Science Learning Center
Chemistry Pre-laboratory Tutorials
Written By Patrick Herklotz
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Tutorial 3 Calibrating the Vernier Colorimeter Sensor and Recording Data Using
this Sensor
Top View
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 1: Assemble the LabPro Calculator as explained in Tutorial 1
Top View
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 1: Assemble the LabPro Calculator as explained in Tutorial 1
Top View
Previous Slide
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Side View
Channel 1 Channel 2 Channel 3 Channel 4
Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment.
CAL
430nm
470nm 565nm
635nm
Top View
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment.
Side View
Channel 1 Channel 2 Channel 3 Channel 4
Channel 1 Port
click
VERNIER SOFTWARE DATAMATE
(VER 1.15)
ROM 6.27 (C)2003
Checking Sensors
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CH 1: Absorbance 0.475
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
CAL
430nm
470nm
Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected.
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected.
CAL
430nm
470nm 565nm
635nm
CAL
430nm
470nm 565nm
635nm
Top View
Previous Slide
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Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
TI-84 Silver EditionTexas Instruments
CH 1: Absorbance 0.857
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key.
CAL
430nm
470nm 565nm
635nm
Top View
Previous Slide
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key.
TI-84 Silver EditionTexas Instruments
CH 1: Absorbance 0.857
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CH 1: ColorimeterCH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-900
1:OK 3:ZERO2:CALIBRATE 4:SAVE/LOAD
SELECT CHANNEL1: CH1-ABSORBANCE2: ALL CHANNELS
CH 1: ABSORBANCE -0.135
PRESS [ENTER] TO ZERO
CH 1: ABSORBANCE 0
Mode: TIME GRAPH-900
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
Colorimeter is now Calibrated!
CAL
430nm
470nm 565nm
635nm
Previous Slide
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
TI-84 Silver EditionTexas Instruments
CH 1: ABSORBANCECH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-180
1:OK 3:ZERO2:CALIBRATE 4:SAVE/LOAD
Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the “down key” to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen.
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the “down key” to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen.
TI-84 Silver EditionTexas Instruments
CH 1: Absorbance 0.857
Mode: TIME GRAPH-990
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CH 1: ABSORBANCECH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-180
1:OK 3:ZERO2:CALIBRATE 4:SAVE/LOAD
SELECT MODE1:LOGDATA2:TIME GRAPH3:EVENTS WITH ENTRY4:SINGLE POINT5:SELECTED EVENTS6:RETURN TO SETUP SCREEN
CH 1: ABSORBANCECH 2:CH 3:CH 4:DIG1:DIG2:MODE: TIME GRAPH-180
1:OK 3:ZERO2:CALIBRATE 4:SAVE/LOAD
CH 1: ABSORBANCE 0
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
Cuvette A
CH 1: ABSORBANCE 0
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings.
CAL
430nm
470nm 565nm
635nm
Previous Slide
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VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
Cuvette A
CH 1: ABSORBANCE 0
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
PRESS [ENTER] TO COLLECTOR [STO] TO STOP
1 0.052
CAL
430nm
470nm 565nm
635nm
Previous Slide
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Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to “ENTER VALUE?” Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
PRESS [ENTER] TO COLLECTOR [STO] TO STOP
1 0.052
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to “ENTER VALUE?” Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
PRESS [ENTER] TO COLLECTOR [STO] TO STOP
1 0.052
ENTER VALUE?PRESS [ENTER] TO COLLECTOR [STO] TO STOP
2 0.012
CAL
430nm
470nm 565nm
635nm
Previous Slide
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Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
PRESS [ENTER] TO COLLECTOR [STO] TO STOP
4 0.00025
Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu.
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
PRESS [ENTER] TO COLLECTOR [STO] TO STOP
4 0.00025
ABSORBANCE P1
X=concentration # y=Absorbance
CH 1: ABSORBANCE 0.009
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
CH 1: ABSORBANCE 0.009
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
CAL
430nm
470nm 565nm
635nm
Previous Slide
Replay
Continue
VERNIER
LabPro
Quick SetupStart/StopTransfer
Vernier
Power Supply
STAT PLOT F1 TBLSET F2 FORNAT F3 CALC F4 TABLE F5
Alpha
2ND Mode DEL
STAT
PRGM
COS
(
X,T,O,N
APPSMATH
7
4
1
0
8
5
2
.
9
6
3
(-)
x
+
-
÷
Enter
X -1
X2
LOG
LN
STO>
ON
SIN
,
VARS CLEAR
TAN
>
)
Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2.
TI-84 Silver EditionTexas Instruments
CAL
430nm
470nm 565nm
635nm
CH 1: ABSORBANCE 0.009
Mode: EVENTS WITH ENTRY
1: SETUP 4: ANALYZE2: START 5: TOOLS3: GRAPH 6: QUIT
EVENTS IN L1CH1 IN L2CH2 IN L3CH3 IN L4CH4 IN L5D IN L6, V In L7A IN L8 [ENTER]
End of Tutorial 3
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