Presented by: Mike McKenna
Setting Up Your Cannabis Lab for Potency Testing
•Cannabichromene (CBC)•Cannabidiol (CBD)•delta-8-THC•delta-9-THC•Cannabigerol (CBG)•Cannabinol (CBN)
Analysis of Cannabidiols in Marijuana Plants:
Fig 1: Cannabinoid-StructuresSource: http://blog.restek.com/?p=7704
Three Stages of Cannabis Analysis:1. Extraction2. Analysis 3. Reporting
Discussion Points:
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Sample must be put into liquid phase.•Step 1: Representative Sampling of Plant•Step 2: Sieving Plant Material•Step 3: Extraction or Sieved Plant Material
Extraction of Marijuana Plant Material
GenTechScientific.com Booth #105 Instrumentation - Training - Service
•Cuttings: ▫Taken from all over the plant▫Composited together
Extraction Step 1: Representative Sampling of Plant
Source: www.oregonlive.com
GenTechScientific.com Booth #105 Instrumentation - Training - Service
•Sieving:▫Cuttings are placed in a sieve ▫Shaken until a sized
proportion is collected.
Extraction Step 2: Sieving Plant Material
Source: www.allbud.com
GenTechScientific.com Booth #105 Instrumentation - Training - Service
•Extraction▫Weigh plant material into a vial
or beaker▫Add solvent▫CO2 extraction
Extraction Step 3: Extraction of Sieved Plant Material
GenTechScientific.com Booth #105 Instrumentation - Training - Service
•Gas Chromatography Flame Ionization Detector (GC/FID)•Gas Chromatography Mass Spectrometry (GC/MS)•High Performance Liquid Chromotography (HPLC)• Liquid Chromatography Mass Spectrometry (LC/MS)
Analysis of Extracts
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Gas Chromatographs are used for the Cannabis testing applications, such as for Residual Solvents, Cannabinoid Characterization and THC potency.• Place aliquot of extract into a heated
injection port• Molecules in the gas phase are
separated by partitioning between the gas phase and the stationary phase
• Detected by ionization in the flame detector
Gas Chromatography (GC/FID)
Fig 2: FIDSource: http://blog.restek.com/?p=7704
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Same procedure as Gas Chromatography Flame Ionization but ….• molecules are detected by mass and
mass fragmentation• Most reliable method of identification
Gas Chromatography/ Mass Spectrometry (GC/MS)
Agilent 5973N GC/MSD System with 6890 GC and 7683 Autosampler
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Along with a GC, an HPLC can assist in quantifying the potency of a Cannabis sample. It's possible to accurately determine the levels of the components such as THC, THCA, CBD, CDBA and CBN.• Molecules partitioned between liquid
phase and solid support coated with polymer
• Molecules are not degraded by heat
High Pressure LiquidChromatography (HPLC)
Waters 2695 Separations Module with 2487 Detector
GenTechScientific.com Booth #105 Instrumentation - Training - Service
When testing for residual solvents such as pesticides, water, butane, carbon dioxide, ethanol and other solvents used in cannabis manufacturing, an LCMS will assist in determining their levels.• Same procedure as liquid
chromatography but molecules are detected by mass fragmentation
• Solvent Residue• Pesticide Residue
Liquid Chromatography/ Mass Spectrometry (LC/MS)
AB Sciex API 3200 QTRAP® LC/MS/MS
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Chromatograms that are created are integrated by several kinds of devices.• Integrator•Chromatography Data Stations• LIMS Systems
Reporting of Results of Extracts
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Source: http://blog.restek.com/?p=7704
•Cheap• Labor-intensive•Transcribe results into spreadsheet• Integrator and mathematically corrected results are transferred to final
client report.
Integrator
GenTechScientific.com Booth #105 Instrumentation - Training - Service
• Chromatograms are processed by computer software
• Reports are generated with identification of analytes and concentrations
• Results are then added to a final customer report
Chromatography Data Stations
GenTechScientific.com Booth #105 Instrumentation - Training - Service
•Results are transferred to a computer server where they are manipulated and placed on a final customer report
• Least labor involved
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Laboratory Information Management Systems
Training• We offer customized hands-on training at our location or yours!• Train your team on testing theory, operation of the instrument, how to effectively run samples, and method development.
GenTech Solutions:
GenTechScientific.com Booth #105 Instrumentation - Training - Service
Installation• GenTech offers the option to install and setup your lab. • Also includes operator familiarization training from our
expert service engineer.
• Extraction of Marijuana Plant Material▫Representative Sampling, Sieving Plant, Extraction
• Analysis of Extracts▫GC/FID, GC/MS, HPLC, LC/MS Instrumentation
• Reporting of Results of Extracts▫ Integrator, Chromatography Data Solutions, LIMS Systems
• GenTech Services:▫Training & Installation
Summary
GenTechScientific.com Booth #105 Instrumentation - Training - Service
References: Restek, Marijuana Growers Headquarters & Analytical 360
Thank You from
Booth #105
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