automatic switching)
5. Electron energy: 10-200 eV
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6. Emission current: 5 - 250µA
Mass analyser and Detector:
1. Mass analyser: Metal quadrupole mass filter with pre rod
2. Mass range: m/z 1.5 – 1000
3. Mass resolution: 2M (FWHM)
4. High speed scan rate: 10,000µ/sec
5. Minimum measurement interval: 0.01s (maximum
100scan/sec)
6. Detector: Secondary electron multiplier with the patented
overdrive lens and conversion dynode.
7. Main Pump: Turbomolecular pump (58L/sec for He)
8. Force Pump: 30L/min (60Hz) Rotary Pump
9. Column Flow: upto 4mL/min (He)
Soft ware (GCMS soln. Ver 2.6)
Mode- Scan, SIM, FASST
SIM-64 channels x 128 group
Energy saving- Ecology Mode
Method creation: COAST (Creation automatic SIM Table)
AART (Automatic Adjustment Retention time)
Instrument tuning: Autotuning for EI modery of
Library of compounds for identification and matching .
20
Water Flow meter (Digital)
Nominal Dia (mm): 15-400
Electrode material: MS, SS 304, SS316
Process pressure-Max 25kg/cm2
Flow tube material: SS 316
End connection- Flange
Measuring range-0.5m/sec for minimum and 5m/sec for
maximum
Display- 2 Line Alfa Numeric LCD
Display Unit- Standard Unit in m3, lit
Power supply: 230VAC, 110VAC
Output: Std 4- 20mA
Protection clas for sensor- IP 65
Protection clas for transmitter-IP 65
Cable length for remote- 10mts
Installation – Inline Flange type
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21
Refrigerated Bench Top
centrifuge:
Control: Microprocessor
Capacity: 4x400ml
Max, RCF: 25830xG
Max speed: 15,200 rpm
1
22
Amino acid analyser:
Column: 4.6mmD, 60mmL
Pump: Delivery pressure 0 to 20 MPa
Flow rate range: 0.000 to 0.999 mL/min.
Auto sampler: Injection method- Direct injection method
Sample vial capacity- 1500µL
No. Of vial accommodated- 200
Sample injection Vol: 0.1 to 100 µL
Column Oven: Temp. Setting range: 20-850C
Photometer: Spectrophotometer. A planatic concave
diffraction grating
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23
Portable Dissolved oxygen
meter:
1. Polarographic measuring system
2. Automatic calibration
3. Auto temp. Compensation-0-500C
4. Altitude compensation: 0 to 4000mts with a 100mt.
Resolution
5. DO range: 0 to 45 mg / l
6. DO resolution: 0.01mg/l
7. Temp. Range: 0 to 500C
8. Salinity compensation: 0 to 80 g/l
9. Electrode /Probe: Polarographic DO probe
10. Battery type: 1.5 VAAA(3)
11. Dimensions: 185x72x36mm
12. Wt. 300g
1
24
Fully Automated Rotary
Microtome
1. Section thickness setting: 0.5 – 100 µm
2. Trimming section thickness setting – 1 – 600 µm
3. Vertical stroke – 70 mm/2.76 inches
4. Reposition of knife holder base:
5. Horizontal: ±24mm/0.94 inches
6. Vertical: ±23mm/0.91 inches
1
25
Upright trinocular research
microscope with
transmitted light bright
field, phase contrast &
Fluorescence & DIC
application along with High
resolution color CCD
camera & software
1) Microscope should be suitable for Bright Field, Dark Field,
Phase Contrast, Fluorescence & DIC application. Also should
be dual camera attachment facility.
2) Transmitted light halogen lamp for 12V/100 watt for
observation and photomicrography under bright field & phase
contrast application, DIC application along with a spare lamp.
3) Binocular phototube with dual camera facility should be 20º
for better ergonomic & (100:0/0:100) for observation under
bright field, fluorescence & DIC illumination.
4) Widefield focusable 10x eyepieces with larger field of view
should be 23 -25 mm or higher for observation under
fluorescence & DIC image.
5) 6x revolving nosepiece (accommodate 6 objectives at a time).
6) Infinity color contrast corrected optical system based objective
5x, 10x, 20x, 40x, 63x, 100x (oil)
should be Fluorite objective with DIC facility & 100x should
be high N.A 1.30.
7) Magnification changer 1.25 x / 1.6x / 2.5x / 4.0x should be
available with push and click module. Fluorescence & DIC
images as well as photographic images can be magnified.
8) Achromatic aplanatic universal turret type condenser 0.9 for
for bright field, Phase contrast & DIC application
9) Large circular disc type Intensity regulator & focusing knob
placed both side ,accessible from both side.
10) Microscope stand should be built in 6-position filter wheel
equipped with filter slider at the back for -Interferance green
filter, white balance filter, contrast enhancing blue filter,
Neutral Density filter 0.25 & Neutral density 0.06, daylight
conversion filter for contrast enhancement for transmitted light
application.
11) Fluorescence reflector turret should be six position for
fluorescence filters.
12) The burner should be removable with the drawer – for a
particularly easy and comfortable change of burner.
Fluorescence Filter with patented stray light trap system. filters
are insertable and removable with push & click method
without any need of tools.
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100 watt mercury vapour lamp for fluorescence illumination
with fluorescence filters
Fluorescence Filter Excitation shift free EX BP 450-490,
Emission filter LP 515
Fluorescence Filter Excitation BP 546/12, emission LP 590
Fluorescence Filter UV Excitation shift free EX G 365, with
antiglare screen
Photography system:
Basic CCD resolution 6.0 MP, pixel pitch should be 4.65µm X
4.65µm or better,
Chip size: 12 mm x 10 mm, equivalent to 1" (16 mm
diagonal), monochrome CCD,
Spectral range: with protection glass app. 400 nm to 1000 nm,
binning upto 5x5
Live frame rates (depending on hardware and software
configuration):
Exp. Time 250 ms to 60s, Frame Rate 51 fps, Cooling: One
stage Peltier cooling, sensor temperature
Digitaization 14 bit/pixels, image sensor 2/3”, live image
frame rate 15 max. (on resolution upto 1920 x 1080) or better,
exposure time: 1 ms to 4s, dynamic range:- approx. 1:500 &
imaging software for live image preview, multi channel
fluorescence, multi focusing module, interactive measurement,
time lapse, shading correction, color adjustment, white balance
settings, text annotation etc complete with CCD C-mount
adapter 0.63x compatible trinocular microscope completed
with a suitable Laptop/Desktop (Lenovo/Dell/HP with Intel
core processor, 6th Gen, 8 GB DDR4, 1TB HDD, 2-4 GB
graphics card, 14-15 inch screen) to supplied along with the
system to ran the CCD camera & software
2626
26
Trino Inverted Cell Culture
Microscope for Bright Field
& Phase Contrast with
photography system
1) Inverted Microscope with 6V/30-35 watt halogen illumination
as well as long lasting LED
lamp attachment for observation and photography under
bright field & phase contrast.
Microscope should have binocular phototube 45°/20 to attach
the camera.
Provision to upgrade for LED Fluorescence application in
future.
2) High quality Infinity Corrected System Objectives 10x Ph1,
20xPh1 and 40x Ph1, 100x dry and wide-field paired eyepiece
10x /20 mm FOV with focusable & dioptre adjustable
swivelling siedentopf tubes for comforting viewing angle.
3) Inter Pupillary Distance adjustment range 48-75mm. Intensity
settings to be easily displayed on both sides of the stand by
live LEDs as used as intensity indicator.
4) Large Specimen stage 200 mm x 239 mm to accommodate
different samples, large tissue culture bottles, petri dishes,
microtiter plates and culture flasks. Stage enlargment
accessory available. XY movement guide for stage and
holders for petridishes, 96 well plates should be available.
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5) Should be completed with filter for even homogeneous
illumination & Interference green filter for phase contrast
sample. Long working Condenser 0.4 with universal phase
slider for phase objectives & built-in aperture diaphragm for
bright field and phase contrast.
6) There should be automatic stand-by for illumination/ on/off
switch facility of light illumination ideal for 10-15 min. to
increase the life time of the illumination
7) Resolution upto 5.0 Megapixels & pixel size should be: 2.2
µm x 2.2 µm or higher, frame Rate maximum 38 fps &
complete with c-mount adapter
Imaging software for image capturing, live image preview,
white balance adjustment, interactive measurement, angel,
cantour, acquisition & annotations.
1) 27
2) Accessories for Upgradation
for with PlasDIC, Objective,
Imaging system for existing
Existi 'Carl Zeiss' Inverted Microscope
“Axio Vert A1-FL
Microscopy Camera Axio Cam ICc5 (Make: Carl Zeiss)
With AxioVision driver, configuration tool and connection
cable firewire CCD Sensor, 5.0 Megapixels, color, CCD,
Pixel size: 3.45 µm x 3.45 µm, Zeiss Axio vision Imaging
Software - Interactive measurement: length, contours, angles, -
Geometric scaling , Measurement bar, - Management,
visualization and printing of metadata - Image processing and
optimization (contrast, brightness, gamma, colors), Colour
adjustment, White balance
PlasDIC module LD A-Plan 10x-63x for contrast slider 10x29
mm
3 position contrast slider 10x29 mm for PlasDIC module and
analyzer
Analyzer module Pol ACR P&C for transmitted light
Slit diaphragm 3.5 mm PlasDIC for condenser (10x-40x)
Slit diaphragm 5 mm PlasDIC for condenser (40x/63x)
Slider PlasDIC for LD A-Plan 10x-63x
Analyzer module Pol ACR P&C for transmitted light
Objective EC Plan-Neofluar 100x/1.30 Oil M27
(WD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover
glasses, high performance, D=0.17mm, box with 100 pc.
Objective LD Plan-Neofluar 63x/0.75 Corr Ph2
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28
HIGH SENSITIVE
CONFOCAL
WORKSTATION WITH
HIGH RESOLUTION
ATTACHMENT
cell biology applications, cell lines, tissues. The system should
be available with the below mentioned configuration as
mentioned below:
A. Motorized Inverted Fluorescence Research Microscope:
a) Fully Motorized Inverted Fluorescence Research Microscope
for BF/DIC/Fluorescence preferably with dedicated touch
screen TFT display for controlling motorized components of
the microscope.
b) Mot. X-Y stage with Universal sample holders for slides, 35/60
mm Petri dish, labtek chambers with multipoint, tile and mosaic
imaging software.
c) LED / Halogen llumination for transmitted light & 120W metal
halide illumination for Fluorescence should be offered.
d) Motorized 6 position DIC nosepiece, Universal Motorized
Condenser NA 0.55 or better with modules for DIC, 6 position
fluorescence turret for accommodating fluorescent filters for
sample visualization.
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e) High precision Z-focus drive with step size of 15 nm or better.
f) High resolution confocal grade objectives of 10x/0.40,
20X/0.80, 40x/1.30oil, 60/63x/1.40oil immersion.
g) Automated shift free DIC accessories for all objectives.
h) Band pass fluorescent filters for DAPI, GFP, Cy3 should be
offered.
i) An active anti-vibration table with compressed air damping,
bread board table top with M-6 threading for the complete
microscope system.
B. Spectral confocal imaging unit with built-in high sensitive
detectors:
a) Laser point scanning and Confocal detection unit with 3
channels for simultaneous detection of 3 fluorophores.
Detectors should be capable of working in Intensity and
Spectral mode Imaging. System should be a combination of min
2 PMT + 1 GaAsP/HyD detectors in the scan head.
b) Scanner unit should have laser ports for 4 lasers to be integrated
with the system.
c) The scanner should have real"ROI" scan capability for fast scan
. Maximum scan resolution should be at least 4Kx4K or better
per channel and should reduce to 16X16 resolution.
d) Scan speed should be 7-10 fps or better @ 512x512 and should
be able to increase up to 50 fps@512X32 or better.
e) Transmitted PMT for laser based DIC imaging should be
included.
f) The scan field diagonal should be min 18 mm or better. Scan
Zoom range minimum 1X to 40X with increments of 0.1X.Scan
rotation of 360 degrees should be available.
C. Solid State Laser module with AOTF control:
a) 405 nm,
b) 488 nm
c) 561 nm.
d) 640 nm.
All lasers should be connected to the scan head through fiber
optic cable and should be controlled through AOTF for fast
laser switching and attenuation in pixel precise synchronization
with the laser scanner for Real ROI scan for FRAP, Photo
activation/conversion experiments. All the laser lines should be
controlled through a computerized AOTF device for fast laser
switching and attenuation.
D) High Resolution Imaging: a) Real-time and Online Fully automated and motorized HR
attachment with suitable high sensitive Detector for complete
Vis Spectrum.
b) Lateral resolution of 150nm or better and Axial resolution of at
least 400 nm or better should be expected out of the system.
c) Detection should be based on GaAsP or high sensitive
detectors.
d) Should be able to perform live cell SR Imaging . All laser lines
for Confocal Imaging should be used for imaging in SR mode.
F) Control computer and Monitor:
Latest 64 bit control computer with Intel Xeon Processor,
DDR RAM 32 GB HDD: 2TB SATA upgradable to 4 TB or
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better, DVD, SuperMulti SATA +R/RW, Graphics : AT Fire
GL V5200 256MB DH DVI, Gigabit Ethernet, Win 7 Ultimate
64 bit , USB 2.0, Fire wire. Large 30” LCD TFT monitor.
G) System control and Imaging Software:
a) Software should be capable of controlling Motorised
components of microscope, digital camera, confocal scan head,
laser control including AOTF and Image acquisition &
processing for confocal and super resolution imaging.
b) Saving of all system parameters with the image for
repeatable/reproducible imaging.
c) Line, curved line, frame, Z-stack, Time series imaging
capabilities.
d) Real ROI bleach for FRAP, Photo-activation/conversion
experiments.
e) Standard geometry Measurements like length, areas, angles etc
including intensity measurements.
f) Advanced 3D image reconstruction with rendering from a Z-
stack image series.
g) Co-localization and histogram analysis with individual
parameters.
h) Spectral un-mixing with fingerprinting for separation of
overlapping excitation/emission spectra of fluorophores.
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Compact
chemiluminescence and
fluorescence gel and
western blot documentation
platform
full-feature instrument for imaging and analyzing gels and
western blots. Should be designed to address multiplex
fluorescent western blotting, chemiluminescence detection,
general gel documentation applications, and stain-free
technology imaging needs.
Automatic recognition of application-specific tray and
adjustment of imaging parameters and software options
Precalibrated focus for any zoom setting or sample height
Dynamic; precalibrated and optimized for each application
Mage area: 21 x 16.8 cm
Cool CCD camera, 6 MP
Trans-UV, 302 nm excitation
Epi-white
Trans-white (with White Sample Tray)
Trans-blue, 450−490 nm excitation (with Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
590/110 nm standard filter to perform protein and DNA gel
and blot imaging
Chemiluminescence filter
518–546 nm filter for green-emitting fluorophores and stains
577–613 nm filter for yellow/orange-emitting fluorophores
and stains
675–725 nm for red-emitting fluorophores and stains
700–730 nm filter for far red–emitting fluorophores and stains
813–860 nm for near IR–emitting fluorophores and stains
1
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Portable microweighing
Balance
Specifications:
Capacity: 120g-10 microgram
Minimum display: 0.01mg
Pan size (mm): 110 dia
Power supply: two ways (AC or Battery operation).
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Balance Specifications:
Capacity: 32/180 Gms
Readability: 0.01mg
1
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Cryotome
Specifications:
Temperature Control Chamber: 0° to -35°C (at 20°C
ambient temperature)
Microtome: Specimen retraction 25,000 μm
Microtome: 1-20 μm (1 μm increments), 20-60 μm (5 μm
increments)
Ordering Alerts: XY orientation head is standard
Specimen temperature control, debris tray, Storage shelf,
Cryocassettes
1
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FULLY AUTOMATIC SOLVENT
EXTRACTION WITH HYDROLYSIS SYSTEM
Total Fat System: The system should be offered for Total Fat
solution which automates and simplifies total fat analysis. Consisting of an extraction unit, a hydrolysis unit and a single filter system that is common to both units, the Total Fat solution should perform Acid Hydrolysis and Solvent Extraction with one integrated procedure. The system should reduce the potential for human error in total fat analysis while reducing costs and improving overall sample throughput.
Extraction Unit: Fully automatic 6 sample solvent extraction system for Oil/ Fat extraction with 4 stage extraction using Randall procedure viz., Boiling, Rinsing, Solvent recovery and pre-drying steps. 6 sample extraction unit should have facility to expand to 12 position extraction unit & should be controlled by same control unit.
Extraction positions should have individual hotplate control.
Must be fully pre-programmable. Can set and store temperature and times for various steps.
Official approvals: AOAC, SCAN-CM, EPA
Fully automatic for unattended operations
Fully autoclavable liquid handling drive of 100ul to 5ml ( 4 ranges )
1
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Aerosol barrier protection device of 10ul/20ul/100ul/200ul & 1000ul (96x8)
Stirring motorless upto 1.5K capacity 1500ml
System should have Hydrocap filter which contains the sample from the initial weighing through hydrolysis to final extraction avoiding sample transfer errors. It should be a fully automated system that performs addition of acid, heating, boiling , rinsing and draining.
The extraction unit should have a speed dial for closed solvent addition for safe and consistent solvent addition so avoiding manual handling of chemicals.
The extraction unit should perform the four extraction steps boiling, rinsing, solvent recovery and auto shut down, fully unattended.
System should have extensive range of accessories with glass and aluminium cups and thimbles in different sizes to fit individual laboratory needs and applications.
System should quote with accessories comprising: 3 sets Extraction cups glass & Aluminum, standard 6/set each, 2 Thimble stands 26-33mm, 2 sets of Thimble adapters plastic 33mm 6/set, 1 set of Cellulose thimble 33 mm single thickness 25/set, Condenser Seal Viton/Butyl 6/set, Condenser Seal Resel 6/set, Condenser Seal Polyurethane 6/set
Minimum quantity of solvent required per extraction is 70 ml to 90 ml.
80% of solvent is recoverable at the end of the cycle.
Extraction time typical 45 min to 60 min . Extracting a batch should not be more than 60 minutes.
Capacity per batch should be 6
Capacity per day should be 42
Measurement range should be 0.1 to 100 %
Sample Size 0.5 gm to 2 gm ( for total fat)
Reproducibility should be +/- 1 % rel. or better ( 5 – 100 % fat)
Accuracy should be according to officially approved methods
Instrument should have 9 different programmable capacity
Power rating should be 1800 Watts
Temperature range 0 to 285 deg Cel. & heating up time for 20 – 285 deg Cel should be 5 min.
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Instrument should have Cooling water tab & should have water
flow rate 2 lit/min for < 25 deg Cel.
Ventilation: Fume hood. Mains power interlocked to the airflow which must be at least 0,5 m/sec.
Protection class should be IP 41
Batch handling tools for loading of solvent cups and thimbles are to be provided with the instrument.
Addition of solvent should be using a closed system, to eliminate hazard in the laboratory.
A safety door in front of the extractors is essential to ensure laboratory safety while using solvents.
Over temperature protection should be given at different levels of 145, 210 and 330 deg. C which can be selected depending on the solvent used.
34
Accessory for existing gel
doc system
Monochrome CCD Camera
16 bit 1.4 mega pixel resolutions and 4.2
megapixel image resolution.
Low noise. High speed USB data interface
Peltier Thermoelectric Cooling to -28 d.c. below
ambient
Pixel Size : 4.65µm x 4.65 µm
Full Well capacity : ~20000 e-
Read noise : <5 e- rms
Dynamic range : 4 orders of magnitude.
1
35
Kjeldahl Digestion Unit
Amperage- 11A
Depth (English)- 18.25 in.
Electrical Requirements- 115V 50/60Hz
Height - 43cm
Width- 26cm
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36
1. Furnace System
(for Upgradation of Thermofischer AAS)
Furnace head
Furnace power supply
Furnace auto-sampler
Fixed mount
Normal Electrographite cuvettes (10)
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37
2. Semi auto Biochemical Analyzer
(Blood Biochemistry)
Method: Kinetic, End-point, Linearity end-point,Non-
linearity end-point,
Absorbency: Sampl blank, Multi-
standards, Velocity, Calculation, Fixed
Time, bichromatic etc.
Flow cell: quartz flow cell 32uL
With both shipping and cuvette system
Lamp: 6V 10W
Measurement range: 0~2.5Abs
Resolution: 0.001 Abs(display), 0.0001 Abs(calculation)
Temperature control: Peltier 37°C,30°C,25°C ± 0.1°C
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3. Ball Mill
With grinding bowl and balls
Number of working stations 2
Grinding bowl sizes 20, 45, 80 ml
Max. feed size (depending on the material) 5 mm
Min. sample quantity 0.5 ml
Max. sample quantity 70 ml
Final fineness (depending on the material) < 0.1 µm
Typical grinding time (e.g. quartz sand to < 40 µm) 4 min
Grinding process Dry/wet
Speed of main disk 100 – 1100 rpm
Transmission ratio i relative = 1 : –2
Effective diameter of main disk 140 mm
Centrifugal acceleration 95 g
1
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Interfaces USB, Bluetooth, Ethernet
Electrical details 100-240 V/1~, 50-60 Hz, 1200 watt
Bowl size- 20 ml
Ball diam- 5mm
39
BINOCULAR LABORATORY MICROSCOPE
Body: Aluminum die-cast body with all critical movements based on ball bearing & wire guides thereby ensuring smooth & precise manipulation Optical System: Optics par focal, infinity plan achromatic lenses with anti fungal properties and upgradable to EPI LED fluorescence. Illumination: • Built in transmitted Koehler illumination. • 6V30W Halogen illumination • work on voltage 100 V-240V AC. Focusing: Co-axial coarse & fine controls with a focus adjustment and fine adjustment knobs. Coarse Focus range 20mm. Fine focus range 0.2mm Revolving nosepiece: Quintuple revolving inward nosepiece based on precision ball-bearing mechanism with positive click stop Mechanical Stage: Co-axial low drive mechanical stage(125mm x145mm)(+/-5mm)with traverse area of 50mm x 76mm (+/-5mm) with dual side holder Condenser Holder: Rack & pinion mounted condenser holder Condenser: Center able abbe condenser with aperture iris diaphragm (N.A. 1 .25) focusable with rack & pinion through 20mm and a continuously variable iris diaphragm with a removable blue filter for day light observation. Objectives: Plan Achromatic Objectives N.A W.D 4X 0.10 25 mm 10X 0.25 5 mm 20x 0.40
12
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40X (spring loaded) 0.65 0.5 mm 100X (Oil, spring loaded) 1.25 0.14 mm lnfinity corrected plan optics Uniformly centered, interchangeable & Parfocal Anti fungus treated Tropicalised anti fungus treatment ensures image excellence for long periods in conditions favoring to fungus growth Inclined Observation Head: Binocular (30 degree inclined seidentopf), 360 degree rotatable, diopter adjustment Eyepiece: WF 10x (FN 20mm) paired eyepiece. The unique optical design of the compensating eyepiece provides relief from eye fatigue and renders color-compensated wide field images of utmost clarity.
Dr. Nachiketa Bandopadhyay Registrar
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