RNAi
Mitchell Planty
Quick Overview
RNAi– Natural regulatory mechanism for gene expression.
– Protection mechanism against overproduction of harmful
products
– Transmittable to other cells.
– Transmittable to offspring
dsRNA cell dsRNA + Dicer enzyme siRNA
siRNA + RISC mRNA = X
Useful?
Gene Identification
Gene Therapy
Delivery?
MSNs
M-MSNs
No negative effects
Example of Application
Aurora-A kinase
Laryngeal caner cell line Hep-2
Blocked by RNAi using a plasmid delivery
Suppressed Aurora-A production by 52%
Tumor size from Hep-2 line 7-10 cm
Tumor size from interfered line 1-3 cm
Works Cited
Campbell, Mary K., and Shawn O. Farrell. "13.8." Biochemistry. Belmont, CA: Brooks/Cole Cengage Learning, 2012. 385. Print.
"RCSB PDB - 1MQ4 Structure Summary." Web. 30 Mar. 2012. http://www.rcsb.org/pdb/explore/explore.do? structureId=1MQ4.
X, Li, Xie QR, Zhang J, and Gu H. "The Packaging of SiRNA within the Mesoporous Structure of Silica Nanoparticles." National Center for Biotechnology Information. U.S. National Library of
Medicine. Web. 30 Mar. 2012. http://www.ncbi.nlm.nih.gov/pubmed/21906804.
Zang, H., XH Chen, CP Cai, SL Wang, BY Liu, and L. Zhou. "[Suppression of Aurora-A by RNA Interference Inhibits Laryngeal Cancer Hep-2 Cell Growth]." National Center for Biotechnology Information. U.S. National Library of Medicine. Web. 30 Mar. 2012. <http://www.ncbi.nlm.nih.gov/pubmed/ 22455777>.
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