RpoS mutants are confined to the midgut lumen and form round bodies

Bb distribution within Nymphal midguts isolated at 72 h post-placement

Hemolymph

WT-297 23/25

WT-B31 22/26

RpoS-297 0/40

BosR-297 0/17

BosR-B31 0/12

Epifluorescent Images

Round Body Formation in RPMI

Round Body Formation in RPMI

Bb strain 297

RpoS and RpoS-dependent upregulated gene transcripts decrease during RB formation

WT Strain 297

Round body recovery in nymphal midguts isolated at 72 h post-placement

BSK-IIRPMI