Bushehr University Of Medical Bushehr University Of Medical SciencesSciences
Dept. OF Pathology & Lab. Dept. OF Pathology & Lab. MedicineMedicine
Screening & Case Screening & Case FindingFinding
Diagnosis of DiseasesDiagnosis of Diseases
Therapeutic MonitoringTherapeutic Monitoring
Preanalytical stagePreanalytical stage
Analytical StageAnalytical Stage
Post analyticcal stagePost analyticcal stage
What is your clinical Impresion and D.D?What is your clinical Impresion and D.D?
What is the role of lab datWhat is the role of lab dataa in patient in patient management?management?
How is your Lab. Facilities?How is your Lab. Facilities?
What is the test ofWhat is the test of choice? choice?
The most effectiveThe most avaliableThe least costThe least invasion
cont .
Patient demographicsPatient demographics
Hospital numberHospital number
Brief historyBrief history date of referingdate of refering
List of requested TestList of requested Testss
Physition name & Signature Physition name & Signature
Diurnal variationsDiurnal variations -- smoking smoking
-- Ex Exccersize ersize - - Drugs Drugs
-- Fasting & Eating Fasting & Eating
-- Diet Diet - - Ethanol Ethanol
ExcersizeExcersize
CPKCPK
AST LDHAST LDH
Testostron Testostron
AndrostaAndrostanndiondionee
LHLH
FFAFFA
Alanin Alanin 180% 180%
Lactate 300%Lactate 300%
CPK , AST , LDHCPK , AST , LDH
Coagulation , Coagulation , PlatPlat
Transient effectsTransient effects Longterm effectsLongterm effects
High protein diet urea , UA , AmoniaHigh protein diet urea , UA , Amonia
U/S Fat. Diet CholestrolU/S Fat. Diet Cholestrol
Banana , Tomato , … Banana , Tomato , … SerotoninSerotonin
Beverage (Caffeine)Beverage (Caffeine)FFA , FFA , CatchalaminesCatchalamines
CortisolCortisol
CatecholamineCatecholaminess
FFAFFA
HbcoHbco HbHb Rbc countRbc count MCVMCV Leukocytosis Leukocytosis
(EOS)(EOS)
Biochemistry Hematology
- Liver function- Liver function
- Renal - Renal functionfunction
- …………- …………
- Preservatives- Preservatives
- Anticoagulants- Anticoagulants
- ……………- ……………
Physiologic effectsPhysiologic effects Invitro effectsInvitro effects
What is the specimen of What is the specimen of choice?choice?
What is the best method for What is the best method for SP. Collection?SP. Collection?
Posture & Position : Alb , Prot , Posture & Position : Alb , Prot , ca , Enzymes , Tca , Enzymes , TGG , Ch. , Ch.,,,,,,,,,,
Tourniquet : Ca , Lactate , Tourniquet : Ca , Lactate , Protein , EnzymesProtein , Enzymes
Stress & Anxiety : Hormones , Stress & Anxiety : Hormones , Wbc , Lactate , FFAWbc , Lactate , FFA
AnticoagulantsAnticoagulants
Protection from heat & coldProtection from heat & cold
Inhibition of contaminationInhibition of contamination
Transportation to Ref. LabTransportation to Ref. Lab
ObObttaining the best specimenaining the best specimen
Separation , centrifugationSeparation , centrifugation
Mixing , Rotation , ShakingMixing , Rotation , Shaking
WarWarmming & Boilinging & Boiling
MeasuringMeasuring
Cont …Cont …
NNature of specimenature of specimen Level of Lab. ManagingLevel of Lab. Managing Accessing Materials & Accessing Materials &
ReagentsReagents InstrumentationInstrumentation Pit falls & error resources and
economic benefits
InstrumentationInstrumentation
(Automated & Non (Automated & Non automated)automated)
Materials and MethodsMaterials and Methods
Techical personalsTechical personals
QQuality managementuality management Reporting Reporting
- Rapid (tat)- Rapid (tat)
- Accurate & precise- Accurate & precise
- Prompt- Prompt Lab. Information system Lab. Information system
(LIS)(LIS)
Pre analytical stagePre analytical stageAnalytical stageAnalytical stagePost analytical stagePost analytical stage
QA monitors quality performance from ordering to interpretation and application of results in patient care.
Assay of Control SampleAssay of Control SampleInstrument MaintenanceInstrument MaintenanceStatistical data analysisStatistical data analysisProficiency testing surveyProficiency testing survey
Pre analytical Pre analytical
Analytical Analytical
Post analytical Post analytical
80%80%
15%15%
5%5%
Clinical ImpressionClinical Impression Sensitivity & specificity of Sensitivity & specificity of
TestTest Limitation of TestLimitation of Test Reproducibility of TestReproducibility of Test Lab. Quality assuranceLab. Quality assurance
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